About: Avian antibodies (IgY) against Trypanosoma cruzi: Purification and characterization studies

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Avian antibodies (IgY) against Trypanosoma cruzi: Purification and characterization studies
Creator	Aguirre, Gisele Azevedo, Maria Baldissera, Matheus De Jesus, Francielli De Sá, Mariângela Do Carmo, Guilherme Gonzalez Monteiro, Silvia Grando, Thirssa Moura, Lenita Porto, Carolina Sagrillo, Michele Santurio, Janio
source	Elsevier; Medline; PMC
abstract	Abstract Trypanosoma cruzi is a flagellated protozoan belonging to the Trypanosomatidae family, the etiologic agent of Chagas disease. Currently, there is neither a licensed vaccine nor effective treatment, characterizing an unmet clinical need. The IgY refers to the egg yolk immunoglobulin (Y=yolk) and its production and use are subjects of many studies due to the diversity of its diagnostic and therapeutic applications. Several researchers have shown that the use of specific IgY may prevent and/or control infectious and parasitic diseases. Based on these evidences, the aim of this study was to immunize chickens with trypomastigotes of T. cruzi in order to produce highly effective and pure antibodies (IgY), as well as extract, characterize, quantify, and verify cytotoxic effects of IgY anti-T. cruzi. After the induction of IgY production by chickens, the eggs were collected and the IgY was extracted by method of precipitation of polyethylene glycol 6000. The IgY anti-T. cruzi characterization was performed using polyacrylamide gel electrophoresis (SDS-PAGE), western-blot and enzyme-linked immunosorbent assay (ELISA). Moreover, the cytotoxic or proliferative effects of IgY anti-T. cruzi was verified by MTT assay. The concentration of IgY in yolk was 8.41±1.47mg/mL. The characterization of IgY reveled bands of stained peptides with molecular weight between 75 and 50kDa and 37 and 25kDa. In the ELISA test was observed that there was antigen-antibody reaction throughout the sample period. The concentrations of 1, 5 and 10mg/mL of IgY anti-T. cruzi presented no cytotoxic of proliferative effects in mononuclear and VERO cells in vitro. The results indicated that T. cruzi is able to generate a high production of specific immunoglobulins in chickens, it did not cause damage to the cell membrane and no proliferative effect.
has issue date	2017-10-31(xsd:dateTime)
bibo:doi	10.1016/j.jim.2017.07.002
bibo:pmid	28697990
has license	els-covid
sha1sum (hex)	4469e939c333b6b38d126b2d54be344ad299cf28
schema:url	https://doi.org/10.1016/j.jim.2017.07.002
resource representing a document's title	Avian antibodies (IgY) against Trypanosoma cruzi: Purification and characterization studies
has PubMed Central identifier	PMC7126890
has PubMed identifier	28697990
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:4469e939c333b6b38d126b2d54be344ad299cf28#body_text
is schema:about of	named entity 'STUDIES' named entity 'Trypanosomatidae' named entity 'protozoan' named entity 'cytotoxic' named entity 'proliferative' named entity 'Trypanosoma' named entity 'studies' named entity 'MONONUCLEAR' named entity 'CONCENTRATIONS' named entity 'DIVERSITY' named entity 'EGG YOLK' named entity 'HIGH' named entity 'METHOD' named entity 'SAMPLE' named entity 'PAGE' named entity 'CURRENTLY' named entity 'UNMET' named entity 'RESEARCHERS' named entity 'EFFECT' named entity 'BELONGING' named entity 'ANTIBODIES' named entity 'PERIOD' named entity 'TEST' named entity 'THERAPEUTIC' named entity 'CHARACTERIZATION' named entity 'STAINED' named entity 'BLOT' named entity 'PRECIPITATION' named entity 'CELL MEMBRANE' named entity 'CONCENTRATION OF' named entity 'TRYPANOSOMA CRUZI' named entity 'ANTIBODIES' named entity 'AVIAN' named entity 'FLAGELLATED' named entity 'INFECTIOUS AND PARASITIC DISEASES' named entity 'TRYPOMASTIGOTES' named entity 'STUDY' named entity 'POLYACRYLAMIDE GEL ELECTROPHORESIS' named entity 'ITS' named entity 'DUE TO' named entity 'ETIOLOGIC AGENT' named entity 'GENERATE' named entity 'HIGHLY' named entity 'INDICATED' named entity 'ORDER' named entity 'TRYPANOSOMA CRUZI' named entity 'POLYETHYLENE GLYCOL 6000' named entity 'EGGS' named entity 'INDUCTION' named entity 'LICENSED VACCINE' named entity 'THESE' named entity 'CYTOTOXIC' named entity 'STUDIES' named entity 'USE OF' named entity 'DIAGNOSTIC' named entity 'TO PRODUCE' named entity 'FAMILY' named entity 'EFFECTS' named entity 'PERFORMED' named entity 'PURIFICATION' named entity 'YOLK' named entity 'USING' named entity 'CONTROL' named entity 'IN VITRO' named entity 'PROTOZOAN' named entity 'NEED' named entity 'CLINICAL' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'HAVE' named entity 'EXTRACTED'

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