About: A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies

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About: A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies
Creator	Zambon, Maria Temperton, Nigel De Jong, Menno Hoschler, Katja Major, Diane Manvell, Ruth Nicolson, Carolyn Takeuchi, Yasuhiro Weiss, Robin Hien, Vo Ha, Do
Source	Medline; PMC
abstract	Background The World Health Organisation (WHO) recommended the development of simple, safe, sensitive and specific neutralization assays for avian influenza antibodies. We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2. Results Using our assay, sera from patients who had recovered from infection with influenza H5N1, and sera from animals experimentally immunized or infected with H5 tested positive for the presence of neutralizing antibodies to H5N1. Pseudotype neutralizing antibody titers were compared with titers obtained by hemagglutinin inhibition (HI) assays and microneutralization (MN) assays using live virus, and showed a high degree of correlation, sensitivity and specificity. Conclusions The pseudotype neutralization assay is as sensitive as horse erythrocyte HI and MN for the detection of antibodies to H5N1. It is safer, and can be applied in a high‐throughput format for human and animal surveillance and for the evaluation of vaccines.
has issue date	2007-07-26(xsd:dateTime)
bibo:doi	10.1111/j.1750-2659.2007.00016.x
bibo:pmid	19453415
has license	no-cc
sha1sum (hex)	4f1b29ad62f545bbbce05dcdf1d7a2ce0b72e1d4
schema:url	https://doi.org/10.1111/j.1750-2659.2007.00016.x
resource representing a document's title	A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies
has PubMed Central identifier	PMC4941878
has PubMed identifier	19453415
schema:publication	Influenza Other Respir Viruses
resource representing a document's body	covid:4f1b29ad62f545bbbce05dcdf1d7a2ce0b72e1d4#body_text
is schema:about of	named entity 'NEUTRALIZATION' named entity 'ANTIBODIES' named entity 'SAFE' named entity 'ASSAY' named entity 'H5N1' named entity 'RETROVIRAL' named entity 'RETROVIRAL' named entity 'HEMAGGLUTININ' named entity 'AVIAN INFLUENZA' named entity 'VIRUSES' named entity 'CARRIED' named entity 'SPECIFIC' named entity 'NEUTRALIZING ANTIBODIES' named entity 'SENSITIVE' named entity 'INFLUENZA' named entity 'WORLD HEALTH' named entity 'SURROGATE' named entity 'BACKGROUND' named entity 'HAVE' named entity 'BEARING' named entity 'SIMPLE' named entity 'DEVELOPMENT' named entity 'SENSITIVE' named entity 'ORGANISATION' named entity 'USED' named entity 'RECOMMENDED' named entity 'INFLUENZA' named entity 'BIOSAFETY LEVEL 2' covid:arg/4f1b29ad62f545bbbce05dcdf1d7a2ce0b72e1d4 named entity 'assays' named entity 'surrogate' named entity 'retroviral' named entity 'Biosafety Level 2' named entity 'antibodies' named entity 'H5N1' named entity 'assay' named entity 'MLV' named entity 'GFP' named entity 'viruses' named entity 'canine' named entity 'virus' named entity 'hemagglutinin inhibition' named entity '293T cells' named entity 'human sera' named entity 'hemagglutinin inhibition' named entity 'hemagglutinin' named entity 'expression vector' named entity 'erythrocytes' named entity 'SARS-CoV' named entity 'Health Protection Agency' named entity 'MLV' named entity 'turkey' named entity 'endemic' named entity 'virus' named entity 'sialic acid' named entity 'virus' named entity 'vaccine' named entity 'HIV' named entity 'virus' named entity 'Sheep' named entity 'HIV' named entity 'systemic infection' named entity 'MLV' named entity 'envelope protein' named entity 'retroviral' named entity 'antibodies' named entity 'porcine' named entity 'influenza virus' named entity 'virus' named entity 'correlation' named entity 'neutralizing antibodies'

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