About: Mutations in the Fusion Protein Cleavage Site of Avian Paramyxovirus Serotype 4 Confer Increased Replication and Syncytium Formation In Vitro but Not Increased Replication and Pathogenicity in Chickens and Ducks

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About: Mutations in the Fusion Protein Cleavage Site of Avian Paramyxovirus Serotype 4 Confer Increased Replication and Syncytium Formation In Vitro but Not Increased Replication and Pathogenicity in Chickens and Ducks Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Mutations in the Fusion Protein Cleavage Site of Avian Paramyxovirus Serotype 4 Confer Increased Replication and Syncytium Formation In Vitro but Not Increased Replication and Pathogenicity in Chickens and Ducks
Creator	Kim, Shin-Hee Shive, Heather Xiao, Sa Collins, Peter Samal, Siba
source	PMC
abstract	To evaluate the role of the F protein cleavage site in the replication and pathogenicity of avian paramyxoviruses (APMVs), we constructed a reverse genetics system for recovery of infectious recombinant APMV-4 from cloned cDNA. The recovered recombinant APMV-4 resembled the biological virus in growth characteristics in vitro and in pathogenicity in vivo. The F cleavage site sequence of APMV-4 (DIQPR↓F) contains a single basic amino acid, at the -1 position. Six mutant APMV-4 viruses were recovered in which the F protein cleavage site was mutated to contain increased numbers of basic amino acids or to mimic the naturally occurring cleavage sites of several paramyxoviruses, including neurovirulent and avirulent strains of NDV. The presence of a glutamine residue at the -3 position was found to be important for mutant virus recovery. In addition, cleavage sites containing the furin protease motif conferred increased replication and syncytium formation in vitro. However, analysis of viral pathogenicity in 9-day-old embryonated chicken eggs, 1-day-old and 2-week-old chickens, and 3-week-old ducks showed that none the F protein cleavage site mutations altered the replication, tropism, and pathogenicity of APMV-4, and no significant differences were observed among the parental and mutant APMV-4 viruses in vivo. Although parental and mutant viruses replicated somewhat better in ducks than in chickens, they all were highly restricted and avirulent in both species. These results suggested that the cleavage site sequence of the F protein is not a limiting determinant of APMV-4 pathogenicity in chickens and ducks.
has issue date	2013-01-14(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0050598
bibo:pmid	23341874
has license	cc0
sha1sum (hex)	59a11e8976b666ba112da942d7ba7c698d06a9e8
schema:url	https://doi.org/10.1371/journal.pone.0050598
resource representing a document's title	Mutations in the Fusion Protein Cleavage Site of Avian Paramyxovirus Serotype 4 Confer Increased Replication and Syncytium Formation In Vitro but Not Increased Replication and Pathogenicity in Chickens and Ducks
has PubMed Central identifier	PMC3544850
has PubMed identifier	23341874
schema:publication	PLoS One
resource representing a document's body	covid:59a11e8976b666ba112da942d7ba7c698d06a9e8#body_text
is schema:about of	named entity 'SYNCYTIUM FORMATION' named entity 'observed' named entity 'recombinant' named entity 'virus' named entity 'replicated' named entity 'neurovirulent' named entity 'cleavage' named entity 'syncytium' named entity 'constructed' named entity 'protein' named entity 'Serotype' named entity 'FUSION PROTEIN' named entity 'IN VITRO' named entity 'PROTEIN CLEAVAGE' named entity 'SIGNIFICANT' named entity 'CDNA' named entity 'CLEAVAGE' named entity 'VIRUS' named entity 'VIRUSES' named entity 'CLONED' named entity 'DUCKS' named entity 'INCREASED' named entity 'THESE' named entity 'PROTEIN ' named entity 'PROTEASE' named entity 'IN VITRO' named entity 'SINGLE' named entity 'RECOVERY' named entity 'MUTANT' named entity 'PROTEIN CLEAVAGE' named entity 'IMPORTANT' named entity 'RESTRICTED' named entity 'SPECIES' named entity 'LIMITING' named entity 'MUTATIONS' named entity 'BASIC AMINO ACID' named entity 'FOUND' named entity 'OBSERVED' named entity 'VIRAL' named entity 'SITE OF' named entity 'PATHOGENICITY' named entity 'CHICKENS' named entity 'MUTATIONS' named entity 'HIGHLY' named entity 'SYNCYTIUM FORMATION' named entity 'BASIC AMINO ACIDS' named entity 'OLD' named entity 'RECOVERED' named entity 'TROPISM' named entity 'SEQUENCE' named entity 'MIMIC' named entity 'MOTIF' named entity 'STRAINS' named entity 'AVIRULENT' named entity 'TO CONTAIN' named entity 'IN VIVO' named entity 'ANALYSIS' named entity 'NUMBERS' named entity 'SITE' named entity 'PARAMYXOVIRUSES' named entity 'DAY-OLD' named entity 'AVIAN' named entity 'EVALUATE' named entity 'GROWTH CHARACTERISTICS' named entity 'POSITION' named entity 'RESULTS' named entity 'RECOMBINANT' named entity 'INFECTIOUS' named entity 'EGGS' named entity 'BIOLOGICAL' named entity 'REPLICATED' named entity 'CHICKEN' named entity 'CHICKENS' named entity 'GLUTAMINE RESIDUE' named entity 'CONTAINING' named entity 'PARENTAL' named entity 'CONSTRUCTED'

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