About: Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs

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About: Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs
Creator	Liu, Xiangtao Li, Dong Bai, Xingwen Bao, Huifang Cao, Yimei Chen, Yingli Guo, Jianhong Liu, Zaixin Lu, Zengjun Sun, Jiachuan Sun, Pu
Source	Elsevier; Medline; PMC
abstract	The assembly of foot-and-mouth disease virus (FMDV) requires the cleavage of the P12A polyprotein into individual structural proteins by protease 3C. In this study, we constructed a recombinant baculovirus that simultaneously expressed the genes for the P12A and 3C proteins of Asia I FMDV from individual promoters. The capsid proteins expressed in High Five™ insect cells were processed by viral 3C protease, as shown by Western blotting, and were antigenic, as revealed by their reactivity in an indirect sandwich-ELISA, and by immunofluorescent assay. The empty capsid-like particles were similar to authentic 75S empty capsids from FMDV in terms of their shape, size and sedimentation velocity, as demonstrated by sucrose gradient centrifugation. Both empty capsid-like particles and some small-sized particles (about 10 nm in diameter) were also observed using immunoelectron microscopy. Furthermore, the empty capsid-like particles or intermediates induced high levels of FMDV-specific antibodies in guinea pigs following immunization, and neutralizing antibodies were induced in the second week after vaccination. These recombinant, non-infectious, FMDV empty capsids are potentially useful for the development of new diagnostic techniques and vaccines.
has issue date	2009-05-28(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2008.12.007
bibo:pmid	19167843
has license	no-cc
sha1sum (hex)	51f56783febe69530ae6fb6bd2725cbf0ac490a4
schema:url	https://doi.org/10.1016/j.vetmic.2008.12.007
resource representing a document's title	Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs
has PubMed Central identifier	PMC7117335
has PubMed identifier	19167843
schema:publication	Vet Microbiol
resource representing a document's body	covid:51f56783febe69530ae6fb6bd2725cbf0ac490a4#body_text
is schema:about of	named entity 'THEIR' named entity 'CELLS' named entity 'ASIA' named entity 'CAPSID' named entity 'FOOT-AND-MOUTH DISEASE VIRUS' named entity 'INSECT' named entity 'SYNTHESIS' named entity 'PARTICLES' named entity 'IMMUNOGENICITY' named entity 'LIKE' named entity 'GUINEA PIGS' named entity 'capsid' named entity 'virions' named entity 'Norwalk-like viruses' named entity 'positive and negative controls' named entity 'capsids' named entity 'capsid' named entity 'plasmids' named entity 'viruses' named entity 'self-assembled' named entity 'guinea pig' named entity 'epitopes' named entity 'guinea pigs' named entity 'virus' named entity 'homologous' named entity 'primers' named entity 'capsids' named entity 'capsid' named entity 'centrifugation' named entity 'vaccine' named entity 'FMDV' named entity '12,000' named entity 'inoculated' named entity 'capsid' named entity 'Invitrogen' named entity 'cloning' named entity 'capsid' named entity 'protein expression' named entity 'serotype' named entity 'virions' named entity 'recombinant protein' named entity 'IgG' named entity 'guinea pigs' named entity 'infectivity' named entity 'capsid' named entity 'virus' named entity 'insect' named entity 'antigen' named entity 'FMDV' named entity 'centrifugation' named entity 'capsid' named entity '1:32' named entity 'subunit vaccine' named entity 'baculovirus' named entity 'guinea pig' named entity 'neutralizing antibodies' named entity 'cell lysates' named entity 'infection' named entity 'subunit vaccine' named entity 'immunogenicity' named entity 'Invitrogen' named entity 'non-infectious' named entity 'IgG antibody' named entity 'serum' named entity 'FMDV' named entity 'transfection' named entity 'VP1' named entity 'protein' named entity 'sucrose' named entity 'vaccination' named entity 'FMDV'

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