About: Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus
Creator	Zhang, Ling Wu, Ying Ceciliani, Fabrizio Cheng, Anchun Wang, Mingshu Chen, Shun Jia, Renyong Liu, Mafeng Yang, Qiao Zhu, Dekang Tian, Bin Liu, Yunya Mao, Sai Yu, Yanling Zhang, Shaqiu Zhao, Xinxin Jiang, Shijin Li, Ning Huang, Juan Cai, Dongjie Deng, Liyao He, Tianqiong Ou, Xumin Wu, Liping
source	Medline; PMC
abstract	Duck plague virus (DPV) is a representative pathogen transmitted among aquatic animals that causes gross lesions and immune inhibition in geese and ducks. The mechanism of organ tropism and innate immune evasion of DPV has not been completely deciphered due to a lack of cell models to study the innate immune manipulation and pathogenicity of aquatic viruses. In the present study, we isolated five types of duck primary cells [duck embryo fibroblasts (DEFs), neurons, astrocytes, peripheral blood mononuclear cells (PBMCs), and monocytes/macrophages] to identify appropriate cell models for DPV, using tropism infection and innate immunologic assays. Cells responded differently to stimulation with DNA viruses or RNA virus analogs. DPV infection exhibited broad tropism, as the recombinant virulent strain (CHv-GFP) infected DEFs, neurons, astrocytes, and monocytes/macrophages, but not the PBMCs, as the expression of EGFP was negligible. The basal levels of innate immunity molecules were highest in monocytes/macrophages and lower in DEFs and astrocytes. Conversely, the titer and genomic copy number of the attenuated virus strain was higher in DEFs and astrocytes than in neurons and monocytes/macrophages. The titer and genomic copy number of the attenuated virus strain were higher compared with the virulent strain in DEFs, neurons, and astrocytes. The innate immune response was not significantly induced by either DPV strain in DEFs, neurons, or astrocytes. The virulent strain persistently infected monocytes/macrophages, but the attenuated strain did so abortively, and this was accompanied by the phenomenon of innate immune inhibition and activation by the virulent and attenuated strains, respectively. Blockage of IFNAR signaling promoted replication of the attenuated strain. Pre-activation of IFNAR signaling inhibited infection by the virulent strain. The selection assay results indicated that induction of innate immunity plays an essential role in controlling DPV infection, and monocytes/macrophages are an important cell model for further investigations. Our study provided practical methods for isolating and culturing duck primary cells, and our results will facilitate further investigations of organ tropism, innate immune responses, latent infection, and the effectiveness of antiviral drugs for treating DPV and potentially other aerial bird pathogens.
has issue date	2020-01-28(xsd:dateTime)
bibo:doi	10.3389/fimmu.2019.03131
bibo:pmid	32063900
has license	cc-by
sha1sum (hex)	5503464f5c8ac4db987ce87b05f224a7aaafc1a7
schema:url	https://doi.org/10.3389/fimmu.2019.03131
resource representing a document's title	Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus
has PubMed Central identifier	PMC6999086
has PubMed identifier	32063900
schema:publication	Front Immunol
resource representing a document's body	covid:5503464f5c8ac4db987ce87b05f224a7aaafc1a7#body_text
is schema:about of	named entity 'monocytes' named entity 'assay' named entity 'DPV' named entity 'lesions' named entity 'astrocytes' named entity 'immunity' named entity 'strain' named entity 'attenuated' named entity 'aquatic' named entity 'strain' named entity 'types' named entity 'virulent' named entity 'SELECTION' named entity 'NEURONS' named entity 'PRIMARY CELLS' named entity 'IMMUNOLOGIC ASSAYS' named entity 'PERSISTENTLY' named entity 'PLAYS' named entity 'IFNAR' named entity 'INHIBITION' named entity 'NUMBER OF' named entity 'PBMCS' covid:arg/5503464f5c8ac4db987ce87b05f224a7aaafc1a7 named entity 'BUT' named entity 'MECHANISM' named entity 'ACTIVATION' named entity 'AQUATIC' named entity 'PATHOGEN' named entity 'APPROPRIATE' named entity 'REPLICATION' named entity 'DUE TO' named entity 'ISOLATED' named entity 'RESULTS' named entity 'PRIMARY CELLS' named entity 'STRAINS' named entity 'MANIPULATION' named entity 'TITER' named entity 'PATHOGENICITY' named entity 'PRE' named entity 'BLOCKAGE' named entity 'RECOMBINANT' named entity 'LEVELS' named entity 'IMMUNOLOGIC CELL' named entity 'DUCK' named entity 'ISOLATION' named entity 'VIRUSES' named entity 'COMPLETELY' named entity 'USING' named entity 'PATHOGENIC' named entity 'MODELS' named entity 'DUCK PLAGUE VIRUS' named entity 'EITHER' named entity 'INNATE IMMUNITY' named entity 'AQUATIC ANIMALS' named entity 'VIRULENT STRAIN' named entity 'TRANSMITTED' named entity 'TO IDENTIFY' named entity 'COPY NUMBER'

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