About: Development of TaqMan(® )MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1

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About: Development of TaqMan(® )MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of TaqMan(® )MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1
Creator	Cheng, Anchun Wang, Mingshu Chen, Shun Guo, Yufei Jia, Renyong Shen, Chanjuan Zhang, Na
source	PMC
abstract	BACKGROUND: Anatid herpesvirus 1 (AHV-1) is an alphaherpesvirus associated with latent infection and mortality in ducks and geese and is currently affecting the world-wide waterfowl production severely. Here we describe a fluorescent quantitative real-time PCR (FQ-PCR) method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology. RESULTS: The detection limit of the assay was 1 × 10(1 )standard DNA copies, with a sensitivity of 2 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intra-assay and inter-assay coefficients of variation. CONCLUSION: The high sensitivity, specificity, simplicity and reproducibility of the AHV-1 fluorogenic PCR assay, combined with its wide dynamic range and high throughput, make this method suitable for a broad spectrum of AHV-1 etiologically related application.
has issue date	2009-06-04(xsd:dateTime)
bibo:doi	10.1186/1743-422x-6-71
bibo:pmid	19497115
has license	cc-by
sha1sum (hex)	5813a786b66940f45bd1083081448cc53edcadd4
schema:url	https://doi.org/10.1186/1743-422x-6-71
resource representing a document's title	Development of TaqMan(® )MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1
has PubMed Central identifier	PMC2696427
has PubMed identifier	19497115
schema:publication	Virol J
resource representing a document's body	covid:5813a786b66940f45bd1083081448cc53edcadd4#body_text
is schema:about of	named entity 'anatid' named entity 'SEVERELY' named entity 'MORTALITY' named entity 'MGB' named entity 'TECHNOLOGY' named entity 'PCR' named entity 'HERE' named entity 'TAQMAN' named entity 'ANATID HERPESVIRUS 1' named entity 'ALPHAHERPESVIRUS' named entity 'GEESE' named entity 'ANATID HERPESVIRUS 1' named entity 'ASSOCIATED WITH' named entity 'PCR ASSAY' named entity 'DEVELOPMENT' named entity 'REAL-TIME PCR ' named entity 'MEASUREMENT' named entity 'AFFECTING' named entity 'PRODUCTION' named entity 'CURRENTLY' named entity 'MGB' named entity 'WATERFOWL' named entity 'BASED' named entity 'WIDE' named entity 'FLUORESCENT' named entity 'DUCKS' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'DESCRIBE' named entity 'TAQMAN' named entity 'FAST' named entity 'LATENT INFECTION' named entity 'REAL-TIME PCR ' named entity 'WORLD' named entity 'METHOD' named entity 'BACKGROUND' named entity 'FLUORESCENT' named entity 'DETECTION' named entity 'JOURNAL ' named entity 'VIROLOGY' named entity 'DNA' named entity 'AHV-1' covid:arg/5813a786b66940f45bd1083081448cc53edcadd4 named entity 'TaqMan' named entity 'MGB' named entity 'geese' named entity 'mortality' named entity 'Development' named entity 'Virology Journal' named entity 'assay' named entity 'Primer' named entity 'quencher' named entity 'TaqMan' named entity 'PCR' named entity 'PCR' named entity 'standard curve' named entity 'hybridized' named entity 'ileum' named entity 'lung' named entity 'standard curve' named entity 'μmol/L' named entity 'plasmid' named entity 'E.coli' named entity 'quencher' named entity 'electrophoresis' named entity 'infection' named entity 'Bursa of Fabricius' named entity 'kidney' named entity 'fibroblast' named entity 'dye' named entity 'PCR' named entity 'quarantine' named entity 'waterfowl' named entity 'virulent strain' named entity 'Takara Bio' named entity 'virus'

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