About: Metagenomic Analysis of Fever, Thrombocytopenia and Leukopenia Syndrome (FTLS) in Henan Province, China: Discovery of a New Bunyavirus

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About: Metagenomic Analysis of Fever, Thrombocytopenia and Leukopenia Syndrome (FTLS) in Henan Province, China: Discovery of a New Bunyavirus Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Metagenomic Analysis of Fever, Thrombocytopenia and Leukopenia Syndrome (FTLS) in Henan Province, China: Discovery of a New Bunyavirus
Creator	Liu, L Ma, H Zhang, Y Huang, X Xu, B
Source	PMC
abstract	Since 2007, many cases of fever, thrombocytopenia and leukopenia syndrome (FTLS) have emerged in Henan Province, China. Patient reports of tick bites suggested that infection could contribute to FTLS. Many tick-transmitted microbial pathogens were tested for by PCR/RT-PCR and/or indirect immunofluorescence assay (IFA). However, only 8% (24/285) of samples collected from 2007 to 2010 tested positive for human granulocytic anaplasmosis (HGA), suggesting that other pathogens could be involved. Here, we used an unbiased metagenomic approach to screen and survey for microbes possibly associated with FTLS. BLASTx analysis of deduced protein sequences revealed that a novel bunyavirus (36% identity to Tehran virus, accession: HQ412604) was present only in sera from FTLS patients. A phylogenetic analysis further showed that, although closely related to Uukuniemi virus of the Phlebovirus genus, this virus was distinct. The candidate virus was examined for association with FTLS among samples collected from Henan province during 2007–2010. RT-PCR, viral cultures, and a seroepidemiologic survey were undertaken. RT-PCR results showed that 223 of 285 (78.24%) acute-phase serum samples contained viral RNA. Of 95 patients for whom paired acute and convalescent sera were available, 73 had serologic evidence of infection, with 52 seroconversions and 21 exhibiting a 4-fold increase in antibody titer to the virus. The new virus was isolated from patient acute-phase serum samples and named Henan Fever Virus (HNF virus). Whole-genome sequencing confirmed that the virus was a novel bunyavirus with genetic similarity to known bunyaviruses, and was most closely related to the Uukuniemi virus (34%, 24%, and 29% of maximum identity, respectively, for segment L, M, S at maximum query coverage). After the release of the GenBank sequences of SFTSV, we found that they were nearly identical (>99% identity). These results show that the novel bunyavirus (HNF virus) is strongly correlated with FTLS.
has issue date	2011-11-17(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1002369
bibo:pmid	22114553
has license	cc-by
sha1sum (hex)	54774da1d5cb436f9bac63726a08b399b05c6992
schema:url	https://doi.org/10.1371/journal.ppat.1002369
resource representing a document's title	Metagenomic Analysis of Fever, Thrombocytopenia and Leukopenia Syndrome (FTLS) in Henan Province, China: Discovery of a New Bunyavirus
has PubMed Central identifier	PMC3219706
has PubMed identifier	22114553
schema:publication	PLoS Pathog
resource representing a document's body	covid:54774da1d5cb436f9bac63726a08b399b05c6992#body_text
is schema:about of	named entity 'fold' named entity 'bunyavirus' named entity 'virus' named entity 'identity' named entity 'fever' named entity 'involved' named entity 'positive' named entity 'identity' named entity 'Here' named entity 'SEQUENCES' named entity 'PHASE' named entity 'CONTAINED' named entity 'CORRELATED' named entity 'QUERY' named entity 'DISTINCT' named entity 'ANALYSIS' named entity 'PAIRED' named entity 'AVAILABLE' named entity 'CANDIDATE' named entity 'PATIENT REPORTS' named entity 'CASES' named entity 'NEARLY' named entity 'SURVEY' named entity 'PCR' named entity 'PATIENT' named entity 'VIRAL' named entity 'RT-PCR' named entity 'THROMBOCYTOPENIA' named entity 'ACCESSION' named entity 'GENBANK' named entity 'TRANSMITTED' named entity 'INFECTION' named entity 'POSSIBLY' named entity 'CULTURES' named entity 'SFTSV' named entity 'GENETIC SIMILARITY' named entity 'PHLEBOVIRUS' named entity 'GENUS' named entity 'BUNYAVIRUS' named entity 'EVIDENCE OF' named entity 'VIRUS' named entity 'SEROCONVERSIONS' named entity 'ANTIBODY TITER' named entity 'GENOME SEQUENCING' named entity 'SAMPLES' named entity 'KNOWN' named entity 'PHYLOGENETIC ANALYSIS' named entity 'COLLECTED' named entity 'ASSOCIATION' named entity 'PATHOGENS' named entity 'TICK BITES' named entity 'VIRAL RNA' named entity 'UUKUNIEMI VIRUS' named entity 'HENAN' named entity 'REVEALED' named entity 'PROTEIN SEQUENCES' named entity 'HUMAN GRANULOCYTIC ANAPLASMOSIS' named entity 'ISOLATED' named entity 'RESULTS' named entity 'POSITIVE FOR' named entity 'LEUKOPENIA' named entity 'TICK' named entity 'SERA' named entity 'EXAMINED FOR' named entity 'IMMUNOFLUORESCENCE ASSAY' named entity 'FTLS' named entity 'USED' named entity 'CONTRIBUTE ' named entity 'BLASTX' named entity 'SCREEN' named entity 'HERE' named entity 'EXHIBITING' named entity 'HEALTH' named entity 'MICROBIAL PATHOGENS' named entity 'SERUM' named entity 'HAVE' named entity 'THESE'

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