About: Cloning and expression of two fragments of the S gene of canine coronavirus type I

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About: Cloning and expression of two fragments of the S gene of canine coronavirus type I Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning and expression of two fragments of the S gene of canine coronavirus type I
Creator	Buonavoglia, Canio Decaro, Nicola Elia, Gabriella Martella, Vito Tempesta, Maria Tinelli, Antonella Pratelli, Annamaria Rocca, Stefano Tola, Sebastiana
Source	Elsevier; Medline; PMC
abstract	Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of CCoV type I antibodies in dog sera. Four canine sera-positive for CCoV type II, four sera-positive for CCoV type I and 10 negative control sera were examined. Only the sera-positive for CCoV type I strongly reacted with both the proteins, whereas the sera-positive for CCoV type II showed low reactivity in the ELISA test. As CCoV type I seems to be not cultivable in cell cultures, the recombinant fragments of the S protein represent a unique method to study, preliminarily, the immunological and the pathogenetic characteristics of this new virus.
has issue date	2004-02-03(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2003.12.003
bibo:pmid	15019260
has license	no-cc
sha1sum (hex)	4d24eb08456d9723ba8c90fdf6dc75f5fdfeb4a9
schema:url	https://doi.org/10.1016/j.jviromet.2003.12.003
resource representing a document's title	Cloning and expression of two fragments of the S gene of canine coronavirus type I
has PubMed Central identifier	PMC7119755
has PubMed identifier	15019260
schema:publication	J Virol Methods
resource representing a document's body	covid:4d24eb08456d9723ba8c90fdf6dc75f5fdfeb4a9#body_text
is schema:about of	named entity 'recombinant' named entity 'FRAGMENTS' named entity 'METHOD' named entity 'CHARACTERISTICS' named entity 'NEW' named entity 'ELISA' named entity 'POSITIVE FOR' named entity 'TYPE II' named entity 'LENGTH' named entity 'STUDY' named entity 'CELL CULTURES' named entity 'TYPE I' named entity 'CANINE CORONAVIRUS' named entity 'DOG' named entity 'S66' named entity 'AMINO ACIDS' named entity 'RECOMBINANT' named entity 'REPRESENT' named entity 'PROTEINS' named entity 'S55' named entity 'CLONING' named entity 'CANINE' named entity '350' named entity 'EXAMINED' named entity 'UNIQUE' named entity 'LOW' named entity 'VIRUS' named entity 'REACTIVITY' named entity 'IDENTIFIED' covid:arg/4d24eb08456d9723ba8c90fdf6dc75f5fdfeb4a9 named entity 'DETECTION' named entity 'GLYCOPROTEIN' named entity 'TYPE I' named entity 'EXPRESSION' named entity 'CANINE CORONAVIRUS' named entity 'NEGATIVE CONTROL' named entity 'SERA' named entity 'NEWLY' named entity 'S PROTEIN' named entity 'RECOMBINANT PROTEINS' named entity 'ANTIBODIES' named entity 'TEST' named entity 'EXPRESSED' named entity 'FRAGMENTS' named entity 'SYSTEM' named entity 'IMMUNOLOGICAL' named entity 'EMPLOYED' named entity 'PROTEIN ' named entity 'PURIFIED' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'GENE' named entity 'type II' named entity 'recombinant proteins' named entity 'amino acids' named entity 'cell cultures' named entity 'type I' named entity 'type I' named entity 'glycoprotein' named entity 'recombinant proteins' named entity 'cell cultures' named entity 'virus' named entity 'pathogenetic' named entity 'enzyme-linked immunosorbent assay' named entity 'coronavirus' named entity 'immunological' named entity 'negative control' named entity 'protein' named entity 'ELISA test' named entity 'dog' named entity 'antibodies' named entity 'recombinant' named entity 'amino acids' named entity 'canine coronavirus'

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