About: Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR

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About: Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR
Creator	Wang, Wei Gonzalez, Richard Li, Yongjun Vernet, Guy Wang, Jianwei Huang, Fang Jin, Qi Yang, Yaowu
source	Elsevier; Medline; PMC
abstract	Pandemic influenza A/H1N1 2009 and seasonal influenza viruses are currently co-circulating worldwide. A rapid, sensitive, and specific assay for distinguishing pandemic influenza A/H1N1 2009 from seasonal influenza viruses and for subtyping seasonal influenza A viruses could aid in the surveillance and control of these viral infections. Here, such a multiplex real-time RT-PCR (rRT-PCR) assay for typing influenza A and B viruses and the pandemic influenza A/H1N1 2009 is developed. This assay can also subtype seasonal influenza A viruses simultaneously. The analytical sensitivity is 10–10(4) copies/reaction. The coefficients of variation of inter-assay and intra-assay are 0.04–0.45% and 0.08–0.97%, respectively. The new multiplex rRT-PCR assay is more sensitive in subtyping seasonal influenza viruses than the conventional PCR techniques. Results obtained with this assay for the detection of pandemic influenza A/H1N1 2009 are highly consistent (96.88%) with those achieved using the US CDC's rRT-PCR protocol. A sample identified as “pandemic influenza A/H1N1 2009 positive” by the US CDC's rRT-PCR was reclassified correctly as subtype H3N2 using this assay. Taken together, this new multiplex rRT-PCR protocol could be an important tool for improving diagnosis and management of the pandemic influenza A/H1N1 2009 and seasonal influenza viruses.
has issue date	2010-03-19(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.03.007
bibo:pmid	20304017
has license	no-cc
sha1sum (hex)	587e11d9868ac5731428c8ea73f11827d63382f3
schema:url	https://doi.org/10.1016/j.jviromet.2010.03.007
resource representing a document's title	Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR
has PubMed Central identifier	PMC7112828
has PubMed identifier	20304017
schema:publication	J Virol Methods
resource representing a document's body	covid:587e11d9868ac5731428c8ea73f11827d63382f3#body_text
is schema:about of	named entity 'SIMULTANEOUS' named entity 'VIRUSES' named entity 'INFLUENZA A' named entity 'REAL-TIME RT-PCR' named entity 'PANDEMIC' named entity 'INCLUDING' named entity 'MULTIPLEX' named entity 'TYPING' named entity 'lysis buffer' named entity 'H1N1' named entity 'fluorescence' named entity 'PCR' named entity 'influenza A and B viruses' named entity 'real-time RT-PCR' named entity 'influenza A/H1N1' named entity 'primers' named entity 'Centers for Disease Control and Prevention' named entity 'subtyping' named entity 'seasonal influenza' named entity 'influenza' named entity 'fluorochrome' named entity 'influenza' named entity 'rRT-PCR' named entity 'H1N1' named entity 'virus' named entity 'nucleic acid' named entity 'sequenced' named entity 'reproducibility' named entity 'primers' named entity 'H1N1' named entity 'H3N2' named entity 'LODs' named entity 'primers' named entity 'Elsevier' named entity 'rRT-PCR' named entity 'viruses' named entity 'rRT-PCR' named entity 'influenza A/H1N1' named entity 'Statistical analysis' named entity 'conserved regions' named entity 'cloned' named entity 'rRT-PCR' named entity 'randomly selected' named entity 'Daum' named entity '300 nM' named entity 'viruses' named entity 'H3N2' named entity 'RT-PCR' named entity 'rRT-PCR' named entity 'RNA' named entity 'viruses' named entity 'influenza A/H1N1' named entity 'Influenza Virus' named entity 'influenza A/H1N1' named entity 'LODs' named entity 'Primer' named entity 'influenza A virus' named entity 'US CDC' named entity 'rRT-PCR' named entity 'OC43' named entity 'adenovirus' named entity 'US CDC' named entity 'Flu' named entity 'fluorescence' named entity 'quencher' named entity 'adenovirus' named entity 'A/H1N1' named entity 'rRT-PCR' named entity 'influenza A/H1N1' named entity 'randomly selected' named entity 'virus' named entity 'viruses' named entity 'influenza' named entity 'CDC'

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