About: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV

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About: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV
Creator	Bols, Niels Marshall, Sergio Peña, Andrea
Source	Medline; PMC
abstract	BACKGROUND: Due to the limited number of species specific antibodies against fish proteins, differential gene expression analyses are vital for the study of host immune responses. Quantitative real-time reverse transcription PCR (qRT-PCR) is one of the most powerful tools for this purpose. Nevertheless, the accuracy of the method will depend on the careful selection of genes whose expression are stable and can be used as internal controls for a particular experimental setting. FINDINGS: The expression stability of five commonly used housekeeping genes [beta-actin (ACTB), elongation factor 1-alpha (EF1A), ubiquitin (UBQ), glyceraldehyd-3-phosphate dehydrogenase (GAPDH) and tubulin alpha (TUBA)] were monitored in salmonid cell lines CHSE-214 and RTS11 after infection with two of the most fastidious fish pathogens, the facultative bacterium Piscirickettsia salmonis and the aquabirnavirus IPNV (Infectious Pancreatic Necrosis Virus). After geNorm analysis, UBQ and EF1A appeared as the most stable, although EF1A was slightly upregulated at late stages of P. salmonis infection in RTS11. ACTB instead, showed a good performance in each case, being always considered within the three most stable genes of the panel. In contrast, infection-dependent differential regulation of GAPDH and TUBA was also demonstrated. CONCLUSION: Based on the data presented here with the cell culture models CHSE-214 and RTS11, we suggest the initial choice of UBQ, ACTB and EF1A as reference genes in qRT-PCR assays for studying the effect of P. salmonis and IPNV on the host immune response.
has issue date	2010-04-14(xsd:dateTime)
bibo:doi	10.1186/1756-0500-3-101
bibo:pmid	20398263
has license	cc-by
sha1sum (hex)	43eaa4d7209e389a360aeab2b3b44e76fd89f5c2
schema:url	https://doi.org/10.1186/1756-0500-3-101
resource representing a document's title	An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV
has PubMed Central identifier	PMC2873344
has PubMed identifier	20398263
schema:publication	BMC Res Notes
resource representing a document's body	covid:43eaa4d7209e389a360aeab2b3b44e76fd89f5c2#body_text
is schema:about of	named entity 'PISCIRICKETTSIA SALMONIS' named entity 'IPNV' named entity 'facultative' named entity 'Infectious Pancreatic Necrosis Virus' named entity 'bacterium' named entity 'stable' named entity 'real-time' named entity 'REFERENCE' covid:arg/43eaa4d7209e389a360aeab2b3b44e76fd89f5c2 named entity 'STUDY' named entity 'DEPEND' named entity 'REAL-TIME' named entity 'LIMITED' named entity 'DEPENDENT' named entity 'CELL LINES' named entity 'CONTRAST' named entity 'EF1A' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'IMMUNE RESPONSES' named entity 'HOUSEKEEPING GENES' named entity 'VIRUS' named entity 'UPREGULATED' named entity 'ANALYSIS' named entity 'CHSE-214' named entity 'PCR' named entity 'ONE OF' named entity 'STABILITY' named entity 'ELONGATION FACTOR 1-ALPHA' named entity 'NUMBER OF' named entity 'PISCIRICKETTSIA SALMONIS' named entity 'DIFFERENTIAL' named entity 'PATHOGENS' named entity 'STABILITY' named entity 'IPNV' named entity 'PARTICULAR' named entity 'BACKGROUND' named entity 'UBQ' named entity 'USED' named entity 'TOOLS' named entity 'UBIQUITIN' named entity 'PURPOSE' named entity 'DUE TO' named entity 'DEHYDROGENASE' named entity 'PANCREATIC NECROSIS' named entity 'MONITORED' named entity 'POWERFUL' named entity 'GENES' named entity 'TUBULIN ALPHA' named entity 'GLYCERALDEHYD' named entity 'EXPERIMENTAL SETTING' named entity 'CELL LINES' named entity 'TUBA' named entity 'PHOSPHATE' named entity 'LATE STAGES' named entity 'ACCURACY' named entity 'CONSIDERED' named entity 'FISH PROTEINS' named entity 'SPECIES' named entity 'DIFFERENTIAL GENE EXPRESSION' named entity 'AQUABIRNAVIRUS' named entity 'PERFORMANCE' named entity 'ACTIN' named entity 'REGULATION' named entity 'SLIGHTLY' named entity 'HOST' named entity 'SALMONID' named entity 'GENES' named entity 'BEING' named entity 'EXPRESSION' named entity 'POTENTIAL' named entity 'EVALUATION' named entity 'PANEL' named entity 'QUANTITATIVE' named entity 'SPECIFIC' named entity 'SELECTION' named entity 'FINDINGS' named entity 'METHOD' named entity 'INFECTION' named entity 'SALMONID'

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