About: Viral interference between low pathogenic avian influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Viral interference between low pathogenic avian influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo
Creator	Ghram, Abdeljelil Laamiri, Nacira Aouini, Rim
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken eggs (ECE). Chicken embryo lung (CEL) and ECE were simultaneously or sequentially infected with IBV vaccine strain (H120) and LPAIV-H9N2 (A/Ck/TUN/145/2012) to evaluate viral interactionsin vitro and in ovo, respectively. Real-time RT-PCR was developed to specifically quantify both AIV and IBV genomes as well as viral gene copy numbers during mixed infections. The amount of IL-1 beta, in supernatants of co-infected cell cultures, was determined using an ELISA assay. RESULTS: Quantitative results of AIV and IBV co-infection showed that interferences between the two viruses yielded decreased viral growth. However, in the case of super-infection, the second virus, either AIV or IBV, induced a decrease in the growth of the first inoculated virus. CONCLUSION: It appears that either AIV or IBV has a negative impact on the other virus growth when they are inoculated simultaneously or sequentially. The ELISA results showed that higher level of secreted IL-1beta varies, depending on the viral interference conditions between both viruses, during mixed infections.
has issue date	2018-06-22(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.06.011
bibo:pmid	29940196
has license	no-cc
sha1sum (hex)	4e8c6fe77d84e9a04b3a275e508c4b281b3c05f0
schema:url	https://doi.org/10.1016/j.jviromet.2018.06.011
resource representing a document's title	Viral interference between low pathogenic avian influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo
has PubMed Central identifier	PMC7119724
has PubMed identifier	29940196
schema:publication	J Virol Methods
resource representing a document's body	covid:4e8c6fe77d84e9a04b3a275e508c4b281b3c05f0#body_text
is schema:about of	named entity 'interference' named entity 'IL-1' named entity 'determined' named entity 'infectious bronchitis' named entity 'interference' covid:arg/4e8c6fe77d84e9a04b3a275e508c4b281b3c05f0 named entity 'gene' named entity 'ECE' named entity 'Background' named entity 'IBV' named entity 'non-segmented' named entity 'infection' named entity 'embryo' named entity 'AIV' named entity '2012' named entity 'lung' named entity 'cell cultures' named entity 'AIV' named entity 'Viral' named entity 'ovo' named entity 'mixed infections' named entity 'virus' named entity 'chicken eggs' named entity 'embryo' named entity 'virus' named entity 'cell cultures' named entity 'IBV' named entity 'avian infectious bronchitis' named entity 'Tunisia' named entity 'genome' named entity 'supernatants' named entity 'H9N2' named entity 'IL-1' named entity 'titer' named entity 'gene' named entity 'MDCK' named entity 'allantoic' named entity 'viral replication' named entity 'Tunisia' named entity 'secretion' named entity 'trypsin' named entity 'tissue culture' named entity 'titer' named entity 'mixed infection' named entity 'viral infection' named entity 'cell cultures' named entity 'Coronaviruses' named entity 'multiple comparisons' named entity 'viral infection' named entity 'IBV' named entity 'organism' named entity 'ATCC' named entity 'rRT-PCR' named entity 'copy numbers' named entity 'AIV' named entity 'AIV' named entity 'infection' named entity 'Newcastle disease' named entity '0.01' named entity 'Vero' named entity 'host cells' named entity 'MDCK' named entity 'Invitrogen' named entity 'cultured cells' named entity 'AIV' named entity 'IBV' named entity 'virus replication' named entity 'allantoic' named entity 'infection' named entity 'hemorrhagic' named entity 'RNA viruses' named entity 'experimental groups' named entity 'infection' named entity 'AIV' named entity 'annealing' named entity 'AIV' named entity 'plasmid preparation' named entity '0.01' named entity 'titrated'

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