About: Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase

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About: Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase
Creator	Gabridge, Michael Gladd, Martha Lundin, Daniel
Source	PMC
abstract	An enzyme-linked immunosorbent assay (ELISA) was developed in order to serve in detecting and speciating mycoplasmas isolated from cell cultures. Its main features included a biotin-streptavidin amplification step and a solid phase consisting of a microporous membrane. Cell samples in the form of suspensions were applied to nitrocellulose or ion exchange membranes immobilized in commerciallyavailable microtiter, multiwell manifolds. The blocking buffer contained 1% purified α-casein. The primary antibodies were monoclonal and the polyclonal secondary antibody was biotinylated. The enzyme utilized was streptavidin-horseradish peroxidase. The substrate-dye complex consisted of either 4-chloro-1-naphthol and hydrogen peroxide or ortho phenylene diamine (OPD) and hydrogen peroxide. The presence of homologous antiserum in the reaction sequence gave clearly visible, colored reactions on the membrane when 50 ul with approximately 10(5) or more cfu/ml were present. This new biotin-avidin microporous membrane (BAMM-ELISA) test can be used both to detect mycoplasmas and to speciate them. The BAMM-ELISA is simple, rapid, sensitive, specific and economical. As such, it has potential for aiding in the control of mycoplasma contamination in cell culture, and could prove useful in clinical diagnostic applications as well.
has issue date	1986-01-01(xsd:dateTime)
bibo:doi	10.1007/bf02623451
bibo:pmid	3733642
has license	no-cc
sha1sum (hex)	5585c599fcceaaabc7f20651e8b71b08fe39cf3e
schema:url	https://doi.org/10.1007/bf02623451
resource representing a document's title	Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase
has PubMed Central identifier	PMC7089076
has PubMed identifier	3733642
schema:publication	In Vitro Cell Dev Biol
resource representing a document's body	covid:5585c599fcceaaabc7f20651e8b71b08fe39cf3e#body_text
is schema:about of	named entity 'potential' named entity 'rapid' named entity 'features' named entity 'blocking' named entity 'CONTAINED' named entity 'COLORED' named entity 'ITS' named entity 'CASEIN' named entity 'RAPID' named entity 'USEFUL' named entity 'INCLUDED' named entity 'MAIN' named entity 'HOMOLOGOUS' named entity 'PRESENCE OF' named entity 'PURIFIED' named entity 'reaction' named entity 'peroxidase' named entity 'complex' named entity 'diamine' named entity 'secondary antibody' named entity 'AND' named entity 'enzyme-linked immunosorbent assay' named entity 'hydrogen peroxide' named entity 'polyclonal' named entity 'nitrocellulose' named entity 'primary antibodies' named entity 'streptavidin' named entity 'biotinylated' named entity 'mycoplasma' named entity 'antiserum' named entity 'BIOTIN' named entity 'IgG' named entity 'binding sites' named entity 'fluorochrome' named entity 'left column' named entity 'substrates' named entity 'mycoplasmas' named entity 'fluorescein' named entity 'biotin' named entity 'early 1970' named entity 'casein' named entity 'avidin' named entity 'antigen' named entity 'contaminant' named entity 'casein' named entity 'densitometric' named entity 'PBS' named entity 'biotin' named entity 'mycoplasma' named entity 'hybridoma' named entity 'binding constant' named entity 'enzyme' named entity 'non-specific' named entity 'ELISA assay' named entity 'mycoplasma' named entity 'inoculum' named entity 'nitrocellulose' named entity 'thymic' named entity 'enzyme' named entity 'nitrocellulose' named entity 'chart recorder' named entity 'serology' named entity 'Gaithersburg' named entity 'microtiter plates' named entity 'membrane filter' named entity 'biotinylated' named entity 'cell culture' named entity 'Acholeplasma' named entity 'mycoplasmas' named entity 'Mycoplasmas' named entity 'antisera' named entity 'antibody' named entity 'antigen' named entity 'antigen' named entity 'arginine' named entity 'antisera' named entity 'methanol' named entity 'antigen-antibody reaction' named entity '1-naphthol'

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