About: Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus

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About: Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus
Creator	Parida, Manmohan Tomar, Shailly Saha, Amrita Kesari, Pooja Narwal, Manju Priya, Raj Shrivastava, Ambuj Bhagyawant, Sameer Dash, Paban Tripathi, Nagesh Kameswara Rao, M Narayan Acharya, Badri
Source	Medline; PMC
abstract	Chikungunya virus has emerged as one of the most important global arboviral threats over the last decade. Inspite of large scale morbidity, with long lasting polyarthralgia, so far no licensed vaccine or antiviral is available. CHIKV nsP2 protease is crucial for processing of viral nonstructural polypeptide precursor to release enzymes required for viral replication, thus making it a promising drug target. In this study, high cell density cultivation (HCDC) of Escherichia coli in batch process was carried out to produce rCHIKV nsP2pro in a cost-effective manner. The purified nsP2pro and fluorogenic peptide substrate have been adapted for fluorescence resonance energy transfer (FRET) based high throughput screening (HTS) assay with Z’ value and CV of 0.67 ± 0.054 and <10% respectively. We used this cell free HTS system to screen panel of metal ions and its conjugate which revealed zinc acetate as a potential candidate, which was further found to inhibit CHIKV in Vero cells. Scale-up process has not been previously reported for any of the arboviral nonstructural enzymes. The successful scale-up method for viral protease together with a HTS assay could lead to the development of industrial level large-scale screening platform for identification of protease inhibitors against emerging and re-emerging viruses.
has issue date	2018-07-17(xsd:dateTime)
bibo:doi	10.1038/s41598-018-29024-2
bibo:pmid	30018455
has license	cc-by
sha1sum (hex)	49e38057aa849ee6e304090b6ee1d2633b76f274
schema:url	https://doi.org/10.1038/s41598-018-29024-2
resource representing a document's title	Development of nsP2 protease based cell free high throughput screening assay for evaluation of inhibitors against emerging Chikungunya virus
has PubMed Central identifier	PMC6050329
has PubMed identifier	30018455
schema:publication	Sci Rep
resource representing a document's body	covid:49e38057aa849ee6e304090b6ee1d2633b76f274#body_text
is schema:about of	named entity 'level' named entity 'based' named entity 'drug target' named entity 'industrial' named entity 'arboviral' named entity 'process' named entity 'purified' named entity 'HTS' named entity 'conjugate' named entity 'high throughput screening' named entity 'OPEN' named entity 'HIGH THROUGHPUT SCREENING' named entity 'CHIKUNGUNYA VIRUS' named entity 'TO INHIBIT' named entity 'AVAILABLE' named entity 'IMPORTANT' named entity 'MORBIDITY' named entity 'CELL FREE' named entity 'VIRUSES' named entity 'ASSAY' named entity 'CULTIVATION' named entity 'PEPTIDE' named entity 'DECADE' named entity 'LARGE SCALE' named entity 'SCREEN' named entity 'VIRAL REPLICATION' named entity 'ITS' named entity 'VALUE' named entity 'BASED' named entity 'ZINC ACETATE' named entity 'ESCHERICHIA COLI' named entity 'PRECURSOR' named entity 'FLUORESCENCE RESONANCE ENERGY TRANSFER ' named entity 'SCALE' named entity 'HIGH CELL DENSITY' named entity 'VERO CELLS' named entity 'PROCESS' named entity 'VIRAL' named entity 'RELEASE' named entity 'USED' named entity 'IDENTIFICATION' named entity 'REQUIRED' named entity 'HAVE' named entity 'SYSTEM' named entity 'PROTEASE INHIBITORS' named entity 'DRUG TARGET' named entity 'NSP2' named entity 'ANTIVIRAL' named entity 'METAL IONS' named entity 'FOUND' named entity 'ONE OF' named entity 'PROCESSING' named entity '0.67' named entity 'LICENSED VACCINE' named entity 'ENZYMES' named entity 'HTS' named entity 'PLATFORM' named entity 'TO PRODUCE' named entity 'LEVEL' named entity 'SUCCESSFUL' named entity 'DEVELOPMENT' named entity 'ASSAY' named entity 'CELL FREE' named entity 'DEVELOPMENT' named entity 'CHIKUNGUNYA VIRUS' named entity 'STUDY' named entity 'LARGE' named entity 'CARRIED' named entity 'HIGH THROUGHPUT SCREENING' named entity 'EVALUATION'

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