About: Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification
Creator	Yuan, Wanzhe Wang, Jinfeng Liu, Libing Wang, Jianchang Zhang, Ruoxi Shi, Ruihan Han, Qingan Yuan, W
Source	Elsevier; Medline; PMC
abstract	Abstract A rapid and specific real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA) was developed to detect the transmissible gastroenteritis virus (TGEV) in this study. The primers and exo probe were designed to be specific for a portion of spike (S) gene conserved in TGEV, but absent in the closely related porcine respiratory coronavirus (PRCV). The amplification was performed at 40 °C for 20 min. The assay could only detect the TGEV, and there was no cross-reaction with other pathogens tested. Using the in vitro transcribed TGEV RNA as template, the limit of detection of the developed RT-RPA was 100 copies per reaction. The assay performance was evaluated by testing 76 clinical samples by RT-RPA and a real-time RT-PCR. Fourteen samples were TGEV RNA positive in RT-RPA (18.4%, 14/76), which were also positive in the real-time RT-PCR. The diagnostic agreement between the two assays was 100% (76/76). The R2 value of RT-RPA and real-time RT-PCR was 0.959 by linear regression analysis. The developed RT-RPA assay provides a useful alternative tool for rapid, simple and reliable detection of TGEV in resource-limited diagnostic laboratories and on-site facilities.
has issue date	2018-06-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.03.005
bibo:pmid	29550352
has license	els-covid
sha1sum (hex)	480eba2a349789e138faa586879ebd479b068193
schema:url	https://doi.org/10.1016/j.jviromet.2018.03.005
resource representing a document's title	Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification
has PubMed Central identifier	PMC7113865
has PubMed identifier	29550352
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:480eba2a349789e138faa586879ebd479b068193#body_text
is schema:about of	named entity 'SWINE' named entity 'assay' named entity 'samples' named entity 'simple' named entity 'exo' named entity 'porcine' named entity 'samples' named entity 'REAL-TIME' named entity 'TOOL' named entity 'TESTING' named entity 'CROSS-REACTION' named entity '2818' named entity 'SPIKE' named entity 'IN VITRO' named entity 'FOURTEEN' named entity 'LINEAR REGRESSION ANALYSIS' named entity 'REVERSE-TRANSCRIPTION' named entity 'RNA' named entity 'CLINICAL' named entity 'TESTED' named entity 'PROVIDES' named entity 'BUT' named entity 'ASSAY' named entity 'PRIMERS' named entity 'GENE' named entity 'SIMPLE' named entity 'LABORATORIES' named entity 'LIMIT OF DETECTION' named entity 'PERFORMANCE' named entity 'USING' named entity 'RESOURCE' named entity 'PATHOGENS' named entity 'SMALL INTESTINE' named entity 'RECOMBINASE' named entity 'AMPLIFICATION' named entity 'DETECTION' named entity 'PROBE' named entity 'ALTERNATIVE' named entity 'CONSERVED' named entity 'RAPID' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'REVERSE TRANSCRIPTION' named entity 'SAMPLES' named entity '100%' named entity 'REAL-TIME RT-PCR' named entity 'TO DETECT' named entity 'RECOMBINASE' named entity '40%' named entity 'EVALUATED' named entity 'REAL-TIME' named entity 'USING' named entity 'POLYMERASE' named entity 'SITE' named entity 'AGREEMENT' named entity 'DETECTION' named entity 'DETECT' named entity 'USEFUL' named entity 'SPECIFIC' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'DIAGNOSTIC' named entity 'POLYMERASE' named entity 'COPIES' named entity 'FACILITIES' named entity 'ABSENT' named entity 'PORTION' named entity '28S' named entity 'TEMPLATE' named entity 'MIN' named entity 'VALUE' named entity 'REACTION' named entity 'RELATED' named entity 'STUDY' named entity 'PORCINE RESPIRATORY CORONAVIRUS' named entity 'RAPID'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software