About: Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP

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About: Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP
Creator	Wang, Yi Chen, Yu Sun, Ying Guo, Deyin Jin, Xu Wu, Andong Zhang, Zhou Yu, Xiao Zeng, Cong Tao, Jiali Tian, Jie
Source	Elsevier; Medline; PMC
abstract	Abstract Most eukaryotic viruses that replicate in the cytoplasm, including coronaviruses, have evolved strategies to cap their RNAs. In our previous work, the nonstructural protein (nsp) 14 of severe acute respiratory syndrome coronavirus (SARS-CoV) was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). In this study, we found that GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by SARS-CoV nsp14. In contrast, the nsp14 could not modify ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA. Critical residues of nsp14 essential for the methyltransferase activity on GTP were identified, which include F73, R84, W86, R310, D331, G333, P335, Y368, C414, and C416. We further showed that the methyltransferase activity of GTP was universal for nsp14 of other coronaviruses. Moreover, the accumulation of m7GTP or presence of protein nsp14 could interfere with protein translation of cellular mRNAs. Altogether, the results revealed a new enzymatic activity of coronavirus nsp14.
has issue date	2013-09-30(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2013.05.001
bibo:pmid	23702198
has license	els-covid
sha1sum (hex)	4f88e7da806c41f481d1243ec6d3581d1696c360
schema:url	https://doi.org/10.1016/j.virusres.2013.05.001
resource representing a document's title	Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP
has PubMed Central identifier	PMC7114466
has PubMed identifier	23702198
schema:publication	Virus Research
resource representing a document's body	covid:4f88e7da806c41f481d1243ec6d3581d1696c360#body_text
is schema:about of	named entity 'METHYLTRANSFERASE ACTIVITY' named entity 'SARS-CoV' named entity 'UTP' named entity 'cellular' named entity 'severe acute respiratory syndrome coronavirus' named entity 'dCTP' named entity 'R310' named entity 'methyltransferase' named entity 'NSP14' named entity 'NUCLEOTIDE' named entity 'DUTP' named entity 'ANALOG' named entity 'PROTEIN ' named entity 'DGTP' named entity 'CORONAVIRUS' named entity 'CONTRAST' named entity 'ACCUMULATION' named entity 'COULD BE' named entity 'EVOLVED' named entity 'METHYLTRANSFERASE ACTIVITY' named entity 'RNAS' named entity 'WORK' named entity 'THEIR' named entity 'METHYLTRANSFERASE' named entity 'INTERFERE' named entity 'ATP' named entity 'CHARACTERIZATION' named entity 'SARS-COV' named entity 'MODIFY' named entity 'CORONAVIRUS' named entity 'GTP' named entity 'M7GPPPA' named entity 'W86' named entity 'MTASE' named entity 'ENZYMATIC ACTIVITY' named entity 'GUANINE' named entity 'ESSENTIAL' named entity 'EUKARYOTIC' named entity 'CORONAVIRUSES' named entity 'DATP' named entity 'CRITICAL' named entity 'PRESENCE OF' named entity 'UNIVERSAL' named entity 'INCLUDING' named entity 'CAP' named entity 'REVEALED' named entity 'OUR' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'RESULTS' named entity 'IDENTIFIED' named entity 'MRNAS' named entity 'F73' named entity 'CELLULAR' named entity 'PREVIOUS' named entity 'HAVE' named entity 'INCLUDE' named entity 'REPLICATE' named entity 'CTP' named entity 'STUDY' named entity 'R84' named entity 'CYTOPLASM' named entity 'METHYLATED' named entity 'NEW' named entity 'ANALOGS' named entity 'GTP' named entity 'UTP' named entity 'PROTEIN TRANSLATION' named entity 'DCTP' named entity 'STRATEGIES' named entity 'FOUND' named entity 'VIRUSES'

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