About: Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests

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About: Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests
Creator	Joachim, Anja Hinney, Barbara Lichtmannsperger, Katharina Wittek, Thomas
Source	Elsevier; Medline; PMC
abstract	Abstract To obtain information about the occurrence and genotype distribution of G. intestinalis and C. parvum in Austrian cattle, faecal samples from diarrhoeic calves younger than 180 days of age originating from 70 farms were examined. Of the 177 faecal samples, 27.1% were positive for Giardia cysts (immunofluorescence microscopy) and 55.4% for Cryptosporidium oocysts (phase-contrast microscopy). Positive samples were characterized by nested PCR for Giardia, 83.3% (triosephosphate isomerase; tpi) and 89.6% (β-giardin; bg) were positive, while the Cryptosporidium nested PCR returned 92.5% (60-kDa glycoprotein) positive results. Sequence analysis revealed one assemblage A-positive sample and 30 (bg) respectively 29 (tpi) assemblage E-positive samples for G. intestinalis. For C. parvum four subtypes within the IIa family (IIaA15G2R1, n = 29; IIaA19G2R2, n = 3; IIaA21G2R1, n = 2; IIaA14G1R1, n = 1) could be differentiated. Validation of two immunochromatographic point-of-care tests resulted in a sensitivity of 29.2% and 77.6%; a specificity of 98.4% and 91.1% for the detection of Giardia intestinalis and Cryptosporidium parvum, respectively. Results confirm the widespread occurrence of both protozoa in diarrhoeic calves in Austria.
has issue date	2019-10-31(xsd:dateTime)
bibo:doi	10.1016/j.cimid.2019.101333
bibo:pmid	31437689
has license	els-covid
sha1sum (hex)	439c3062b9159bb0a65247ff417f4a834f64c7e0
schema:url	https://doi.org/10.1016/j.cimid.2019.101333
resource representing a document's title	Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests
has PubMed Central identifier	PMC7112675
has PubMed identifier	31437689
schema:publication	Comparative Immunology, Microbiology and Infectious Diseases
resource representing a document's body	covid:439c3062b9159bb0a65247ff417f4a834f64c7e0#body_text
is schema:about of	named entity 'AUSTRIA' named entity 'Giardia intestinalis' named entity 'family' named entity 'returned' named entity 'Giardia' named entity 'detection' named entity 'CRYPTOSPORIDIUM PARVUM' named entity 'TESTS' named entity 'AUSTRIA' named entity 'CRYPTOSPORIDIUM PARVUM' named entity 'REVEALED' named entity 'INFORMATION' named entity 'VALIDATION' named entity 'MICROSCOPY' named entity 'COULD BE' named entity 'DISTRIBUTION' named entity 'CATTLE' named entity 'GIARDIA INTESTINALIS' named entity 'SPECIFICITY' named entity 'RESULTS' named entity 'POSITIVE' named entity 'OCCURRENCE' named entity 'EXAMINED' named entity 'KDA' named entity 'GENOTYPE' named entity 'ISOMERASE' named entity 'CRYPTOSPORIDIUM' named entity 'IIA' named entity 'SAMPLES' named entity 'GIARDIA' named entity 'PROTOZOA' named entity 'CONTRAST' named entity 'CALVES' named entity 'CHARACTERIZED' named entity 'YOUNGER' named entity 'DIFFERENTIATED' named entity 'CYSTS' named entity 'SEQUENCE ANALYSIS' named entity 'POINT-OF-CARE' named entity 'WIDESPREAD' named entity 'TESTS' named entity 'DIARRHOEA' named entity 'POINT-OF-CARE' named entity 'CALVES' named entity 'GIARDIA INTESTINALIS' named entity 'AGE' named entity 'CONFIRM' named entity 'ABOUT' named entity '2860' named entity 'TPI' named entity 'NESTED PCR ' named entity 'POSITIVE FOR' named entity 'AUSTRIAN' named entity 'SUBTYPES' named entity 'RETURNED' named entity 'SAMPLE' named entity 'C. PARVUM' named entity 'OOCYSTS' named entity 'GLYCOPROTEIN' named entity 'FAMILY' named entity 'DETECTION' named entity 'CHARACTERIZATION' named entity 'OBTAIN' named entity 'FARMS' named entity 'DAYS' named entity 'EVALUATION' named entity 'MOLECULAR' named entity 'SENSITIVITY' named entity 'FAECAL' named entity '180' covid:arg/439c3062b9159bb0a65247ff417f4a834f64c7e0 named entity 'cysts' named entity 'Cryptosporidium parvum' named entity 'nested PCR' named entity 'Austria' named entity 'genotype' named entity 'Molecular' named entity 'Cryptosporidium'

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