About: Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B

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About: Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B
Creator	Brummer, Mirko Eboigbodin, Kevin Elf, Sonja Filén, Sanna Hoser, Mark Ojalehto, Tuomas Pousi, Kirsi
Source	Medline; PMC
abstract	Rapid and accurate diagnosis of influenza viruses plays an important role in infection control, as well as in preventing the misuse of antibiotics. Isothermal nucleic acid amplification methods offer significant advantages over the polymerase chain reaction (PCR), since they are more rapid and do not require the sophisticated instruments needed for thermal cycling. We previously described a novel isothermal nucleic acid amplification method, ‘Strand Invasion Based Amplification’ (SIBA®), with high analytical sensitivity and specificity, for the detection of DNA. In this study, we describe the development of a variant of the SIBA method, namely, reverse transcription SIBA (RT-SIBA), for the rapid detection of viral RNA targets. The RT-SIBA method includes a reverse transcriptase enzyme that allows one-step reverse transcription of RNA to complementary DNA (cDNA) and simultaneous amplification and detection of the cDNA by SIBA under isothermal reaction conditions. The RT-SIBA method was found to be more sensitive than PCR for the detection of influenza A and B and could detect 100 copies of influenza RNA within 15 min. The development of RT-SIBA will enable rapid and accurate diagnosis of viral RNA targets within point-of-care or central laboratory settings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-016-7491-y) contains supplementary material, which is available to authorized users.
has issue date	2016-04-11(xsd:dateTime)
bibo:doi	10.1007/s00253-016-7491-y
bibo:pmid	27063012
has license	cc-by
sha1sum (hex)	4ef86c928be12807b78ebc327eb41f0380cf0b75
schema:url	https://doi.org/10.1007/s00253-016-7491-y
resource representing a document's title	Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B
has PubMed Central identifier	PMC4875950
has PubMed identifier	27063012
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:4ef86c928be12807b78ebc327eb41f0380cf0b75#body_text
is schema:about of	named entity 'STRAND INVASION' named entity 'targets' named entity 'infection control' named entity 'viruses' named entity 'reverse transcription' named entity 'amplification' named entity 'DETECTION' named entity 'ANALYTICAL' named entity 'INFLUENZA' named entity 'NOVEL' named entity 'DESCRIBED' named entity 'VARIANT' named entity 'DIAGNOSIS' named entity 'COPIES' named entity 'RNA' named entity 'PLAYS' named entity 'STUDY' named entity 'INFLUENZA VIRUSES' named entity 'SENSITIVE' named entity 'REVERSE TRANSCRIPTION' named entity 'PCR' named entity 'PREVENTING' named entity 'COMPLEMENTARY DNA' named entity 'DETECTION' named entity 'THERMAL' named entity 'CONDITIONS' named entity 'REVERSE TRANSCRIPTASE' named entity 'ANTIBIOTICS' named entity 'DNA' named entity 'METHODS' named entity 'CDNA' named entity 'ENZYME' named entity 'TARGETS' named entity 'AMPLIFICATION' named entity 'DO NOT' named entity 'OFFER' named entity 'INCLUDES' named entity 'INFECTION CONTROL' named entity 'STEP' named entity 'RAPID' named entity 'ALLOWS' named entity 'SIBA' named entity 'RAPID' named entity 'BASED' named entity 'METHOD' named entity 'DESCRIBE' named entity 'REACTION' named entity 'NUCLEIC ACID AMPLIFICATION METHOD' named entity 'INFLUENZA A' named entity 'METHOD' named entity 'SIMULTANEOUS' named entity 'MIN' named entity 'SIGNIFICANT' named entity 'DEVELOPMENT' named entity 'VIRAL RNA' named entity 'POINT-OF-CARE' named entity 'INSTRUMENTS' named entity 'INVASION' named entity 'DETECT' named entity 'NUCLEIC ACID AMPLIFICATION' named entity 'CENTRAL LABORATORY' named entity 'AMPLIFICATION' named entity 'SIBA' named entity 'IMPORTANT' named entity 'FOUND' named entity 'INFLUENZA A' named entity 'ENABLE' named entity 'HIGH' named entity 'ROLE' named entity 'POLYMERASE CHAIN REACTION' named entity 'PREVIOUSLY' named entity 'REVERSE TRANSCRIPTION' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'CYCLING' named entity 'BASED'

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