About: Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line

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About: Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line
Creator	Brouard, Jacques Corbet, Sandrine Vabret, Astrid Rozenberg, Flore Lebon, Pierre Dina, Julia Legrand, Loïc Ois Freymuth, Franç Lle Petitjean, Joë Phanie Gouarin, Sté
Source	Medline; PMC
abstract	Detection of viral antigens and isolation methods has long been used for the diagnosis of respiratory virus infections. The objective was to determine the ability of HuH7 cells to support the replication of prototype and wild strains of respiratory viruses. The cell culture‐adapted strains of influenza viruses A and B, parainfluenza viruses 1–4, respiratory syncytial viruses A and B, both strains of the human metapneumoviruses, numerous rhinoviruses, most of the adenoviruses, coronaviruses 229E and OC43, and a number of enteroviruses (poliovirus type 3, coxsackie virus B1, echovirus type 30) replicate in HuH7. The kinetic study of the replication of influenza A and B viruses showed that there were infected cells in HuH7 and MDCK lines as early as 24 hr post‐infection. However, the replication of influenza A and B viruses was more rapid and intense on MDCK cells than on HuH7 cells. During the three winters of 1999–2000, 2000–2001, and 2001–2002, of the 1,226 (23.3%) direct fluorescent assay‐positive nasal aspirates from children admitted to hospital, 788 were positive for respiratory syncytial virus, 228 for influenza virus, 133 for parainfluenza virus, and 77 for adenovirus. Of the 4,032 direct fluorescent assay‐negative nasal aspirates, 571 virus isolates were identified by using HuH7 cell culture; 272 rhinoviruses, 100 influenza viruses A and B, 85 enteroviruses, 40 adenoviruses, 35 coronaviruses, 31 parainfluenza viruses, and 10 respiratory syncytial viruses. Interestingly, 100/328 (30.5%) influenza viruses A and B, 40/189 (21.1%) adenoviruses, and 31/164 (19%) parainfluenza viruses type 1–3, not detected by direct fluorescent assay, were identified by isolation in HuH7 cell culture. J. Med. Virol. 77:295–301, 2005. © 2005 Wiley‐Liss, Inc.
has issue date	2005-08-24(xsd:dateTime)
bibo:doi	10.1002/jmv.20449
bibo:pmid	16121382
has license	no-cc
sha1sum (hex)	59c701ddaa9d99b60d49d32e9b05a1b74b411015
schema:url	https://doi.org/10.1002/jmv.20449
resource representing a document's title	Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line
has PubMed Central identifier	PMC7166344
has PubMed identifier	16121382
schema:publication	J Med Virol
resource representing a document's body	covid:59c701ddaa9d99b60d49d32e9b05a1b74b411015#body_text
is schema:about of	named entity 'Line' named entity 'Replication' named entity 'Virus' named entity 'Coronavirus' named entity 'Rhinovirus' named entity 'Cell Line' named entity 'adenoviruses' named entity 'viruses' named entity 'influenza A virus' named entity 'gentamycin' named entity 'cytopathologic' named entity 'cell lines' named entity 'France' named entity 'slow growth' named entity 'centrifuged' named entity 'trypsin' named entity 'HuH7' named entity 'coronavirus' named entity 'virus' named entity 'MDCK' named entity 'cell culture' named entity 'influenza A virus' named entity 'viruses' named entity 'pediatric wards' named entity 'respiratory syncytial viruses' named entity 'adenoviruses' named entity 'adenoviruses' named entity 'immunological' named entity 'Meylan' named entity 'adenoviruses' named entity 'real-time RT-PCR' named entity 'cell cultures' named entity 'influenza' named entity 'influenza B virus' named entity 'small cells' named entity 'influenza viruses' named entity 'PCR techniques' named entity 'fluorescent' named entity '229E' named entity 'supernatants' named entity 'NL63' named entity 'parainfluenza virus' named entity 'respiratory syncytial viruses' named entity 'influenza A virus' named entity 'human embryonic' named entity 'respiratory syncytial viruses' named entity 'respiratory syncytial viruses' named entity 'OC43' named entity 'viruses' named entity 'Panama' named entity 'cell line' named entity 'HuH7' named entity 'type 1' named entity 'influenza A virus' named entity 'Hierholzer' named entity 'viruses' named entity 'coronaviruses' named entity 'penicillin' named entity 'parainfluenza virus' named entity 'respiratory tract' named entity 'HepG2' named entity 'viruses' named entity 'enteroviruses' named entity 'respiratory syncytial viruses' named entity 'fluorescent' named entity 'HuH7' named entity 'NCI' named entity 'H1N1' named entity 'adenoviruses' named entity 'rhinovirus' named entity 'primers' named entity 'echovirus'

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