About: Loop-mediated isothermal amplification establishment for detection of pseudorabies virus

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About: Loop-mediated isothermal amplification establishment for detection of pseudorabies virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Loop-mediated isothermal amplification establishment for detection of pseudorabies virus
Creator	En, Fang-Xue Jian, Li Qin, Chen Wei, Xiong
Source	Elsevier; Medline; PMC
abstract	A rapid, convenient and reliable pseudorabies virus (PRV) detection system was developed by using the loop-mediated isothermal amplification (LAMP) method. Six special primers were designed successfully based on the PRV DNA-binding protein (DBP) gene. The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h. The LAMP products had a ladder-like pattern of bands from 188 bp when electrophoresed on an agarose gel and its specificity was confirmed by digestion with Hinc II enzyme. Two naked-eye detection methods were developed for use in the field. The detection limit of the LAMP assay was found to be 10 fg DNA sample which was 100–1000-fold higher than that of PCR. By using DNA (or cDNA) samples extracted from three different PRV strains and six other viruses known to be related genetically to PRV or to cause similar clinical signals in pig, the system was identified to amplify only the PRV DNA. A comparison between the LAMP and PCR assay using five clinical samples showed good correlation.
has issue date	2008-05-12(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.03.028
bibo:pmid	18468699
has license	no-cc
sha1sum (hex)	4e7f6644a38aea6fd9938eb67122a71fbf6f8f3a
schema:url	https://doi.org/10.1016/j.jviromet.2008.03.028
resource representing a document's title	Loop-mediated isothermal amplification establishment for detection of pseudorabies virus
has PubMed Central identifier	PMC7112783
has PubMed identifier	18468699
schema:publication	J Virol Methods
resource representing a document's body	covid:4e7f6644a38aea6fd9938eb67122a71fbf6f8f3a#body_text
is schema:about of	named entity 'PSEUDORABIES VIRUS' named entity 'electrophoresed' named entity 'Two' named entity 'DNA' named entity 'assay' named entity 'LIKE' named entity 'PCR' named entity 'DETECTION METHODS' named entity 'RELIABLE' named entity 'AGAROSE GEL' named entity 'DNA' named entity 'LADDER' named entity 'IN THE FIELD' named entity '100' named entity '188' named entity 'PRIMERS' named entity '80%' named entity 'VIRUSES' named entity 'FOUND' named entity 'IDENTIFIED' named entity 'GENE' named entity 'PRV' named entity 'DETECTION' covid:arg/4e7f6644a38aea6fd9938eb67122a71fbf6f8f3a named entity 'ESTABLISHMENT' named entity 'CDNA' named entity 'ASSAY' named entity 'DETECTION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'SIMILAR' named entity 'COMPARISON' named entity 'HIGHER' named entity 'INCUBATION' named entity 'CONFIRMED BY' named entity 'SAMPLE' named entity 'LAMP' named entity 'GOOD' named entity 'DIFFERENT' named entity 'AMPLIFY' named entity 'USE' named entity 'EXTRACTED' named entity 'PCR ASSAY' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'ITS' named entity 'PIG' named entity 'USING' named entity 'RAPID' named entity 'METHOD' named entity 'PSEUDORABIES VIRUS' named entity 'EYE DETECTION' named entity 'DNA-BINDING PROTEIN' named entity 'DIGESTION' named entity 'BASED' named entity 'CORRELATION' named entity 'PROTEIN ' named entity 'CAUSE' named entity 'CONVENIENT' named entity 'PRODUCTS' named entity 'STRAINS' named entity 'SPECIAL' named entity 'FOLD' named entity 'KNOWN' named entity 'NAKED' named entity '1 H' named entity 'PATTERN' named entity 'SIGNALS' named entity '1000' named entity 'SPECIFICITY' named entity 'ENZYME' named entity 'DETECTION LIMIT' named entity 'BANDS' named entity 'RELATED' named entity 'SYSTEM' named entity 'SAMPLES' named entity 'CLINICAL' named entity 'LAMP' named entity 'products' named entity 'developed'

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