About: Human Coronavirus EMC Does Not Require the SARS-Coronavirus Receptor and Maintains Broad Replicative Capability in Mammalian Cell Lines

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About: Human Coronavirus EMC Does Not Require the SARS-Coronavirus Receptor and Maintains Broad Replicative Capability in Mammalian Cell Lines Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human Coronavirus EMC Does Not Require the SARS-Coronavirus Receptor and Maintains Broad Replicative Capability in Mammalian Cell Lines
Creator	Fouchier, Ron Osterhaus, Albert Drosten, Christian Meyer, Benjamin Wollny, Robert Binger, Tabea Eckerle, Isabella Kallies, Stephan Muth, Doreen Specht, Sabine Suliman, Tasnim Tschapka, Marco Zimmermann, Katrin Haagmans, Bart Bestebroer, Theo Müller, Marcel Buchmeier, Michael Smits, Saskia Stalin Raj, V Zaki, Ali
Source	Medline; PMC
abstract	A new human coronavirus (hCoV-EMC) has emerged very recently in the Middle East. The clinical presentation resembled that of the severe acute respiratory syndrome (SARS) as encountered during the epidemic in 2002/2003. In both cases, acute renal failure was observed in humans. HCoV-EMC is a member of the same virus genus as SARS-CoV but constitutes a sister species. Here we investigated whether it might utilize angiotensin-converting enzyme 2 (ACE2), the SARS-CoV receptor. Knowledge of the receptor is highly critical because the restriction of the SARS receptor to deep compartments of the human respiratory tract limited the spread of SARS. In baby hamster kidney (BHK) cells, lentiviral transduction of human ACE2 (hACE2) conferred permissiveness and replication for SARS-CoV but not for hCoV-EMC. Monkey and human kidney cells (LLC-MK2, Vero, and 769-P) and swine kidney cells were permissive for both viruses, but only SARS-CoV infection could be blocked by anti-hACE2 antibody and could be neutralized by preincubation of virus with soluble ACE2. Our data show that ACE2 is neither necessary nor sufficient for hCoV-EMC replication. Moreover, hCoV-EMC, but not SARS-CoV, replicated in cell lines from Rousettus, Rhinolophus, Pipistrellus, Myotis, and Carollia bats, representing four major chiropteran families from both suborders. As human CoV normally cannot replicate in bat cells from different families, this suggests that hCoV-EMC might use a receptor molecule that is conserved in bats, pigs, and humans, implicating a low barrier against cross-host transmission.
has issue date	2012-12-11(xsd:dateTime)
bibo:doi	10.1128/mbio.00515-12
bibo:pmid	23232719
has license	cc-by-nc-sa
sha1sum (hex)	5650690daf962117b9831c42178ffd6a6a969300
schema:url	https://doi.org/10.1128/mbio.00515-12
resource representing a document's title	Human Coronavirus EMC Does Not Require the SARS-Coronavirus Receptor and Maintains Broad Replicative Capability in Mammalian Cell Lines
has PubMed Central identifier	PMC3520110
has PubMed identifier	23232719
schema:publication	mBio
resource representing a document's body	covid:5650690daf962117b9831c42178ffd6a6a969300#body_text
is schema:about of	named entity 'HUMAN CORONAVIRUS' named entity 'ACUTE RENAL FAILURE' named entity 'HERE' named entity 'EPIDEMIC' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'RECENTLY' named entity 'HUMANS' named entity 'IS A' named entity 'INVESTIGATED' named entity 'OBSERVED' named entity 'HUMAN CORONAVIRUS' named entity 'CLINICAL PRESENTATION' named entity 'BUT' named entity 'Here' named entity 'emerged' named entity 'SARS' named entity 'BROAD' covid:arg/5650690daf962117b9831c42178ffd6a6a969300 named entity 'NEW' named entity 'MAMMALIAN CELL' named entity 'CORONAVIRUS RECEPTOR' named entity 'CELL LINES' named entity 'DOES NOT' named entity 'SARS-COV' named entity 'CASES' named entity 'GENUS' named entity 'MEMBER OF' named entity 'RECEPTOR' named entity 'CAPABILITY' named entity 'EMC' named entity 'SISTER' named entity 'MIDDLE EAST' named entity 'EMC' named entity 'VIRUS' named entity 'ANGIOTENSIN-CONVERTING ENZYME 2' named entity 'SPECIES' named entity 'human' named entity 'severe acute respiratory syndrome (SARS)' named entity 'member' named entity 'investigated' named entity 'Middle East' named entity 'ACE2' named entity 'receptor' named entity 'HCoV-EMC' named entity 'SARS-CoV' named entity 'acute renal failure' named entity 'Middle East' named entity 'Coronavirus' named entity 'Mammalian Cell' named entity 'Human Coronavirus' named entity 'protein' named entity 'bat' named entity 'respiratory disease' named entity 'bat' named entity 'cell lines' named entity 'infection' named entity 'downregulation' named entity 'London' named entity 'SARS' named entity 'SARS-CoV' named entity 'lung' named entity 'virus'

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