About: Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F

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About: Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F
Creator	Van Bergen En Henegouwen, Paul Doulkeridou, Sofia Gros, Piet Kroon-Batenburg, Loes Lutz, Martin Manshande, Jip Neviani, Viviana Oosterheert, Wout Pearce, Nicholas Pos, Wouter Xenaki, Katerina
Source	BioRxiv
abstract	Tetraspanins are ubiquitous eukaryotic membrane proteins that contribute to a variety of signaling processes by spatially organizing partner-receptor molecules in the plasma membrane. How tetraspanins bind and cluster partner receptors into so-called tetraspanin-enriched microdomains is unknown. Here we present crystal structures of the large extracellular loop of CD9 in complex with nanobodies 4C8 and 4E8; and, the cryo-EM structure of 4C8-bound CD9 in complex with its prototypical partner EWI-F. The CD9 - EWI-F complex displays a tetrameric arrangement with two centrally positioned EWI-F molecules, dimerized through their ectodomains, and two CD9 molecules, one bound to each EWI-F single-pass transmembrane helix through CD9-helices h3 and h4. In the crystal structures, nanobodies 4C8 and 4E8 bind CD9 at the C and D loop, in agreement with 4C8 binding at the ends of the CD9 - EWI-F cryo-EM complex. Overall, the 4C8 - CD9 - EWI-F - EWI-F - CD9 - 4C8 complexes varied from nearly two-fold symmetric (i.e. with the two CD9 - 4C8 copies in nearly anti-parallel orientation) to ca. 50° bent arrangements. Since membrane helices h1 and h2 and the EC2 D-loop have been previously identified as sites for tetraspanin homo-dimerization, the observed linear but flexible arrangement of CD9 - EWI-F with potential CD9 - CD9 homo-dimerization at either end provides a new ‘concatenation model’ for forming short linear or circular assemblies, which may explain the occurrence of tetraspanin-enriched microdomains.
has issue date	2020-06-03(xsd:dateTime)
bibo:doi	10.1101/2020.06.02.130047
has license	biorxiv
sha1sum (hex)	55e629e14908343aefb1479380a8dce6f07fbd84
schema:url	https://doi.org/10.1101/2020.06.02.130047
resource representing a document's title	Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F
schema:publication	bioRxiv
resource representing a document's body	covid:55e629e14908343aefb1479380a8dce6f07fbd84#body_text
is schema:about of	named entity 'model' named entity 'EC2' named entity 'ends' named entity 'D-loop' named entity 'linear' named entity 'orientation' named entity 'positioned' named entity 'CD9' named entity 'observed' named entity 'arrangements' named entity 'bind' named entity 'Overall' named entity 'arrangement' named entity 'linear' named entity 'cryo-EM' named entity 'CD9' named entity 'complex' named entity 'CD9' named entity 'CD9' named entity 'kDa' named entity 'EWI' named entity 'tetramer' named entity 'C-terminal' named entity 'cell cultures' named entity 'CD9' named entity 'EWI' named entity 'TMDs' named entity 'nanobodies' named entity 'expression vector' named entity 'image processing' named entity 'fluorescence' named entity 'Strep' named entity 'co-purified' named entity 'PCR' named entity 'Tetraspanin' named entity 'cryo-EM' named entity 'CD9' named entity 'conformational change' named entity 'EWI' named entity 'eluted' named entity 'EWI' named entity 'Nyquist frequency' named entity 'Ig-like' named entity 'hydrophobic interaction' named entity 'CD9' named entity 'EWI' named entity 'micelle' named entity 'EWI' named entity 'integrins' named entity 'cloned' named entity 'EWI' named entity 'digitonin' named entity 'HEK293' named entity 'CD9' named entity 'colon' named entity 'tetraspanin' named entity 'EWI' named entity 'Ig-like domains' named entity '37°C' named entity 'digitonin' named entity 'EWI' named entity 'Protein' named entity 'tryptophan' named entity 'nanobody' named entity 'Trp' named entity 'PHASER' named entity 'R101' named entity 'structural model' named entity 'w/v' named entity 'CD9' named entity 'CD81' named entity 'CD9' named entity 'Nanobodies' named entity 'CD81'

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