About: Electron Tomography Reveals the Steps in Filovirus Budding

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Electron Tomography Reveals the Steps in Filovirus Budding
Creator	Becker, S Kolesnikova, L Krähling, V Riches, J Welsch, S
Source	PMC
abstract	The filoviruses, Marburg and Ebola, are non-segmented negative-strand RNA viruses causing severe hemorrhagic fever with high mortality rates in humans and nonhuman primates. The sequence of events that leads to release of filovirus particles from cells is poorly understood. Two contrasting mechanisms have been proposed, one proceeding via a “submarine-like” budding with the helical nucleocapsid emerging parallel to the plasma membrane, and the other via perpendicular “rocket-like” protrusion. Here we have infected cells with Marburg virus under BSL-4 containment conditions, and reconstructed the sequence of steps in the budding process in three dimensions using electron tomography of plastic-embedded cells. We find that highly infectious filamentous particles are released at early stages in infection. Budding proceeds via lateral association of intracellular nucleocapsid along its whole length with the plasma membrane, followed by rapid envelopment initiated at one end of the nucleocapsid, leading to a protruding intermediate. Scission results in local membrane instability at the rear of the virus. After prolonged infection, increased vesiculation of the plasma membrane correlates with changes in shape and infectivity of released viruses. Our observations demonstrate a cellular determinant of virus shape. They reconcile the contrasting models of filovirus budding and allow us to describe the sequence of events taking place during budding and release of Marburg virus. We propose that this represents a general sequence of events also followed by other filamentous and rod-shaped viruses.
has issue date	2010-04-29(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1000875
bibo:pmid	20442788
has license	cc-by
sha1sum (hex)	44ab6aecdacb870226ce4ce36606e227f0a6ac00
schema:url	https://doi.org/10.1371/journal.ppat.1000875
resource representing a document's title	Electron Tomography Reveals the Steps in Filovirus Budding
has PubMed Central identifier	PMC2861712
has PubMed identifier	20442788
schema:publication	PLoS Pathog
resource representing a document's body	covid:44ab6aecdacb870226ce4ce36606e227f0a6ac00#body_text
is schema:about of	named entity 'contrasting' named entity 'contrasting' named entity 'causing' named entity 'release' named entity 'shape' named entity 'Budding' covid:arg/44ab6aecdacb870226ce4ce36606e227f0a6ac00 named entity 'ELECTRON TOMOGRAPHY' named entity 'RELEASE' named entity 'TO DESCRIBE' named entity 'MEMBRANE' named entity 'CAUSING' named entity 'RAPID' named entity 'MARBURG VIRUS' named entity 'VESICULATION ' named entity 'VIRUSES' named entity 'INFECTIVITY' named entity 'LIKE' named entity 'MODELS' named entity 'SEGMENTED ' named entity 'NUCLEOCAPSID' named entity 'CONTAINMENT' named entity 'PARALLEL TO' named entity 'SHAPE' named entity 'FILOVIRUSES' named entity 'RELEASED' named entity 'VIRUS' named entity 'MECHANISMS' named entity 'ITS' named entity 'PROLONGED' named entity 'HIGH' named entity 'OUR' named entity 'HEMORRHAGIC FEVER' named entity 'OBSERVATIONS' named entity 'NEGATIVE' named entity 'EVENTS' named entity 'INFECTION' named entity 'INSTABILITY' named entity 'INTERMEDIATE' named entity 'LEADS' named entity 'STRAND' named entity 'INFECTIOUS' named entity 'LENGTH' named entity 'RECONCILE' named entity 'LEADING' named entity 'ELECTRON TOMOGRAPHY' named entity 'CONDITIONS' named entity 'FILOVIRUS' named entity 'CHANGES' named entity 'CELLS' named entity 'ASSOCIATION' named entity 'MORTALITY RATES' named entity 'STEPS' named entity 'END' named entity 'EMBEDDED' named entity 'PROTRUSION' named entity 'TAKING' named entity 'PERPENDICULAR' named entity 'CONTRASTING' named entity 'CELLULAR' named entity 'FOLLOWED BY' named entity 'SEVERE' named entity 'PARTICLES' named entity 'DETERMINANT' named entity 'LOCAL' named entity 'ALLOW' named entity 'EBOLA' named entity 'HELICAL' named entity 'BUDDING' named entity 'FILOVIRUS' named entity 'UNDERSTOOD' named entity 'NONHUMAN PRIMATES' named entity 'FILAMENTOUS' named entity 'PROTRUDING ' named entity 'PLACE' named entity 'INTRACELLULAR' named entity 'USING'

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