About: Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes

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About: Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes
Creator	Zhang, Li Chen, Ting Sun, Yang Xu, Qiang
Source	Elsevier; Medline; PMC
abstract	In the present study, we performed to selectively deplete glycyrrhizin from Si-Ni-San, a traditional Chinese prescription that consists of 4 Chinese herbs including Radix Glycyrrhizae Uralensis, and examined its influence on the suppressing activity of Si-Ni-San against contact sensitivity in mice. An immunoaffinity column was made by covalently coupling the polyclonal antibody, obtained by the immunization with glycyrrhizin–BSA conjugate, to CNBr-activated Sepharose 4B. By using this column, glycyrrhizin in Si-Ni-San was selectively and almost completely depleted from the whole extract, which was confirmed by high-performance liquid chromatography (HPLC). Both 200 mg/kg of Si-Ni-San and 10 mg/kg of glycyrrhizin, the dose corresponding to its proportion contained in Si-Ni-San, significantly reduced the ear swelling of picryl chloride (PCl)-induced ear contact sensitivity in mice and the inhibition by Si-Ni-San was stronger than that by glycyrrhizin. The adhesion activity to type IV collagen of the isolated spleen cells from PCl-sensitized mice was significantly decreased by both Si-Ni-San and glycyrrhizin. However, the glycyrrhizin-depleted sample of Si-Ni-San (Si-Ni-San(GL−)) only showed a slight inhibition on the cell adhesion. Furthermore, the spleen cells from PCl-sensitized mice produced more matrix metalloproteinase (MMP)-2 and -9 than naive spleen cells did, and both Si-Ni-San and glycyrrhizin remarkably reduced MMP-2 and MMP-9 production. In contrast, Si-Ni-San(GL−) only showed a slight inhibition. These results suggest that glycyrrhizin may act as one of the active constituents of Si-Ni-San in inhibiting delayed-type hypersensitivity reaction via down-regulating the MMP production and the cell adhesion to extracellular matrix. The present study also provides a new approach to recognize and validate an active constituent in traditional prescription through a selective depletion.
has issue date	2005-03-21(xsd:dateTime)
bibo:doi	10.1016/j.intimp.2005.02.015
bibo:pmid	15914324
has license	no-cc
sha1sum (hex)	4a55240b7aba7aa52868e95c4afedf215d9f21df
schema:url	https://doi.org/10.1016/j.intimp.2005.02.015
resource representing a document's title	Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes
has PubMed Central identifier	PMC7106220
has PubMed identifier	15914324
schema:publication	Int Immunopharmacol
resource representing a document's body	covid:4a55240b7aba7aa52868e95c4afedf215d9f21df#body_text
is schema:about of	named entity 'TRADITIONAL' named entity 'T LYMPHOCYTES' named entity 'ALMOST' named entity 'CONFIRMED BY' named entity 'PCL' named entity 'HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'PROVIDES' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'SELECTIVE' named entity 'GLYCYRRHIZIN' named entity 'DEPLETION' named entity 'APPROACH' named entity 'SUPPRESSING' named entity 'ACTIVE CONSTITUENT' named entity 'COMPLETELY' named entity 'MMP' named entity 'MICE' named entity 'EAR SWELLING' named entity 'SUGGEST' named entity 'DOSE' named entity 'TYPE IV COLLAGEN' named entity 'PROPORTION' named entity 'IMMUNOAFFINITY' named entity 'DID' named entity 'RECOGNIZE' named entity 'ACT' named entity 'SI-NI-SAN' named entity 'INHIBITING' named entity 'COUPLING' named entity 'PERFORMED' named entity 'BLOCKS' named entity 'CHINESE' named entity 'ONE OF' named entity 'CELL ADHESION' named entity 'SPLEEN' named entity 'RADIX' named entity '200' named entity 'PRODUCTION' named entity 'MMP-9' named entity 'ITS' named entity 'INFLUENCE' named entity 'IMMUNIZATION' named entity 'SI-NI-SAN' named entity 'ITS' named entity 'DEPLETION' named entity 'RECOVERS' named entity 'GLYCYRRHIZIN' named entity 'METALLOPROTEINASES' named entity 'MICE' named entity 'CONTACT SENSITIVITY' named entity 'ADHESION' named entity 'CHINESE HERBS' named entity 'CNBR' named entity 'EXAMINED' named entity 'REGULATING' named entity 'SAN' named entity 'CONTRAST' named entity 'HYPERSENSITIVITY REACTION' named entity 'DELAYED-TYPE HYPERSENSITIVITY' named entity 'DECREASED BY' named entity 'SAMPLE' named entity 'REDUCED' named entity 'ACTIVE' named entity 'PICRYL CHLORIDE' named entity 'MG%' named entity 'STUDY' named entity 'EXTRACELLULAR MATRIX' named entity 'CELLS' named entity 'RESULTS' named entity 'EFFECT' named entity 'CONSTITUENTS' named entity 'SLIGHT' named entity 'ADHESION' named entity 'EXTRACT' named entity 'ACTIVATED' named entity 'ACTIVITY' named entity 'DEPLETED' named entity 'NI-'

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