About: Replication of sialodacryoadenitis virus in mouse L-2 cells

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About: Replication of sialodacryoadenitis virus in mouse L-2 cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Replication of sialodacryoadenitis virus in mouse L-2 cells
Creator	Percy, D Bond, S Macinnes, J
Source	Medline; PMC
abstract	Sialodacryoadenitis (SDA) is a naturally-occurring infection of the laboratory rat raused by the coronavirus, sialodacryoadenitis virus (SDAV). The study of SDAV has been limited because there is no widely available continuous cell line for the propagation of high titers of the virus. The purpose of this study, therefore, was to compare the ability of SDAV to replicate in the permanent cell lines, LBC, of rat origin, and the mouse cell lines. L-929 and L-2. Following 2 to 6 repeated passages of SDAV in LBC cells, the virus could be readily propagated in LBC and L-2 cells, but not in L-929 cells. Similarly, SDAV adapted to replicate directly in L-2 cells could be readily propagated in LBC, but not L-929 cells. In LBC and L-2 cells, cytopathic effect (CPE), viral antigen, viral particles, and virus infectivity could be demonstrated. Titers of up to 10(8.0) infectious viral particles/0.25 ml of culture fluid were obtained at 48 hours in L-2 cells. Titers in LBC cells were one to two logs lower. When susceptible rats were inoculated with eighth passage L-2 cell-adapted virus, they developed typical lesions of SDA. Virus could be recovered from infected tissues and propagated in L-2 cells on first passage. The ability to propagate SDAV to high titers in the widely available L-2 cell line should promote the study of this virus and facilitate its comparison with other murine coronaviruses.
has issue date	1989-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01315553
bibo:pmid	2539798
has license	no-cc
sha1sum (hex)	566ed6f31a1b30b9630496aed6914525e3867359
schema:url	https://doi.org/10.1007/bf01315553
resource representing a document's title	Replication of sialodacryoadenitis virus in mouse L-2 cells
has PubMed Central identifier	PMC7086637
has PubMed identifier	2539798
schema:publication	Arch Virol
resource representing a document's body	covid:566ed6f31a1b30b9630496aed6914525e3867359#body_text
is schema:about of	named entity 'viral' named entity 'viral' named entity 'infection' named entity 'cells' named entity 'eighth' named entity 'LBC' named entity 'susceptible' named entity '48 HOURS' named entity 'L-2' named entity 'VIRUS INFECTIVITY' named entity 'BUT' named entity 'SDA' named entity 'L-2' named entity 'PERMANENT' named entity 'LIMITED' named entity 'REPEATED' named entity 'AVAILABLE' named entity 'SUSCEPTIBLE' named entity 'STUDY' named entity 'REPLICATE' named entity 'LOGS' named entity 'CULTURE FLUID' named entity 'CELL LINES' named entity 'COULD BE' named entity 'INFECTIOUS' named entity 'INFECTION' named entity 'VIRUS' named entity 'TO COMPARE' named entity 'TISSUES' named entity 'LABORATORY RAT' named entity 'MOUSE CELL' named entity 'VIRAL PARTICLES' named entity 'CORONAVIRUSES' named entity 'COMPARISON' named entity 'REPLICATION' named entity 'CELLS' named entity 'MOUSE' named entity 'SIALODACRYOADENITIS VIRUS' named entity 'CONTINUOUS' named entity 'IS A' named entity 'ITS' named entity 'ORIGIN' named entity '108' named entity 'FOLLOWING' named entity 'CORONAVIRUS' named entity 'EIGHTH' named entity 'RAT' named entity 'HIGH' named entity 'ABILITY' named entity 'VIRAL ANTIGEN' named entity 'SIALODACRYOADENITIS' named entity 'RECOVERED' named entity 'TYPICAL' named entity 'OBTAINED' named entity 'LBC' named entity 'RATS' named entity 'LOWER' named entity 'INFECTED' named entity 'LESIONS' named entity 'DIRECTLY' named entity 'CELLS' named entity 'SIALODACRYOADENITIS VIRUS' named entity 'PROMOTE' named entity 'CELL LINE' named entity 'ADAPTED' named entity 'PURPOSE' named entity 'MURINE' named entity 'UP TO' named entity 'DEMONSTRATED' named entity 'PASSAGE' named entity 'CYTOPATHIC EFFECT' named entity 'PROPAGATION' named entity 'L-929' covid:arg/566ed6f31a1b30b9630496aed6914525e3867359 named entity 'laboratory rat' named entity 'When' named entity 'tissues' named entity 'virus' named entity 'cell lines'

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