About: Poly‐L‐lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Poly‐L‐lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection
Creator	Bayramlı, Yaşar Kar, Dü Kuralay, Filiz Nilgü, ]
Source	PMC
abstract	Poly‐L‐lysine is one of the biocompatible polymers having amino and carboxyl groups in its structure. This attractive feature of poly‐L‐lysine makes it very convenient for bioactive molecule attachment. This study details the preparation of poly‐L‐lysine‐based pencil graphite electrodes (PLL/PGEs) and use of the coated electrodes for direct ultrasensitive DNA hybridization detection. In the first part of this study, poly‐L‐lysine coated electrodes were prepared using L‐lysine as the monomer by cyclic voltammetry (CV) with different cyclic scans. The effect of these cyclic scans during the electropolymerization was investigated. Coated electrodes were characterized by cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Then, one‐pot preparation of poly‐L‐lysine composites with graphene (GN) and multi‐walled carbon nanotubes (MWCNTs) onto the pencil graphite electrodes were achieved. Electrochemical responses of these 3 electrodes were compared. After all, electrochemical DNA hybridization was performed using the poly‐L‐lysine‐based electrodes prepared at optimum polymerization condition. The PLL/PGE coated electrode presented a good linear response in the target concentration range of 1.0×10(−13) to 1.0×10(−6) with a detection limit of 2.25×10(−14) using differential pulse voltammetry as the detection method. We believe that poly‐L‐lysine‐based surfaces will be useful for further clinical applications.
has issue date	2018-05-24(xsd:dateTime)
bibo:doi	10.1002/elan.201800153
has license	no-cc
sha1sum (hex)	45f44734e2758806e5ef69381f03bc5687a8a1bb
schema:url	https://doi.org/10.1002/elan.201800153
resource representing a document's title	Poly‐L‐lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection
has PubMed Central identifier	PMC7163579
schema:publication	Electroanalysis
resource representing a document's body	covid:45f44734e2758806e5ef69381f03bc5687a8a1bb#body_text
is schema:about of	named entity 'INVESTIGATED' named entity 'DNA hybridization' named entity 'one-pot' named entity 'molecule' named entity 'graphene' named entity 'EIS' named entity 'PLL' named entity 'poly-L-lysine' named entity 'graphite electrodes' named entity 'cyclic voltammetry' named entity 'poly-L-lysine' named entity 'Poly-L-lysine' named entity 'electrochemical' named entity 'DNA hybridization' named entity 'electrodes' named entity 'PGE' named entity 'guanine' named entity 'guanine' named entity 'electrochemical impedance spectroscopy' named entity 'PLL' named entity 'graphene' named entity 'electrochemical' named entity 'SEM' named entity 'electrodes' named entity 'PLL' named entity 'PCR' named entity 'hybridization' named entity 'AgCl' named entity 'sodium chloride' named entity 'electrochemical' named entity 'anion' named entity 'cyclic voltammetry' named entity 'severe acute respiratory syndrome' named entity 'nucleic acid' named entity 'methylene blue' named entity 'molecular biology' named entity 'pathogen' named entity 'biosensor' named entity 'single walled carbon nanotubes' named entity 'polymerization' named entity 'counter electrode' named entity 'lower current' named entity 'electrode' named entity 'PGEs' named entity 'reagent grade' named entity 'gene' named entity 'Turkey' named entity 'guanine' named entity 'PLL' named entity 'electrode' named entity 'PLL' named entity 'cathodic' named entity 'electrodes' named entity 'genetic information' named entity 'biosensor' named entity 'electropolymerized' named entity 'PLL' named entity 'polymerization' named entity 'DNA hybridization' named entity 'oxidation' named entity 'dsDNA' named entity 'Tombow' named entity 'nitrogen gas' named entity 'gene' named entity 'PPy' named entity 'nanomaterials' named entity 'electrode' named entity 'nitrogen gas' named entity 'acetate' named entity 'pH 4' named entity 'human plasma' named entity 'Biosensors' named entity 'detection limit' named entity 'serum samples' named entity 'carbon electrodes' named entity 'poly-L-lysine' named entity 'cytosine' named entity 'SEM' named entity 'biotechnology' named entity 'nanocomposites'

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