About: Identification of novel RNA secondary structures within the hepatitis C virus genome reveals a cooperative involvement in genome packaging

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of novel RNA secondary structures within the hepatitis C virus genome reveals a cooperative involvement in genome packaging
Creator	Bingham, R Dykeman, E Harris, & Stewart, H Stockley, P Tuplin, A Twarock, R White, S Zothner, C
source	Medline; PMC
abstract	The specific packaging of the hepatitis C virus (HCV) genome is hypothesised to be driven by Core-RNA interactions. To identify the regions of the viral genome involved in this process, we used SELEX (systematic evolution of ligands by exponential enrichment) to identify RNA aptamers which bind specifically to Core in vitro. Comparison of these aptamers to multiple HCV genomes revealed the presence of a conserved terminal loop motif within short RNA stem-loop structures. We postulated that interactions of these motifs, as well as sub-motifs which were present in HCV genomes at statistically significant levels, with the Core protein may drive virion assembly. We mutated 8 of these predicted motifs within the HCV infectious molecular clone JFH-1, thereby producing a range of mutant viruses predicted to possess altered RNA secondary structures. RNA replication and viral titre were unaltered in viruses possessing only one mutated structure. However, infectivity titres were decreased in viruses possessing a higher number of mutated regions. This work thus identified multiple novel RNA motifs which appear to contribute to genome packaging. We suggest that these structures act as cooperative packaging signals to drive specific RNA encapsidation during HCV assembly.
has issue date	2016-03-14(xsd:dateTime)
bibo:doi	10.1038/srep22952
bibo:pmid	26972799
has license	cc-by
sha1sum (hex)	4e6e76a1940fbfadea1d88bbcbfe4f1554f7719b
schema:url	https://doi.org/10.1038/srep22952
resource representing a document's title	Identification of novel RNA secondary structures within the hepatitis C virus genome reveals a cooperative involvement in genome packaging
has PubMed Central identifier	PMC4789732
has PubMed identifier	26972799
schema:publication	Sci Rep
resource representing a document's body	covid:4e6e76a1940fbfadea1d88bbcbfe4f1554f7719b#body_text
is schema:about of	named entity 'ALTERED' named entity 'STEM-LOOP' named entity 'INTERACTIONS' named entity 'PRESENCE OF' named entity 'GENOMES' named entity 'SHORT' named entity 'HEPATITIS C VIRUS' named entity 'HIGHER' named entity 'GENOME' named entity 'CORE' named entity 'ENRICHMENT' named entity 'RANGE' named entity 'MOLECULAR' named entity 'INFECTIOUS' named entity 'SPECIFIC' named entity 'CORE PROTEIN' named entity 'RNA' named entity 'MUTATED' named entity 'POSSESS' named entity 'THESE' named entity 'protein' named entity 'mutated' named entity 'structures' named entity 'motifs' named entity 'aptamers' named entity 'multiple' named entity 'HCV' named entity 'terminal' named entity 'STRUCTURES' named entity 'NOVEL' named entity 'REVEALS' named entity 'ACT' named entity 'NUMBER OF' named entity 'PREDICTED' named entity 'INFECTIVITY' named entity 'VIRAL GENOME' named entity 'MOTIF' named entity 'SIGNALS' named entity 'PROTEIN ' named entity 'INVOLVED' named entity 'COMPARISON' named entity 'SELEX' named entity 'UNALTERED' named entity 'MOTIFS' named entity 'CLONE' named entity 'PRODUCING' named entity 'TO IDENTIFY' named entity 'HEPATITIS C VIRUS' named entity 'IDENTIFICATION' named entity 'INVOLVEMENT' named entity 'RNA' named entity 'PACKAGING' named entity 'GENOME' named entity 'SECONDARY' named entity 'NOVEL' named entity 'LEVELS' named entity 'APPEAR' named entity 'ENCAPSIDATION' named entity 'HCV' named entity 'DRIVE' named entity 'COOPERATIVE' named entity 'USED' named entity 'CONSERVED' named entity 'LIGANDS' named entity 'APTAMERS' named entity 'WORK' named entity 'TERMINAL LOOP' named entity 'VIRAL TITRE' named entity 'CONTRIBUTE ' named entity 'SUB-' named entity 'COOPERATIVE' named entity 'DRIVEN' named entity 'RNA REPLICATION'

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