About: Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1

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About: Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1
Creator	Lu, Yan Zhang, Wei Wang, Yang Lu, Chengping Yao, Huochun Tang, Fang Shao, Jing Liu, Guangjin Wu, Zongfu Zhai, Zhipeng
Source	Medline; PMC
abstract	BACKGROUND: Actinobacillus pleuropneumoniae (APP) is one of the most important swine pathogens worldwide. Identification and characterization of novel antigenic APP vaccine candidates are underway. In the present study, we use an immunoproteomic approach to identify APP protein antigens that may elicit an immune response in serotype 1 naturally infected swine and serotype 1 virulent strain S259-immunized rabbits. RESULTS: Proteins from total cell lysates of serotype 1 APP were separated by two-dimensional electrophoresis (2DE). Western blot analysis revealed 21 immunoreactive protein spots separated in the pH 4-7 range and 4 spots in the pH 7-11 range with the convalescent sera from swine; we found 5 immunoreactive protein spots that separated in the pH 4-7 range and 2 in the pH 7-11 range with hyperimmune sera from S259-immunized rabbits. The proteins included the known antigens ApxIIA, protective surface antigen D15, outer membrane proteins P5, subunit NqrA. The remaining antigens are being reported as immunoreactive proteins in APP for the first time, to our knowledge. CONCLUSIONS: We identified a total of 42 immunoreactive proteins of the APP serotype 1 virulent strain S259 which represented 32 different proteins, including some novel immunoreactive factors which could be researched as vaccine candidates.
has issue date	2011-06-26(xsd:dateTime)
bibo:doi	10.1186/1477-5956-9-32
bibo:pmid	21703014
has license	cc-by
sha1sum (hex)	47fb645312a069e65bb7557c58204244c3c92953
schema:url	https://doi.org/10.1186/1477-5956-9-32
resource representing a document's title	Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1
has PubMed Central identifier	PMC3148531
has PubMed identifier	21703014
schema:publication	Proteome Sci
resource representing a document's body	covid:47fb645312a069e65bb7557c58204244c3c92953#body_text
is schema:about of	named entity 'SEROTYPE' named entity 'IMMUNE RESPONSE' named entity 'PRESENT' named entity 'USE' named entity 'APPROACH' named entity 'VIRULENT STRAIN' named entity 'INFECTED' named entity 'NOVEL' named entity 'STUDY' named entity 'SWINE' named entity 'PROTEIN ' named entity 'naturally' named entity 'swine' covid:arg/47fb645312a069e65bb7557c58204244c3c92953 named entity 'ELICIT' named entity 'WORLDWIDE' named entity 'IMPORTANT' named entity 'APP' named entity 'BACKGROUND' named entity 'ANALYSIS' named entity 'BACTERIAL PROTEINS' named entity 'ACTINOBACILLUS PLEUROPNEUMONIAE' named entity 'IDENTIFICATION' named entity 'PATHOGENS' named entity 'VACCINE' named entity 'ACTINOBACILLUS PLEUROPNEUMONIAE SEROTYPE 1' named entity 'IMMUNIZED' named entity 'TO IDENTIFY' named entity 'ANTIGENS' named entity 'RABBITS' named entity 'ONE OF' named entity 'CHARACTERIZATION' named entity 'vaccine' named entity 'virulent' named entity 'serotype' named entity 'antigenic' named entity 'elicit an immune response' named entity 'pathogens' named entity 'western blot' named entity 'homologous sequences' named entity 'bromophenol blue' named entity 'highly conserved' named entity 'serotype' named entity 'IPG' named entity 'pathogens' named entity 'IPG' named entity 'intermediate state' named entity 'China' named entity 'Shanghai' named entity 'western blot' named entity 'Nanjing' named entity 'China' named entity 'serotypes' named entity 'SDS-PAGE' named entity 'isoforms' named entity 'China' named entity 'immunoreactive' named entity 'swine' named entity 'Escherichia coli' named entity 'live-attenuated' named entity 'serovar' named entity 'MALDI-TOF MS' named entity 'Trypticase Soy Broth' named entity 'Actinobacillus pleuropneumoniae' named entity 'insoluble' named entity 'intermediate state' named entity 'convalescent serum' named entity 'swine' named entity 'immunoreactive' named entity 'immunoblot' named entity 'IPG' named entity 'proteome'

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