About: Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins

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About: Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins
Creator	Nior, Jú Faria, Matheus Filho, Hercílio Mineo, José Mineo, Tiago Mota, Caroline Santiago, Fernanda Silva, Deise Silva, Murilo Cunha-Jú, Jair Ferreira, B Ferreira, Lvaro Macê, Arlindo
source	PMC
abstract	BACKGROUND: Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to Fc mammalian receptors. IgY antibodies avoid those limitations; therefore they could be an alternative to be applied in T. gondii model. METHODOLOGY/PRINCIPAL FINDINGS: In this study we immunized hens with soluble tachyzoite antigens of T. gondii (STAg) and purified egg yolk antibodies (IgY) by an inexpensive and simple method, with high yield and purity degree. IgY anti-STAg antibodies presented high avidity and were able to recognize a broad range of parasite antigens, although some marked differences were observed in reactivity profile between antibodies produced in immunized hens and mice. Interestingly, IgY antibodies against Neospora caninum and Eimeria spp. did not react to STAg. We also show that IgY antibodies were suitable to detect T. gondii forms in paraffin-embedded sections and culture cell monolayers. CONCLUSIONS/SIGNIFICANCE: Due to its cost-effectiveness, high production yield and varied range of possible applications, polyclonal IgY antibodies are useful tools for studies involving T. gondii.
has issue date	2012-07-12(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0040391
bibo:pmid	22808150
has license	cc-by
sha1sum (hex)	58f93bf42d2c4e21ed8effe492719d992849eff6
schema:url	https://doi.org/10.1371/journal.pone.0040391
resource representing a document's title	Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins
has PubMed Central identifier	PMC3395712
has PubMed identifier	22808150
schema:publication	PLoS One
resource representing a document's body	covid:58f93bf42d2c4e21ed8effe492719d992849eff6#body_text
is schema:about of	named entity 'limitations' named entity 'complement activation' named entity 'study' named entity 'Characterization' named entity 'TOXOPLASMA GONDII' named entity 'ANIMAL' named entity 'BACKGROUND' named entity 'IMMUNIZED' named entity 'DIFFERENT' named entity 'AFFINITY' named entity 'OCULAR' named entity 'APPROACHES' named entity 'WARM' named entity 'SERA' named entity 'DIAGNOSIS' named entity 'ITS' named entity 'BLEEDING' named entity 'RECEPTORS' named entity 'COULD BE' named entity 'IGY' named entity 'INTERFERENCE' named entity 'BLOOD' named entity 'ALTERNATIVE' named entity 'MODEL' named entity 'COMPLEMENT ACTIVATION' named entity 'PRESENT' named entity 'LOW YIELD' named entity 'CHARACTERIZATION' named entity 'IMMUNOGLOBULINS' named entity 'EGG YOLK' named entity 'CHICKEN EGG' named entity 'NECESSITY' named entity 'AVOID' named entity 'LIMITATIONS' named entity 'MAMMALIAN' named entity 'HOSTS' named entity 'TOXOPLASMA GONDII' named entity 'SPECIFIC' named entity 'PRODUCTION' named entity 'APPLICATIONS' named entity 'POLYCLONAL' named entity 'MAMMALS' named entity 'APPLIED' named entity 'STUDY' named entity 'USED' named entity 'NEUROLOGICAL DISORDERS' named entity 'ABORTIONS' named entity 'CAUSE' named entity 'WIDE' named entity 'INCLUDING' named entity 'INTERACTIONS' named entity 'ANTIBODIES' named entity 'SOURCE' named entity 'PRODUCTION' named entity 'RHEUMATOID FACTOR' covid:arg/58f93bf42d2c4e21ed8effe492719d992849eff6 named entity 'antibodies' named entity 'diagnosis' named entity 'abortions' named entity 'interactions' named entity 'Unfortunately' named entity 'mammals' named entity 'hyperimmune' named entity 'T. gondii' named entity 'Immunoglobulins' named entity 'Eimeria acervulina' named entity 'production animals' named entity 'parasites' named entity 'cell nucleus'

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