About: Construction of a CaHPO(4)-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-d-glucuronide preparation

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About: Construction of a CaHPO(4)-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-d-glucuronide preparation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Construction of a CaHPO(4)-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-d-glucuronide preparation
Creator	Li, Chun Feng, Xudong Jiang, Tian Hou, Yuhui Zhang, Tengjiang
source	PMC
abstract	Glycyrrhetinic acid 3-O-mono-β-d-glucuronide (GAMG), an important pharmaceutical intermediate and functional sweetener, has broad applications in the food and medical industries. A green and cost-effective method for its preparation is highly desired. Using site-directed mutagenesis, we previously obtained a variant of β-glucuronidase from Aspergillus oryzae Li-3 (PGUS1), which can specifically transform glycyrrhizin (GL) into GAMG. In this study, a facile method was established to prepare a CaHPO(4)-PGUS1 hybrid nanoflower for enzyme immobilization, based on protein-inorganic hybrid self-assembly. Under optimal conditions, 1.2 mg of a CaHPO(4)-PGUS1 hybrid nanoflower precipitate with 71.2% immobilization efficiency, 35.60 mg·g(−1) loading capacity, and 118% relative activity was obtained. Confocal laser scanning microscope and scanning electron microscope results showed that the enzyme was encapsulated in the CaHPO(4)-PGUS1 hybrid nanoflower. Moreover, the thermostability of the CaHPO(4)-PGUS1 hybrid nanoflower at 55°C was improved, and its half-life increased by 1.3 folds. Additionally, the CaHPO(4)-PGUS1 hybrid nanoflower was used for the preparation of GAMG through GL hydrolysis, with the conversion rate of 92% in 8 h, and after eight consecutive runs, it had 60% of its original activity.
has issue date	2019-07-20(xsd:dateTime)
bibo:doi	10.1007/s11705-019-1834-z
has license	no-cc
sha1sum (hex)	4b05b1f7307f3c29da92257cc122b106d83a9809
schema:url	https://doi.org/10.1007/s11705-019-1834-z
resource representing a document's title	Construction of a CaHPO(4)-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-d-glucuronide preparation
has PubMed Central identifier	PMC7089396
schema:publication	Front Chem Sci Eng
resource representing a document's body	covid:4b05b1f7307f3c29da92257cc122b106d83a9809#body_text
is schema:about of	named entity 'conditions' named entity 'glycyrrhizin' named entity 'highly' named entity 'HYBRID' named entity '1.3' named entity 'CONVERSION' named entity 'IMMOBILIZATION' named entity 'METHOD' named entity 'ENZYME' named entity 'USED' named entity 'ESTABLISHED' named entity 'LOADING' named entity 'HYDROLYSIS' named entity 'FOOD' named entity 'MEDICAL' named entity 'USING' named entity '60%' named entity 'RELATIVE ACTIVITY' named entity 'TO PREPARE' named entity 'SCANNING ELECTRON MICROSCOPE' named entity 'CONSECUTIVE' named entity 'HYBRID' named entity 'GAMG' named entity 'PRECIPITATE' named entity 'BASED' named entity 'ACTIVITY' named entity 'INTERMEDIATE' named entity 'CONFOCAL LASER SCANNING MICROSCOPE' named entity 'CONDITIONS' named entity 'APPLICATIONS' named entity 'STUDY' named entity 'CONSTRUCTION' named entity 'EFFICIENCY' covid:arg/4b05b1f7307f3c29da92257cc122b106d83a9809 named entity 'INORGANIC' named entity 'INCREASED' named entity '118' named entity 'INDUSTRIES' named entity 'OBTAINED' named entity 'VARIANT' named entity 'ENZYME IMMOBILIZATION' named entity 'ORIGINAL' named entity 'GLUCURONIDASE' named entity 'MG%' named entity 'GLYCYRRHETINIC ACID' named entity 'MONO' named entity 'APPLICATION' named entity 'ASPERGILLUS ORYZAE' named entity 'GLYCYRRHIZIN' named entity 'PROTEIN' named entity 'PREPARATION' named entity 'GLUCURONIDE' named entity 'SELF-ASSEMBLY' named entity 'PREVIOUSLY' named entity 'THERMOSTABILITY' named entity 'FUNCTIONAL' named entity 'RESULTS' named entity 'RUNS' named entity 'TRANSFORM' named entity 'EFFECTIVE' named entity 'GREEN ' named entity 'MONO' named entity 'FOLDS' named entity 'ENCAPSULATED' named entity 'GLUCURONIDE' named entity 'CAPACITY' named entity 'ITS' named entity 'COST' named entity 'PROTEIN' named entity 'ITS' named entity 'PHARMACEUTICAL' named entity 'PREPARATION' named entity 'HIGHLY' named entity 'IMPROVED' named entity 'INORGANIC' named entity 'MUTAGENESIS' named entity 'RATE' named entity '24G' named entity '1.2'

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