About: Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells

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About: Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
Creator	Bai, Bingke Mao, Panyong Shi, Zhengli Zhou, Peng Hu, Hui Hu, Qinxue Huang, Yi Lu, Baojing Meng, Jin Wang, Hanzhong
Source	Medline; PMC
abstract	The pathogenesis of SARS coronavirus (CoV) remains poorly understood. In the current study, two recombinant baculovirus were generated to express the spike (S) protein of SARS-like coronavirus (SL-CoV) isolated from bats (vAcBS) and the envelope (E) and membrane (M) proteins of SARS-CoV, respectively. Co-infection of insect cells with these two recombinant baculoviruses led to self-assembly of virus-like particles (BVLPs) as demonstrated by electron microscopy. Incorporation of S protein of vAcBS (BS) into VLPs was confirmed by western blot and immunogold labeling. Such BVLPs up-regulated the level of CD40, CD80, CD86, CD83, and enhanced the secretion of IL-6, IL-10 and TNF-α in immature dendritic cells (DCs). Immune responses were compared in immature DCs inoculated with BVLPs or with VLPs formed by S, E and M proteins of human SARS-CoV. BVLPs showed a stronger ability to stimulate DCs in terms of cytokine induction as evidenced by 2 to 6 fold higher production of IL-6 and TNF-α. Further study indicated that IFN-γ+ and IL-4+ populations in CD4+ T cells increased upon co-cultivation with DCs pre-exposed with BVLPs or SARS-CoV VLPs. The observed difference in DC-stimulating activity between BVLPs and SARS CoV VLPs was very likely due to the S protein. In agreement, SL-CoV S DNA vaccine evoked a more vigorous antibody response and a stronger T cell response than SARS-CoV S DNA in mice. Our data have demonstrated for the first time that SL-CoV VLPs formed by membrane proteins of different origins, one from SL-CoV isolated from bats (BS) and the other two from human SARS-CoV (E and M), activated immature DCs and enhanced the expression of co-stimulatory molecules and the secretion of cytokines. Finding in this study may provide important information for vaccine development as well as for understanding the pathogenesis of SARS-like CoV.
has issue date	2008-07-16(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0002685
bibo:pmid	18628832
has license	cc-by
sha1sum (hex)	505d4036449010b44702841ae2564d6a55a3fbcd
schema:url	https://doi.org/10.1371/journal.pone.0002685
resource representing a document's title	Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
has PubMed Central identifier	PMC2441860
has PubMed identifier	18628832
schema:publication	PLoS One
resource representing a document's body	covid:505d4036449010b44702841ae2564d6a55a3fbcd#body_text
is schema:about of	named entity 'activated' named entity 'mice' named entity 'Co-infection' named entity 'secretion' named entity 'cells' named entity 'Further' named entity 'DIFFERENT' named entity 'FORMED' named entity 'VIRUS-LIKE PARTICLES' named entity 'RECOMBINANT' named entity 'DCS' named entity 'PROTEINS' named entity 'HIGHER' named entity 'UNDERSTOOD' named entity 'OUR' named entity 'FINDING' named entity 'IL-4' named entity 'CURRENT' named entity 'BACULOVIRUS' named entity 'T CELLS' named entity 'CD40' named entity 'STIMULATING' named entity 'IMMUNE RESPONSES' named entity 'DENDRITIC CELLS' named entity 'EXPOSED' named entity 'PROTEIN ' named entity 'TO EXPRESS' named entity 'DNA VACCINE' named entity 'IMPORTANT' named entity 'PROVIDE' named entity 'VERY LIKELY' named entity 'UNDERSTANDING' named entity 'SPIKE' named entity 'CELLS' named entity 'CONFIRMED BY' named entity 'BATS' named entity 'ORIGINS' named entity 'INSECT' named entity 'THESE' named entity 'PRODUCTION' named entity 'CD4' named entity 'ENHANCED' named entity 'IL-6' named entity 'ELECTRON MICROSCOPY' named entity 'FOLD' named entity 'INFORMATION' named entity 'MEMBRANE PROTEINS' named entity 'DNA' named entity 'SARS' named entity 'ENVELOPE' named entity 'CD83' named entity 'CD80' named entity 'SARS-LIKE CORONAVIRUS' named entity 'INDICATED' named entity 'SARS-COV' named entity 'VACCINE DEVELOPMENT' named entity 'UP-REGULATED' named entity 'DIFFERENCE' named entity 'BACULOVIRUSES' named entity 'DIFFERENT' named entity 'DEMONSTRATED' named entity 'INCREASED' named entity 'LEVEL' named entity 'CYTOKINE ' named entity 'CULTIVATION' named entity 'IMMATURE' named entity 'MOLECULES' named entity 'STIMULATE' named entity 'LIKE' named entity 'OBSERVED' named entity 'STIMULATING' named entity 'SELF-ASSEMBLY'

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