About: The receptor for Bacillus thuringiensis CrylA(c) delta‐endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N

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About: The receptor for Bacillus thuringiensis CrylA(c) delta‐endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The receptor for Bacillus thuringiensis CrylA(c) delta‐endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N
Creator	Crickmore, Neii Eiiar, David Knight, Peter
Source	PMC
abstract	A 120 kDa glycoprotein in the larval midgut membrane of the Iepidopteran Manduca sexta, previously identified as a putative receptor for Bacillus thuringiensis CrylA(c) δ‐endotoxin, has been purified by a combination of protoxin affinity Chromatography and anion exchange chromatography. In immunoblotting experiments, the purified glycoprotein has the characteristics predicted of the receptor: it binds CrylA(c) toxin In the presence of GlcNAc but not GalNAc; it binds the lectin SBA; but it does not bind CrylB toxin. N‐terminal and internal amino acid sequences obtained from the protein show a high degree of similarity with the enzyme aminopeptidase N (EC 3.4.11.2). When assayed for aminopeptidase activity, purified receptor preparations were enriched 5.3‐fold compared to M. sexta brush border membrane vesicles. We propose that the receptor for CrylA(c) toxin in the brush border membrane of the lepidopteran M. sexta is the metalloprotease aminopeptidase N.
has issue date	2006-10-27(xsd:dateTime)
bibo:doi	10.1111/j.1365-2958.1994.tb00324.x
bibo:pmid	7908713
has license	no-cc
sha1sum (hex)	506f026cb8f4cfa9172251a0a12ea663a5cc8777
schema:url	https://doi.org/10.1111/j.1365-2958.1994.tb00324.x
resource representing a document's title	The receptor for Bacillus thuringiensis CrylA(c) delta‐endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N
has PubMed Central identifier	PMC7168503
has PubMed identifier	7908713
schema:publication	Mol Microbiol
resource representing a document's body	covid:506f026cb8f4cfa9172251a0a12ea663a5cc8777#body_text
is schema:about of	named entity 'similarity' named entity 'combination' named entity 'aminopeptidase N' named entity 'EXPERIMENTS' named entity 'AMINO ACID SEQUENCES' named entity 'SBA' named entity 'IDENTIFIED' named entity '3.4.11.2' named entity 'METALLOPROTEASE' named entity 'glycoprotein' named entity 'toxin' named entity 'bind' named entity 'Manduca sexta' named entity 'toxin' named entity 'immunoblotting' named entity 'M. sexta' named entity 'midgut' named entity 'endotoxin' named entity 'brush border' named entity 'midgut' named entity 'GalNAc' named entity 'kDa' named entity 'lectin' named entity 'receptor' named entity 'M. sexta' named entity 'enzymatic activity' named entity 'aminopeptidase' named entity 'protoxin' named entity 'trifluoroacetic acid' named entity 'aminopeptidase' named entity 'M. sexta' named entity 'toxin' named entity 'bovine' named entity 'hydrolysed' named entity 'w/v' named entity 'kDa' named entity 'SDS-PAGE' named entity 'M. sexta' named entity 'toxin' named entity 'aminopeptidase' named entity 'oligonucleotides' named entity 'protoxin' named entity 'aminopeptidase' named entity 'Manduca sexta' named entity 'PCR' named entity 'anti-toxin' named entity 'PMSF' named entity 'eluted' named entity 'aminopeptidase' named entity 'insect' named entity 'toxin' named entity 'DTT' named entity 'lepidopteran' named entity 'midguts' named entity 'Amino acid sequences' named entity 'M. sexta' named entity 'aminopeptidase' named entity 'diethanolamine' named entity 'dialysed' named entity 'kDa' named entity 'protoxin' named entity 'trypsin' named entity 'hydrophobic residues' named entity 'amino acid sequence' named entity 'protoxin' named entity 'protein' named entity 'kDa' named entity 'toxin' named entity 'toxin' named entity 'aminopeptidase' named entity 'detergent' named entity 'silver staining' named entity 'Affinity chromatography' named entity 'glycoprotein' named entity 'glass fibre' named entity 'M. sexta' named entity 'M. sexta' named entity 'receptor binding' named entity 'TCA'

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