About: Neuropilin-1 is a host factor for SARS-CoV-2 infection

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About: Neuropilin-1 is a host factor for SARS-CoV-2 infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Neuropilin-1 is a host factor for SARS-CoV-2 infection
Creator	Helenius, Ari Bauer, Michael Davidson, Andrew Hiscox, Julian Matthews, David Greber, Urs Shoemark, Deborah Sessions, Richard Yamauchi, Yohei Teesalu, Tambet Horvath, Peter Antón-Plágaro, Carlos Cullen, Peter Daly, James Hollandi, Reka Kavanagh Williamson, Maia Klein, Katja Simonetti, Boris Simón-Gracia, Lorena
Source	BioRxiv
abstract	SARS-CoV-2 is the causative agent of COVID-19, a coronavirus disease that has infected more than 6.6 million people and caused over 390,000 deaths worldwide1,2. The Spike (S) protein of the virus forms projections on the virion surface responsible for host cell attachment and penetration. This viral glycoprotein is synthesized as a precursor in infected cells and, to be active, must be cleaved to two associated polypeptides: S1 and S2(3,4). For SARS-CoV-2 the cleavage is catalysed by furin, a host cell protease, which cleaves the S protein precursor at a specific sequence motif that generates a polybasic Arg-Arg-Ala-Arg (RRAR) C-terminal sequence on S1. This sequence motif conforms to the C-end rule (CendR), which means that the C-terminal sequence may allow the protein to associate with cell surface neuropilin-1 (NRP1) and neuropilin-2 (NRP2) receptors5. Here we demonstrate using immunoprecipitation, site-specific mutagenesis, structural modelling, and antibody blockade that, in addition to engaging the known receptor ACE2, S1 can bind to NRP1 through the canonical CendR mechanism. This interaction enhances infection by SARS-CoV-2 in cell culture. NRP1 thus serves as a host factor for SARS-CoV-2 infection, and provides a therapeutic target for COVID-19.
has issue date	2020-06-05(xsd:dateTime)
bibo:doi	10.1101/2020.06.05.134114
has license	biorxiv
sha1sum (hex)	4530a08febf1b5fcd9aefc9275dba9d8009fc438
schema:url	https://doi.org/10.1101/2020.06.05.134114
resource representing a document's title	Neuropilin-1 is a host factor for SARS-CoV-2 infection
schema:publication	bioRxiv
resource representing a document's body	covid:4530a08febf1b5fcd9aefc9275dba9d8009fc438#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'supervised' named entity 'Caco-2' named entity 'ACE2' named entity 'mCherry' named entity 'Odyssey software' named entity 'NRP1' named entity 'Tecan' named entity 'NRP2' named entity 'immunoprecipitation' named entity 'NRP1' named entity 'NRP1' named entity 'mCherry' named entity 'Wild type' named entity 'ACE2' named entity 'mCherry' named entity 'HeLa' named entity 'NRP1' named entity 'HeLa' named entity 'NRP1' named entity 'NRP1' named entity 'Cell entry' named entity 'GFP' named entity 'protein binding' named entity 'HEK293T' named entity 'HeLa' named entity 'vector' named entity 'transport medium' named entity 'SARS-CoV2' named entity 'infection' named entity 'mCherry' named entity 'transiently transfected' named entity 'GFP' named entity 'phenotype' named entity 'GFP' named entity 'GFP' named entity 'NRP1' named entity 'puromycin' named entity 'GFP' named entity 'University of Liverpool' named entity 'Triton X-100' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'plasmids' named entity 'cell lines' named entity 'SARS-CoV-2' named entity 'Coimmunoprecipitation' named entity 'NRP1' named entity 'complementary binding' named entity 'Nrp1' named entity 'CO2' named entity 'interact' named entity 'Nrp1' named entity 'SARS-CoV-2' named entity 'HEK293T cells' named entity 'lentiviral particles' named entity 'Wuhan' named entity 'ligand' named entity 'microplate reader' named entity 'protein' named entity 'VEGF' named entity 'A164' named entity 'Site-directed mutagenesis' named entity 'NRP2' named entity 'syncytia' named entity 'antibody'

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