About: Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

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About: Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species
Creator	Nagy, Alexander Vilcek, Stefan Cernikova, Lenka Dan, Adam Dirbakova, Zuzana Havlickova, Martina Hornickova, Jitka Jirincova, Helena Mojzis, Miroslav Pirchanova, Zuzana Ursu, Krisztina Vostinakova, Veronika
Source	Medline; PMC
abstract	The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-010-0636-x) contains supplementary material, which is available to authorized users.
has issue date	2010-03-13(xsd:dateTime)
bibo:doi	10.1007/s00705-010-0636-x
bibo:pmid	20229116
has license	no-cc
sha1sum (hex)	4b1ba87175820f0eab886f0b291a0494782918d9
schema:url	https://doi.org/10.1007/s00705-010-0636-x
resource representing a document's title	Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species
has PubMed Central identifier	PMC7086820
has PubMed identifier	20229116
schema:publication	Arch Virol
resource representing a document's body	covid:4b1ba87175820f0eab886f0b291a0494782918d9#body_text
is schema:about of	named entity 'VIRUSES' named entity 'strains' named entity 'one-step' named entity 'Development' named entity 'TESTED' named entity 'SELECTED' named entity 'STRAINS' named entity 'EXPERIMENTAL' named entity 'LABORATORIES' named entity 'PANDEMIC' named entity 'EVALUATE' named entity 'BIOINFORMATICS ANALYSIS' named entity 'ESTIMATED' covid:arg/4b1ba87175820f0eab886f0b291a0494782918d9 named entity 'AUGUST' named entity 'KNOWN' named entity '20%' named entity '10%' named entity 'APPROXIMATELY' named entity 'CONSERVED' named entity 'EXCELLENT' named entity 'PROTEIN ' named entity 'PCR ASSAY' named entity 'VIRUS SEQUENCE' named entity 'MODIFIED' named entity 'REGIONS' named entity 'TO DETECT' named entity 'REACTION' named entity 'BIOINFORMATIC' named entity 'LNA' named entity 'COMPREHENSIVE' named entity 'PRIMERS' named entity 'RESULTS' named entity 'PREDICTED' named entity 'HUMAN' named entity 'LEVEL' named entity 'REFERENCE' named entity 'SPECIES' named entity 'MAMMAL' named entity 'DEVELOPMENT' named entity 'STEP' named entity 'AVIAN' named entity 'DETECTION' named entity 'INFLUENZA A' named entity 'REAL-TIME RT-PCR' named entity 'PARAMETERS' named entity 'MEMBRANE PROTEIN' named entity 'VIRUS' named entity '100%' named entity 'TARGET' named entity 'IDENTIFIED BY' named entity 'ORIGIN' named entity 'COPIES' named entity 'INFLUENZA A' named entity 'SEGMENT' named entity 'SWINE' named entity 'INFLUENZA VIRUS' named entity 'RRT-PCR' named entity 'DETECTION LIMIT' named entity 'SEQUENCE DATABASE' named entity 'ASSAY' named entity 'TAQMAN' named entity 'EXTREMELY' named entity 'DETECTION' named entity 'EQUINE' named entity 'SEQUENCES' named entity 'PCR' named entity 'INCLUDING' named entity 'VIRUSES' named entity 'UNIVERSAL'

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