About: Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP)

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About: Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP)
Creator	Faulstich, Konrad Polavarapu, Rathnagiri Surabattula, Rambabu Mallepaddi, Chand Pradeep Vejandla, Manju
source	Elsevier; Medline; PMC
abstract	Abstract We attempted to improve the loop-mediated isothermal amplification (LAMP) method for malaria diagnosis by using a simple DNA extraction procedure, and a portable device performing both the amplification and detection of LAMP in one platform. Additionally, the device served as a heating block for the DNA preparation. We refer this method as LAMP-Tube scanner, and evaluated using 209 microscopically positive malaria samples and compared them to RDTs and LAMP-Thermocycler. Two most common human infecting Plasmodium species were detected. The LAMP-Tube scanner method is found to be simple and allowed real-time detection of DNA amplification. The time to amplification varied but was closely less than 60min. Sensitivity and specificity of LAMP-Tube scanner in detecting Plasmodium falciparum were 95% and 93.3%, compared to microscopy and 98.3% and 100% respectively, compared to standard LAMP-Thermocycler. In addition, it showed a detection limit of 10 and 40 copies of the parasitemia for Plasmodium vivax and P. falciparum. Accordingly, in comparison to the results obtained by microscopy, the LAMP-Tube scanner had a less divergence in sensitivity and specificity, and yielded results similar to those of LAMP-Thermocycler. This method has the great potential as a field usable molecular tool for the diagnosis of malaria and is an alternative to conventional PCR-based diagnostic methods for field use.
has issue date	2013-07-31(xsd:dateTime)
bibo:doi	10.1016/j.exppara.2013.03.031
bibo:pmid	23562879
has license	els-covid
sha1sum (hex)	4fa58c18ee726c80f2677cef1665716babb728a8
schema:url	https://doi.org/10.1016/j.exppara.2013.03.031
resource representing a document's title	Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP)
has PubMed Central identifier	PMC7094605
has PubMed identifier	23562879
schema:publication	Experimental Parasitology
resource representing a document's body	covid:4fa58c18ee726c80f2677cef1665716babb728a8#body_text
is schema:about of	named entity 'Thermocycler' named entity 'global climate change' named entity 'Plasmodium' named entity 'parasites' named entity 'Thermocycler' named entity 'DNA extraction' named entity 'antigen-based' named entity 'loop-mediated isothermal amplification' named entity 'malaria infection' named entity 'R-square' named entity 'QIAGEN' named entity 'microscopist' named entity 'nested PCR' named entity 'fluorescence detector' named entity 'lactate dehydrogenase' named entity 'malaria' named entity 'P. vivax' named entity 'P. falciparum' named entity 'microscopy' named entity 'TE buffer' named entity 'point-of-care' named entity 'false-negative' named entity 'Andhra Pradesh' named entity 'Germany' named entity 'syringe filter' named entity 'rRNA' named entity 'Li-Ion' named entity 'parasite' named entity 'Thermocycler' named entity 'influenza virus' named entity 'sensitivities and specificities' named entity 'dipstick' named entity 'filtrate' named entity 'parasitemia' named entity 'lateral flow' named entity 'genus' named entity 'negative film' named entity 'point-of-care' named entity 'parasites' named entity 'false positives' named entity 'Thermocycler' named entity 'parasitemia' named entity 'HRP-2' named entity 'fluorescence' named entity '18S rRNA' named entity 'blood films' named entity 'RTDs' named entity 'misdiagnosis' named entity 'Thermocycler' named entity 'sensitivity and specificity' named entity 'linear correlation' named entity 'malaria' named entity 'parasitemia' named entity 'Thermocycler' named entity 'False positive results' named entity 'fluorescent' named entity 'DNA extraction' named entity 'P. falciparum' named entity 'P. vivax' named entity 'Lake Constance' named entity 'Thermocycler' named entity 'P. vivax' named entity 'fluorescence' named entity 'serological' named entity 'P. vivax' named entity 'P. falciparum' named entity 'Germany' named entity 'human DNA' named entity 'malaria' named entity 'diagnostic test' named entity 'Thermocycler' named entity 'Taq polymerase' named entity 'parasitemia' named entity 'Malaria' named entity 'Qiagen' named entity 'plasmid DNA' named entity 'DNA extraction'

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