About: Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin

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About: Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin
Creator	Carrera, Mónica Mateos, Jesús Polak, Iwona Stryiński, Robert
Source	Medline; PMC
abstract	Ivermectin (IVM), an antiparasitic drug, has a positive effect against Anisakis simplex s.s. infection and has been used for the treatment and prevention of anisakiasis in humans. However, the molecular mechanism of action of IVM on A. simplex s.s. remains unknown. Herein, tandem mass tag (TMT) labeling and extensive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis were used to identify the effect of IVM on the proteome of A. simplex s.s. in vitro. During the study, 3433 proteins, of which 1247 had at least two protein unique peptides, were identified. Comparative proteomics analysis revealed that 59 proteins were differentially regulated (DRPs) in IVM-treated larvae, of which 14 proteins were upregulated and 38 were downregulated after 12 h of culture, but after 24 h, 12 proteins were upregulated and 22 were downregulated. The transcription level of five randomly selected DRPs was determined by real-time PCR as a supplement to the proteomic data. The functional enrichment analysis showed that most of the DRPs were involved in oxidoreductase activity, immunogenicity, protein degradation, and other biological processes. This study has, for the first time, provided comprehensive proteomics data on A. simplex s.s. response to IVM and might deliver new insight into the molecular mechanism by which IVM acts on invasive larvae of A. simplex s.s.
has issue date	2020-06-26(xsd:dateTime)
bibo:doi	10.3390/genes11060710
bibo:pmid	32604878
has license	cc-by
sha1sum (hex)	539222ef9a909418c9b02d78cb2331a2d865a2e9
schema:url	https://doi.org/10.3390/genes11060710
resource representing a document's title	Comparative Proteomics Analysis of Anisakis simplex s.s.—Evaluation of the Response of Invasive Larvae to Ivermectin
has PubMed Central identifier	PMC7349835
has PubMed identifier	32604878
schema:publication	Genes (Basel)
resource representing a document's body	covid:539222ef9a909418c9b02d78cb2331a2d865a2e9#body_text
is schema:about of	named entity 'humans' named entity 'downregulated' named entity 'data' named entity 'simplex' named entity 'mechanism' named entity 'mechanism' named entity 's.s.' named entity 'Analysis' named entity 'Evaluation' named entity 'IVM' named entity 'simplex' named entity 'simplex' named entity 'involved' named entity 'proteins' named entity 'upregulated' named entity 'labeling' named entity 'larvae' named entity 'IVM' named entity 'study' named entity 'Comparative' named entity 'anisakiasis' named entity 'study' named entity 'peptides' named entity 'immunogenicity' named entity 'LC-MS/MS' named entity 'oxidoreductase' named entity 'protein degradation' named entity 'molecular mechanism' named entity 'proteomic' named entity 'larvae' named entity 'culture' named entity 'transmembrane domains' named entity 'protein' named entity 'peptidase' named entity 'UniProt' named entity 'carboxypeptidase' named entity 'Biotechnology' named entity 'Myosin' named entity 'mass spectrometer' named entity 'proteomics' named entity 'acrylamide' named entity 'Southborough' named entity 'Peptide' named entity 'cuticle' named entity 'mRNA' named entity 'DMSO' named entity 'mammalian' named entity 'carboxypeptidase' named entity 'infection' named entity 'pharynx' named entity 'peptide' named entity 'Protein' named entity 'statistically significant' named entity 'antiparasitic' named entity 'biological processes' named entity 'parasite' named entity 'parasite' named entity 'living species' named entity 'electrophoresis' named entity 'molecular compounds' named entity 'protein' named entity 'anaerobic conditions' named entity 'infection' named entity 'p-value' named entity 'pharynx' named entity 'serine proteases' named entity 'helminthiases' named entity 'anthelmintics' named entity 'multiple comparison' named entity 'peptides' named entity 'protein-protein interaction' named entity 'ion channel' named entity 'peptide' named entity 'micromolar' named entity 'cuticle' named entity 'glycine receptor' named entity 'roundworms' named entity 'p-values'

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