About: Development of a RT Real-Time PCR for the Detection and Quantification of Human Rhinoviruses

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a RT Real-Time PCR for the Detection and Quantification of Human Rhinoviruses
Creator	Cavallo, Rossana Costa, Cristina Ae, Bergallo Ae, Elia Ae, Mantovani Ae, Stefano Gruosso, Valentina Mariateresa, A Massimiliano, A Samantha, A Sidoti, Francesca
Source	Medline; PMC
abstract	Human Rhinoviruses (HRV) are the most common viral agents, being responsible for upper as well as lower respiratory tract infections. Evidence demonstrating that HRV disease is not exclusively limited to the upper airways and may cause lower respiratory complications, together with the frequency of HRV infections and the increasing number of immunocompromised patients underline the need for including HRV in virological diagnostics of acute lower respiratory tract illness. This article describes the development and optimization of a reverse transcription (RT) real-time PCR assay for quantification of HRV RNA in clinical samples. Efficiency, sensitivity, specificity, inter- and intra-assay variability, and dynamic range have been determined. Subsequently, the assay has been validated on bronchoalveolar lavage (BAL) specimens obtained from immunocompetent and immunocompromised patients.
has issue date	2009-03-17(xsd:dateTime)
bibo:doi	10.1007/s12033-009-9164-x
bibo:pmid	19291427
has license	no-cc
sha1sum (hex)	4c343dfb224188a4f09a63f7fb53a6a1c14ce213
schema:url	https://doi.org/10.1007/s12033-009-9164-x
resource representing a document's title	Development of a RT Real-Time PCR for the Detection and Quantification of Human Rhinoviruses
has PubMed Central identifier	PMC7091102
has PubMed identifier	19291427
schema:publication	Mol Biotechnol
resource representing a document's body	covid:4c343dfb224188a4f09a63f7fb53a6a1c14ce213#body_text
is schema:about of	named entity 'DEVELOPMENT' named entity 'acute' named entity 'immunocompetent' named entity 'Efficiency' named entity 'responsible' named entity 'sensitivity' named entity 'DETECTION' named entity 'HUMAN' named entity 'DEVELOPMENT' named entity 'SENSITIVITY' named entity 'RNA' named entity 'RESPONSIBLE' named entity 'DYNAMIC RANGE' named entity 'DETERMINED' named entity 'FREQUENCY' named entity 'IMMUNOCOMPETENT' named entity 'ASSAY' named entity 'INCREASING' named entity 'BEING' named entity 'VALIDATED' named entity 'REALTIME' named entity 'NUMBER OF' named entity 'QUANTIFICATION' named entity 'REAL-TIME PCR ' named entity 'HUMAN' named entity 'RHINOVIRUSES' named entity 'VARIABILITY' named entity 'EFFICIENCY' named entity 'INTER-' named entity 'INFECTIONS' named entity 'SAMPLES' named entity 'DISEASE' named entity 'COMMON' named entity 'REVERSE TRANSCRIPTION' named entity 'QUANTIFICATION' named entity 'VIROLOGICAL' named entity 'LOWER RESPIRATORY TRACT' named entity 'IMMUNOCOMPROMISED PATIENTS' named entity 'EVIDENCE' named entity 'RHINOVIRUSES' named entity 'LIMITED' named entity 'SPECIMENS' named entity 'LOWER' named entity 'CLINICAL' named entity 'HAVE' named entity 'HRV' named entity 'INCLUDING' named entity 'OPTIMIZATION' named entity 'ACUTE' named entity 'CAUSE' named entity 'PCR ASSAY' named entity 'NEED FOR' named entity 'AIRWAYS' named entity 'DIAGNOSTICS' named entity 'ILLNESS' named entity 'BRONCHOALVEOLAR LAVAGE' named entity 'OBTAINED' named entity 'RESPIRATORY COMPLICATIONS' named entity 'VIRAL' named entity 'SPECIFICITY' named entity 'LOWER RESPIRATORY TRACT INFECTIONS' named entity 'ARTICLE' named entity 'UPPER' named entity 'reverse transcription' named entity 'determined' named entity 'assay' named entity 'limited' named entity 'assay' named entity 'underline' named entity 'upper airways' named entity 'patients'

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