About: In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F(0)F(1)-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F(0)F(1)-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F(0)F(1)-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.   Goto Sponge  NotDistinct  Permalink

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  • In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F(0)F(1)-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F(0)F(1)-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F(0)F(1)-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.
Subject
  • Virology
  • RNA splicing
  • Virus genera
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