About: Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches

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About: Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches
Creator	Wang, Yuefei Wang, Zhihua Dong, Shishang Fu, Xu Jiang, Zhenzuo Li, Lixin Li, Zhucui Shui, Wenqing
Source	PMC
abstract	The nucleoprotein (NP) of Ebola virus (EBOV) and Marburg virus (MARV) is an essential component of the viral ribonucleoprotein complex and significantly impacts replication and transcription of the viral RNA genome. Although NP is regarded as a promising antiviral druggable target, no chemical ligands have been reported to interact with EBOV NP or MARV NP. We identified two compounds from a traditional Chinese medicine Gancao (licorice root) that can bind both NPs by combining affinity mass spectrometry and metabolomics approaches. These two ligands, 18β-glycyrrhetinic acid and licochalcone A, were verified by defined compound mixture screens and further characterized with individual ligand binding assays. Accompanying biophysical analyses demonstrate that binding of 18β-glycyrrhetinic acid to EBOV NP significantly reduces protein thermal stability, induces formation of large NP oligomers, and disrupts the critical association of viral ssRNA with NP complexes whereas the compound showed no such activity on MARV NP. Our study has revealed the substantial potential of new analytical techniques in ligand discovery from natural herb resources. In addition, identification of a chemical ligand that influences the oligomeric state and RNA-binding function of EBOV NP sheds new light on antiviral drug development.
has issue date	2016-07-12(xsd:dateTime)
bibo:doi	10.1038/srep29680
bibo:pmid	27403722
has license	cc-by
sha1sum (hex)	4fcb098e746e236ebc700fc79262c3263550b3d9
schema:url	https://doi.org/10.1038/srep29680
resource representing a document's title	Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches
has PubMed Central identifier	PMC4940736
has PubMed identifier	27403722
schema:publication	Sci Rep
resource representing a document's body	covid:4fcb098e746e236ebc700fc79262c3263550b3d9#body_text
is schema:about of	named entity 'EBOLA VIRUS' named entity 'AFFINITY' named entity 'light' named entity 'induces' named entity 'replication' named entity 'antiviral' named entity 'formation' named entity 'Affinity' named entity 'CHEMICAL' named entity 'LARGE' named entity 'IDENTIFIED' named entity 'MIXTURE' named entity 'STATE' named entity 'OLIGOMERS' named entity 'VERIFIED' named entity 'DISCOVERY' named entity 'LIGAND BINDING' named entity 'BINDING ASSAYS' named entity 'DISRUPTS' named entity 'REPLICATION' named entity 'MASS SPECTROMETRY' named entity 'ACTIVITY' named entity 'STUDY' named entity 'ROOT' named entity 'RIBONUCLEOPROTEIN COMPLEX' named entity 'OUR' named entity 'SSRNA' named entity 'BIOPHYSICAL' named entity 'VIRAL RNA' named entity 'ASSOCIATION' named entity 'COMBINING' named entity 'CRITICAL' named entity 'REVEALED' named entity 'EBOV NP' named entity 'LIGAND' named entity 'RESOURCES' named entity 'METABOLOMICS' named entity 'TRADITIONAL CHINESE MEDICINE' named entity 'LIGHT' named entity 'ADDITION' named entity 'TRANSCRIPTION' named entity 'NEW' named entity 'FORMATION' named entity 'CHEMICAL' named entity 'METABOLOMICS' named entity 'MARBURG VIRUS ' named entity 'MASS SPECTROMETRY' named entity 'ANTIVIRAL DRUG' named entity 'REPORTED' named entity 'TARGET' named entity 'LICOCHALCONE A' named entity 'CHARACTERIZED' named entity 'COMPOUND' named entity 'DRUG DEVELOPMENT' named entity 'THESE' named entity 'ACCOMPANYING' named entity 'APPROACHES' named entity 'INDIVIDUAL' named entity 'BINDING FUNCTION' named entity 'NOVEL' named entity 'COMBINING' named entity 'IDENTIFIED BY' named entity 'NUCLEOPROTEINS' named entity 'LIGANDS' named entity 'BINDING' named entity 'GLYCYRRHETINIC ACID' named entity 'DEFINED' named entity 'BIND' named entity 'PROTEIN ' named entity 'ANTIVIRAL' named entity 'VIRAL' named entity 'COMPLEXES' named entity 'HAVE' named entity 'LIGANDS'

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