About: Determination of sialic acids in immune system cells (coelomocytes) of sea urchin, Paracentrotus lividus, using capillary LC-ESI-MS/MS

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About: Determination of sialic acids in immune system cells (coelomocytes) of sea urchin, Paracentrotus lividus, using capillary LC-ESI-MS/MS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Determination of sialic acids in immune system cells (coelomocytes) of sea urchin, Paracentrotus lividus, using capillary LC-ESI-MS/MS
Creator	Deveci, Remziye Izzeto, Savaş
Source	Elsevier; Medline; PMC
abstract	Coelomocytes are considered to be immune effectors of sea urchins. Coelomocytes are the freely circulating cells in the body fluid contained in echinoderm coelom and mediate the cellular defence responses to immune challenges by phagocytosis, encapsulation, cytotoxicity and the production of antimicrobial agents. Coelomocytes have the ability to recognize self from non-self. Considering that sialic acids play important roles in immunity, we determined the presence of sialic acid types in coelomocytes of Paracentrotus lividus. Homogenized coelomocytes were kept in 2 M aqueous acetic acid at 80 °C for 3 h to liberate sialic acids. Sialic acids were determined by derivatization with 1,2-diamino-4,5-methylenediaoxy-benzene dihydrochloride (DMB) followed by capillary liquid-chromatography-electrospray ionization/tandem mass spectrometry (CapLC-ESI-MS/MS). Standard sialic acids; Neu5Ac, Neu5Gc, KDN and bovine submaxillary mucin showing a variety of sialic acids were used to confirm sialic acids types. We found ten different types of sialic acids (Neu5Gc, Neu5Ac, Neu5Gc9Ac, Neu5Gc8Ac, Neu5,9Ac(2), Neu5,7Ac(2), Neu5,8Ac(2), Neu5,7,9Ac(3), Neu5Gc7,9Ac(2), Neu5Gc7Ac) isolated in limited amounts from total coelomocyte population. Neu5Gc type of sialic acids in coelomocytes was the most abundant type sialic acid when compared with other types. This is the first report on the presence of sialic acid types in coelomocytes of P. lividus using CapLC-ESI-MS/MS-Ion Trap system (Capillary Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry).
has issue date	2013-11-09(xsd:dateTime)
bibo:doi	10.1016/j.fsi.2013.10.029
bibo:pmid	24215912
has license	no-cc
sha1sum (hex)	496b549a2327f4d55ee0f0efc2085516b8e79e6b
schema:url	https://doi.org/10.1016/j.fsi.2013.10.029
resource representing a document's title	Determination of sialic acids in immune system cells (coelomocytes) of sea urchin, Paracentrotus lividus, using capillary LC-ESI-MS/MS
has PubMed Central identifier	PMC7111654
has PubMed identifier	24215912
schema:publication	Fish Shellfish Immunol
resource representing a document's body	covid:496b549a2327f4d55ee0f0efc2085516b8e79e6b#body_text
is schema:about of	named entity 'immunity' named entity 'cellular' named entity 'body fluid' named entity 'mediate' named entity 'Neu5Ac' named entity 'acid' named entity 'IMMUNE SYSTEM' named entity 'BODY FLUID' named entity 'TYPE' named entity 'USED' named entity 'TOTAL' named entity 'AMOUNTS' named entity 'FOUND' named entity 'RESPONSES' named entity 'COMPARED' named entity 'ABUNDANT' named entity 'NEU5AC' named entity 'COELOMOCYTE' named entity 'DETERMINED BY' named entity 'STANDARD' covid:arg/496b549a2327f4d55ee0f0efc2085516b8e79e6b named entity 'HAVE' named entity 'IONIZATION' named entity 'CHALLENGES' named entity 'LIMITED' named entity 'AQUEOUS' named entity 'PARACENTROTUS LIVIDUS' named entity 'USING' named entity 'USING' named entity 'IMMUNE' named entity 'IMPORTANT' named entity 'LIQUID' named entity 'ISOLATED' named entity 'SEA URCHIN' named entity 'ESI-MS' named entity 'VARIETY' named entity 'FOLLOWED BY' named entity 'FREELY' named entity 'PRESENCE OF' named entity 'ANTIMICROBIAL AGENTS' named entity 'KDN' named entity 'DETERMINED' named entity 'REPORT' named entity 'CAPLC' named entity 'ENCAPSULATION' named entity 'ROLES' named entity 'BENZENE' named entity 'MEDIATE' named entity 'PLAY' named entity 'CELLS' named entity 'LC-ESI-MS' named entity 'SIALIC ACIDS' named entity 'MUCIN' named entity 'CAPILLARY' named entity 'BOVINE' named entity 'SUBMAXILLARY' named entity 'ACETIC ACID' named entity 'DIFFERENT' named entity 'SYSTEM' named entity 'COELOM' named entity 'NON-SELF' named entity 'CONSIDERED' named entity 'ELECTROSPRAY IONIZATION' named entity 'POPULATION' named entity 'PRODUCTION' named entity 'CONFIRM' named entity 'IMMUNITY' named entity '3 H' named entity 'ECHINODERM' named entity 'DERIVATIZATION' named entity 'LIBERATE' named entity 'ION TRAP' named entity 'SEA URCHINS' named entity 'CELLULAR' named entity 'CYTOTOXICITY' named entity 'LIQUID CHROMATOGRAPHY' named entity 'CIRCULATING CELLS' named entity 'SIALIC ACIDS' named entity 'NEU5GC' named entity 'TYPES'

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