About: Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients
Creator	Xu, Yang Di, Biao Zhang, Ying Cao, Lan Huang, Bing Li, Kuibiao Wu, Yong Xu, Zhi Zhang, Zhoubin Zhu, Pingting
Source	Medline; PMC
abstract	BACKGROUND: Respiratory viruses, such as influenza viruses, initially infect the upper airways but can manifest as severe lower respiratory tract infections in high‐risk patients with significant morbidity and mortality. For syndromic diagnosis, several multiplex nucleic acid amplification tests have been developed for clinics, of which SureX 13 Respiratory Pathogen Multiplex Kit (ResP) can simultaneously detect 13 pathogens directly from airway secretion specimens. The organisms identified are influenza virus A, influenza virus A pdmH1N1 (2009), influenza virus A H3N2, influenza virus B, adenovirus, boca virus, rhinovirus, parainfluenza virus, coronavirus, respiratory syncytial virus, human metapneumovirus, Mycoplasma pneumoniae, and Chlamydia. METHODS: This study provides performance evaluation data of this assay by comparing with pathogen‐specific PCRs from oropharyngeal swab samples. RESULTS: Ten pathogens were detected in this assay, of which rhinovirus, adenovirus, and influenza virus A pdmH1N1 (2009) were the most common. The overall agreement between the ResP and the comparator tests was 93.8%. The ResP demonstrated 86.5% agreement for positive results and 97.8% agreement for negative results. CONCLUSION: The ResP assay demonstrated a highly concordant performance comparing with pathogen‐specific PCRs for detection of respiratory pathogens in oropharyngeal swabs from outpatients and could aid in the diagnosis of respiratory infections in a variety of clinical scenarios.
has issue date	2019-10-19(xsd:dateTime)
bibo:doi	10.1002/jcla.23032
bibo:pmid	31628684
has license	cc-by
sha1sum (hex)	5349f364152c9a5e133f282cf5a1bd2449f8930b
schema:url	https://doi.org/10.1002/jcla.23032
resource representing a document's title	Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients
has PubMed Central identifier	PMC6977335
has PubMed identifier	31628684
schema:publication	J Clin Lab Anal
resource representing a document's body	covid:5349f364152c9a5e133f282cf5a1bd2449f8930b#body_text
is schema:about of	named entity 'MYCOPLASMA PNEUMONIAE' named entity 'amplification' named entity 'diagnosis' named entity 'influenza' named entity 'Mycoplasma pneumoniae' named entity 'SAMPLES' named entity 'RHINOVIRUS' named entity 'INFLUENZA VIRUS B' named entity 'LOWER RESPIRATORY TRACT INFECTIONS' named entity 'SEVERE' named entity 'SIMULTANEOUSLY' named entity 'SPECIMENS' named entity 'HAVE' named entity 'BUT' named entity 'MANIFEST' named entity 'AIRWAYS' named entity 'HIGH-RISK' named entity 'H3N2' named entity 'SIGNIFICANT' named entity 'DIRECTLY' named entity 'SECRETION' named entity 'VIRUS' named entity 'OROPHARYNGEAL SWAB' named entity 'ADENOVIRUS' named entity 'MORBIDITY AND MORTALITY' named entity 'INFLUENZA VIRUSES' named entity 'BACKGROUND' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'TESTS' named entity 'INFECT' named entity 'CHLAMYDIA' named entity 'PCR ASSAY' named entity 'RESPIRATORY VIRUSES' named entity 'CORONAVIRUS' named entity 'DETECTION' named entity 'OUTPATIENTS' named entity 'EVALUATION' named entity 'ORGANISMS' named entity 'CLINICS' named entity 'DIAGNOSIS' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'RESPIRATORY' named entity 'IDENTIFIED' named entity 'DETECT' named entity 'AIRWAY' named entity 'INFLUENZA VIRUS A' named entity 'PATHOGEN' named entity 'MULTIPLEX PCR' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'NUCLEIC ACID AMPLIFICATION' named entity 'PATIENTS' named entity 'INITIALLY' named entity 'PARAINFLUENZA VIRUS' named entity 'RESPIRATORY VIRUSES' named entity 'MULTIPLEX' named entity 'BOCA' named entity 'KIT' named entity 'SYNDROMIC' named entity 'UPPER' named entity 'PATHOGENS' covid:arg/5349f364152c9a5e133f282cf5a1bd2449f8930b named entity 'organisms' named entity 'acid' named entity 'identified' named entity 'Chlamydia' named entity 'initially' named entity 'viruses' named entity 'supernatant' named entity 'NAAT' named entity 'influenza virus' named entity 'PCR amplification' named entity 'influenza virus'

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