About: Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks

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About: Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks
Creator	Benavides, Julio Fernández-Escobar, Mercedes Ortega-Mora, Luis Calero-Bernal, Rafael Collantes-Fernández, Esther Guerrero-Molina, Cristina Gutiérrez-Expósito, Daniel Regidor-Cerrillo, Javier
Source	PMC
abstract	BACKGROUND: Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain. METHODS: Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes. RESULTS: As a result, 30 isolates were obtained from 9 Spanish regions: 10 isolates from abortion-derived samples and 20 isolates from adult myocardial tissues. Overall, 3 genotypes were found: ToxoDB#3 (type II PRU variant) in 90% (27/30) of isolates, ToxoDB#2 (clonal type III) in 6.7% (2/30), and ToxoDB#1 (clonal type II) in 3.3% (1/30). When T. gondii-positive tissue samples (n = 151) were directly subjected to RFLP genotyping, complete restriction profiles were obtained for 33% of samples, and up to 98% of the specimens belonged to the type II PRU variant. A foetal brain showed a clonal type II pattern, and four specimens showed unexpected type I alleles at the SAG3 marker, including two foetal brains that showed I + II alleles as co-infection events. Amplicons of SAG3, GRA6 and GRA7 obtained from isolates and clinical samples were subjected to sequencing, allowing us to confirm RFLP results and to detect different single-nucleotide polymorphisms. CONCLUSIONS: The present study informed the existence of a predominant type II PRU variant genotype (ToxoDB#3) infecting domestic sheep in Spain, in both abortion cases and chronic infections in adults, coexisting with other clonal (ToxoDB#1 and ToxoDB#2), much less frequent genotypes, as well as polymorphic strains as revealed by clinical sample genotyping. The use of multilocus sequence typing aided in accurately estimating T. gondii intragenotype diversity. [Image: see text]
has issue date	2020-08-05(xsd:dateTime)
bibo:doi	10.1186/s13071-020-04275-z
has license	cc-by
sha1sum (hex)	57b21b509ac88f0c0c7bb7f4f77a27fd4b5c3393
schema:url	https://doi.org/10.1186/s13071-020-04275-z
resource representing a document's title	Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks
has PubMed Central identifier	PMC7404076
schema:publication	Parasit Vectors
resource representing a document's body	covid:57b21b509ac88f0c0c7bb7f4f77a27fd4b5c3393#body_text
is schema:about of	named entity 'Toxoplasma' named entity 'consumed' named entity 'sheep' named entity 'RISK' named entity 'SPAIN' named entity 'ovine' named entity 'Isolation' named entity 'abortion' named entity 'Spain' named entity 'evolutionary relationships' named entity 'bioassays' named entity 'SNP' named entity 'toxoplasmosis' named entity 'sheep' named entity 'non-clonal' named entity '20/50' named entity 'genotyping' named entity 'ovine' named entity 'Europe' named entity 'Lonza Group' named entity 'PCR' named entity 'sample size' named entity 'sheep' named entity 'Evans Blue' named entity 'bioassay' named entity 'sequence homology' named entity 'FITC' named entity 'genetic diversity' named entity 'T. gondii' named entity 'polymorphic' named entity 'histological' named entity 'IgG antibody' named entity 'nested PCR' named entity 'double peak' named entity 'genotypes' named entity 'abortion' named entity 'geographical distribution' named entity 'Sigma-Aldrich' named entity 'abortion' named entity 'alleles' named entity 'allele' named entity 'genetic analysis' named entity 'T. gondii' named entity 'SNP' named entity 'abortion' named entity 'mice' named entity 'bradyzoites' named entity 'alleles' named entity 'Palencia Province' named entity 'Bioassay' named entity 'homology' named entity 'serum' named entity 'allele' named entity 'myocardial tissue' named entity 'GenBank' named entity 'abortion' named entity 'Badajoz Province' named entity 'ovine' named entity 'Italy' named entity 'Europe' named entity 'Peritoneal' named entity 'toxoplasmosis' named entity 'abortion' named entity 'co-infection' named entity 'genotype' named entity 'sheep' named entity 'genotypes' named entity 'sheep' named entity 'mice' named entity 'Spain' named entity 'Spain' named entity 'abortion' named entity 'T. gondii' named entity 'pregnancy' named entity 'ovine' named entity 'Phylogenetic analyses'

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