About: Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip

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About: Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip
Creator	Roh, Changhyun Kee, Sung
Source	Medline; PMC
abstract	BACKGROUND: Globally, severe acute respiratory syndrome coronavirus (SARS‐CoV) is a newly emerging virus that causes SARS with high mortality rate in infected people. The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)‐associated coronavirus (SARS‐CoV) is an important antigen for the early diagnosis of SARS and the detection of diseases. Here, a new quantum dots (QDs)‐conjugated RNA aptamer with high sensitivity and rapidity is proposed for the detection of SARS‐CoV N protein using an on chip system. RESULTS: A QDs‐conjugated RNA aptamer can specifically hybridize on the immobilized SARS‐CoV N protein on the surface of a glass chip. Detection is based on the optical signal variation of a QDs‐supported RNA aptamer interacting on an immobilized protein chip. Using an optical QDs‐based RNA aptamer chip, SARS N protein was detected at concentrations as low as 0.1 pg mL(−1). CONCLUSIONS: It was demonstrated that the QDs‐conjugated RNA aptamer could interact on a designed chip specifically and sensitively. This device could form a QDs‐conjugated biosensor prototype chip for SARS‐CoV N protein diagnosis. The proposed visual SARS‐CoV N protein detection technique may avoid the limitations of other reported methods because of its high sensitivity, good specificity, ease of use, and the ability to perform one‐spot monitoring. Copyright © 2011 Society of Chemical Industry
has issue date	2011-10-03(xsd:dateTime)
bibo:doi	10.1002/jctb.2721
bibo:pmid	32336860
has license	no-cc
sha1sum (hex)	50c102f5e1937d929ee00f76596023433ad376c6
schema:url	https://doi.org/10.1002/jctb.2721
resource representing a document's title	Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip
has PubMed Central identifier	PMC7167159
has PubMed identifier	32336860
schema:publication	J Chem Technol Biotechnol
resource representing a document's body	covid:50c102f5e1937d929ee00f76596023433ad376c6#body_text
is schema:about of	named entity 'RNA APTAMER' named entity 'CHIP' named entity 'antigen' named entity 'nucleocapsid' named entity 'assay' named entity 'antibodies' named entity 'SARS-CoV' named entity 'Tris buffer' named entity 'RNA aptamer' named entity 'SARS-CoV' named entity 'denaturation' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'nucleocapsid' named entity 'RNA polymerase' named entity 'immunosorbent assay' named entity 'fluorescent dyes' named entity 'E. coli' named entity 'SalI' named entity 'RNA' named entity 'detection limit' named entity 'protein' named entity 'diagnostic medicine' named entity 'PCR' named entity 'Stratagene' named entity 'imidazole' named entity 'genome' named entity 'pmol' named entity 'RNA aptamer' named entity 'RNA' named entity 'SARS' named entity 'nucleocapsid' named entity 'SARS' named entity 'nucleocapsid' named entity 'La Jolla' named entity 'RNA' named entity 'nucleocapsid' named entity 'gene' named entity 'reagent' named entity 'transcription' named entity 'secondary structure' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'protein' named entity 'protein' named entity 'nucleocapsid' named entity 'nanotechnology' named entity 'solubility' named entity 'antibodies' named entity 'early diagnosis' named entity 'nucleocapsid' named entity 'functional group' named entity 'amide' named entity 'T7 promoter' named entity 'agarose gel' named entity 'biosensors' named entity 'Aptamers' named entity 'sonication' named entity 'false-positive' named entity 'Promega' named entity 'aptamer' named entity 'pH 8' named entity 'protein' named entity 'nucleocapsid' named entity 'recombinant' named entity 'PMSF' named entity 'primer' named entity 'aptamer' named entity 'Sonicator' named entity 'Quantum dots' named entity 'amide' named entity 'nuclear localization signal' named entity 'RNA aptamer' named entity 'SARS-CoV' named entity 'SalI' named entity 'SARS-CoV' named entity 'SARS-CoV' named entity 'diagnostic tool' named entity 'SARS' named entity 'nucleocapsid'

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