About: Engineering large viral DNA genomes using the CRISPR‐Cas9 system

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About: Engineering large viral DNA genomes using the CRISPR‐Cas9 system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Engineering large viral DNA genomes using the CRISPR‐Cas9 system
Creator	Arase, Hisashi Suenaga, Tadahiro Hirayasu, Kouyuki Kohyama, Masako
Source	Medline; PMC
abstract	Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus‐infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected recombination is quite low. Alternatively, large viral genomes have been edited using a bacterial artificial chromosome (BAC) plasmid system. However, cloning of large viral genomes into BAC plasmids is both laborious and time‐consuming. In addition, because it is possible for insertion into the viral genome of drug selection markers or parts of BAC plasmids to affect viral function, artificial genes sometimes need to be removed from edited viruses. Herpes simplex virus (HSV), a common DNA virus with a genome length of 152 kbp, causes labialis, genital herpes and encephalitis. Mutant HSV is a candidate for oncotherapy, in which HSV is used to kill tumor cells. In this study, the clustered regularly interspaced short palindromic repeat‐Cas9 system was used to very efficiently engineer HSV without inserting artificial genes into viral genomes. Not only gene‐ablated HSV but also gene knock‐in HSV were generated using this method. Furthermore, selection with phenotypes of edited genes promotes the isolation efficiencies of expectedly mutated viral clones. Because our method can be applied to other DNA viruses such as Epstein–Barr virus, cytomegaloviruses, vaccinia virus and baculovirus, our system will be useful for studying various types of viruses, including clinical isolates.
has issue date	2014-09-02(xsd:dateTime)
bibo:doi	10.1111/1348-0421.12180
bibo:pmid	25040500
has license	no-cc
sha1sum (hex)	4eab72e2ad803a93190d2fdd95f4d0787ec11cb3
schema:url	https://doi.org/10.1111/1348-0421.12180
resource representing a document's title	Engineering large viral DNA genomes using the CRISPR‐Cas9 system
has PubMed Central identifier	PMC7168497
has PubMed identifier	25040500
schema:publication	Microbiol Immunol
resource representing a document's body	covid:4eab72e2ad803a93190d2fdd95f4d0787ec11cb3#body_text
is schema:about of	named entity 'viruses' named entity 'genome' named entity 'plasmids' named entity 'vaccinia virus' named entity 'Homologous recombination' named entity 'baculovirus' named entity 'palindromic' named entity 'genes' named entity 'system' named entity 'DNA' named entity 'ISOLATES' named entity 'ADDITION' named entity 'VIRUSES' named entity 'HSV' named entity 'VIRAL GENE' covid:arg/4eab72e2ad803a93190d2fdd95f4d0787ec11cb3 named entity 'phenotypes' named entity 'viruses' named entity 'isolates' named entity 'generated' named entity 'affect' named entity 'developed' named entity 'Manipulation' named entity 'recombination' named entity 'kill' named entity 'selection' named entity 'gene mutations' named entity 'viral genomes' named entity 'candidate' named entity 'clones' named entity 'insertion' named entity 'viruses' named entity 'essential' named entity 'HSV' named entity 'BAC' named entity 'vaccinia virus' named entity 'Epstein-Barr virus' named entity 'HSV' named entity 'viruses' named entity 'HSV' named entity 'Homologous recombination' named entity 'clustered regularly interspaced short palindromic repeat' named entity 'cytomegaloviruses' named entity 'viral DNA' named entity 'kbp' named entity 'edited' named entity 'HSV-1' named entity 'gene editing' named entity 'plasmids' named entity 'cell surface marker' named entity 'sgRNA' named entity 'CRISPR-Cas9' named entity 'HSV-1' named entity 'genome' named entity 'HSV-1' named entity 'HSV-1' named entity 'CRISPR-Cas9' named entity 'sgRNA' named entity 'clones' named entity 'Escherichia coli' named entity 'plasmid' named entity 'flow cytometer' named entity 'mice' named entity 'sgRNA' named entity 'sgRNA' named entity 'viral genomes' named entity 'BD Biosciences' named entity 'transfected' named entity 'HSV-1' named entity 'amino acid residues' named entity 'HSV-1' named entity 'recombination' named entity 'IgG1' named entity 'CRISPR-Cas9' named entity 'HSV-1' named entity 'adenovirus' named entity 'CRISPR-Cas' named entity 'baculoviruses'

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