About: Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription

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About: Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription
Creator	Palù, Giorgio Butovskaya, Elena Frasson, Ilaria Nadai, Matteo Perrone, Rosalba Scalabrin, Matteo Fabris, Dan Tosoni, Elena Richter, Sara
Source	Medline; PMC
abstract	Folding of the LTR promoter into dynamic G-quadruplex conformations has been shown to suppress its transcriptional activity in HIV-1. Here we sought to identify the proteins that control the folding of this region of proviral genome by inducing/stabilizing G-quadruplex structures. The implementation of electrophorethic mobility shift assay and pull-down experiments coupled with mass spectrometric analysis revealed that the cellular protein nucleolin is able to specifically recognize G-quadruplex structures present in the LTR promoter. Nucleolin recognized with high affinity and specificity the majority, but not all the possible G-quadruplexes folded by this sequence. In addition, it displayed greater binding preference towards DNA than RNA G-quadruplexes, thus indicating two levels of selectivity based on the sequence and nature of the target. The interaction translated into stabilization of the LTR G-quadruplexes and increased promoter silencing activity; in contrast, disruption of nucleolin binding in cells by both siRNAs and a nucleolin binding aptamer greatly increased LTR promoter activity. These data indicate that nucleolin possesses a specific and regulated activity toward the HIV-1 LTR promoter, which is mediated by G-quadruplexes. These observations provide new essential insights into viral transcription and a possible low mutagenic target for antiretroviral therapy.
has issue date	2015-10-15(xsd:dateTime)
bibo:doi	10.1093/nar/gkv897
bibo:pmid	26354862
has license	cc-by
sha1sum (hex)	468cd07dc546d39f95529846f303e76f02ae51b3
schema:url	https://doi.org/10.1093/nar/gkv897
resource representing a document's title	Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription
has PubMed Central identifier	PMC4605322
has PubMed identifier	26354862
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:468cd07dc546d39f95529846f303e76f02ae51b3#body_text
is schema:about of	named entity 'promoter' named entity 'regulated' named entity 'essential' named entity 'nature' named entity 'identify' named entity 'binding' named entity 'HIV-1' covid:arg/468cd07dc546d39f95529846f303e76f02ae51b3 named entity 'INTERACTION' named entity 'G-QUADRUPLEX' named entity 'SELECTIVITY' named entity 'PROVIDE' named entity 'MAJORITY' named entity 'SEQUENCE' named entity 'SPECIFIC' named entity 'GENOME' named entity 'ESSENTIAL' named entity 'FOLDING' named entity 'BASED' named entity 'RECOGNIZE' named entity 'TRANSLATED' named entity 'OBSERVATIONS' named entity 'PULL' named entity 'LOW' named entity 'CONTRAST' named entity 'GREATLY' named entity 'SUPPRESS' named entity 'BUT' named entity 'PROTEINS' named entity 'CELLS' named entity 'AFFINITY' named entity 'ANTIRETROVIRAL THERAPY' named entity 'TRANSCRIPTIONAL ACTIVITY' named entity 'STABILIZATION' named entity 'INDUCING' named entity 'REGULATED' named entity 'REGION' named entity 'POSSIBLE' named entity 'LEVELS' named entity 'PROMOTER ACTIVITY' named entity 'NATURE' named entity 'ANALYSIS' named entity 'NEW' named entity 'APTAMER' named entity 'TO IDENTIFY' named entity 'G-QUADRUPLEX' named entity 'MUTAGENIC' named entity 'VIRAL TRANSCRIPTION' named entity 'ACTIVITY' named entity 'TARGET' named entity 'INSIGHTS' named entity 'THESE' named entity 'BINDING' named entity 'CONTROL' named entity 'EXPERIMENTS' named entity 'RNA G-QUADRUPLEXES' named entity 'HIV-1' named entity 'SIRNAS' named entity 'SILENCING' named entity 'DYNAMIC' named entity 'FOLDED' named entity 'NUCLEOLIN' named entity 'PROMOTER' named entity 'STABILIZES' named entity 'STRUCTURES' named entity 'PROMOTER' named entity 'LTR' named entity 'HERE' named entity 'DNA' named entity 'LTR' named entity 'MOBILITY SHIFT ASSAY' named entity 'HIGH' named entity 'IMPLEMENTATION'

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