About: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis

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About: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
Creator	Pantchev, Nikola Hartmann, Katrin Balzer, Hans-Joerg Felten, Sandra Matiasek, Kaspar Leutenegger, Christian Emmler, Laura
Source	Medline; PMC
abstract	OBJECTIVES: Feline infectious peritonitis (FIP) emerges when feline coronaviruses (FCoVs) mutate within their host to a highly virulent biotype and the immune response is not able to control the infection. FCoV spike (S) gene mutations are considered to contribute to the change in virulence by enabling FCoV infection of and replication in macrophages. This study investigated the presence of FCoV with and without S gene mutations in cats with FIP using two different real-time RT-PCRs on different samples obtained under clinical conditions. METHODS: Fine-needle aspirates (FNAs) and incisional biopsies (IBs) of popliteal and mesenteric lymph nodes, liver, spleen, omentum and kidneys (each n = 20), EDTA blood (n = 13), buffy coat smears (n = 13), serum (n = 11), effusion (n = 14), cerebrospinal fluid (n = 16), aqueous humour (n = 20) and peritoneal lavage (n = 6) were obtained from 20 cats with FIP diagnosed by immunohistochemistry. Samples were examined by RT-PCR targeting the FCoV 7b gene, detecting all FCoV, and S gene mutation RT-PCR targeting mutations in nucleotides 23531 and 23537. The prevalence of FCoV detected in each sample type was calculated. RESULTS: In 20/20 cats, FCoV with S gene mutations was present in at least one sample, but there was variation in which sample was positive. FCoV with mutations in the S gene were most frequently found in effusion (64%, 95% confidence interval [CI] 39–89), followed by spleen, omentum and kidney IBs (50%, 95% CI 28–72), mesenteric lymph node IBs and FNAs (45%, 95% CI 23–67), and FNAs of spleen and liver and liver IBs (40%, 95% CI 19–62). CONCLUSIONS AND RELEVANCE: In these 20 cats with FIP, FCoVs with S gene mutations were found in every cat in at least one tissue or fluid sample. This highlights the association between mutated S gene and systemic FCoV spread. Examining a combination of different samples increased the probability of finding FCoV with the mutated S gene.
has issue date	2019-11-15(xsd:dateTime)
bibo:doi	10.1177/1098612x19886671
bibo:pmid	31729897
has license	cc-by
sha1sum (hex)	4cc8f05660417afe64bbb507b292202b263328f5
schema:url	https://doi.org/10.1177/1098612x19886671
resource representing a document's title	Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
has PubMed Central identifier	PMC7206566
has PubMed identifier	31729897
schema:publication	J Feline Med Surg
resource representing a document's body	covid:4cc8f05660417afe64bbb507b292202b263328f5#body_text
is schema:about of	named entity 'mesenteric' named entity 'Objectives' named entity 'aspirates' named entity 'immune response' named entity 'omentum' named entity 'calculated' named entity 'feline' named entity 'liver' named entity 'immunohistochemistry' named entity 'gene' named entity 'CHANGE IN' named entity '23537' named entity 'SPIKE' named entity 'PRESENCE OF' named entity 'INFECTION' named entity 'GENE MUTATIONS' named entity 'SAMPLE TYPE' named entity 'CEREBROSPINAL FLUID' named entity 'FELINE CORONAVIRUS' named entity 'macrophages' named entity 'aqueous humour' named entity 'emerges' named entity 'coronaviruses' named entity 'conditions' named entity '20/20' named entity 'biopsies' named entity 'blood' named entity 'cats' named entity 'prevalence' named entity 'detected' named entity 'gene' named entity 'nucleotides' named entity 'omentum' named entity 'host' named entity 'biopsies' named entity 'infection' named entity 'lymph node' named entity 'FIP' named entity 'cerebrospinal fluid' named entity 'omentum' named entity 'gene mutations' named entity '20/20' named entity 'macrophages' named entity 'aqueous humour' named entity 'kidneys' named entity 'RT-PCR' named entity 'immune response' named entity 'FIP' named entity 'Methods' named entity 'FCoV' named entity 'histopathology' named entity 'viral genome' named entity 'amino acid' named entity 'FIP' named entity 'viral cell entry' named entity 'viral RNA' named entity 'mutation' named entity 'virus' named entity 'FCoV' named entity 'gene' named entity 'RT-PCR' named entity 'antigen' named entity 'immunostaining' named entity 'RT-PCR' named entity 'gene mutation' named entity 'gene' named entity 'serum' named entity 'gene mutation' named entity 'pathogenesis' named entity 'FCoV' named entity 'gene mutations' named entity 'genome sequences' named entity 'gene mutations' named entity 'FIP' named entity 'FIP'

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