About: Protective Effects of Cell-Free Supernatant and Live Lactic Acid Bacteria Isolated from Thai Pigs Against a Pandemic Strain of Porcine Epidemic Diarrhea Virus

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About: Protective Effects of Cell-Free Supernatant and Live Lactic Acid Bacteria Isolated from Thai Pigs Against a Pandemic Strain of Porcine Epidemic Diarrhea Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Protective Effects of Cell-Free Supernatant and Live Lactic Acid Bacteria Isolated from Thai Pigs Against a Pandemic Strain of Porcine Epidemic Diarrhea Virus
Creator	Nilubol, Dachrit Temeeyasen, Gun Prapasarakul, Nuvee Sirichokchatchawan, Wandee
source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) is a coronavirus which causes severe diarrhea and fatal dehydration in piglets. In general, probiotic supplements could enhance recovery and protect piglets against enteric pathogens. Seven local lactic acid bacteria (LAB), (Ent. faecium 79N and 40N, Lact. plantarum 22F, 25F and 31F, Ped. acidilactici 72N and Ped. pentosaceus 77F) from pig feces were well-characterized as high potential probiotics. Cell-free supernatants (CFS) and live LAB were evaluated for antiviral activities by co-incubation on Vero cells and challenged with a pandemic strain of PEDV isolated from pigs in Thailand. Cell survival and viral inhibition were determined by cytopathic effect (CPE) reduction assay and confirmed by immunofluorescence. At 1:16, CFS dilution (pH 6.3–6.8) showed no cytotoxicity in Vero cells and was therefore used as the dilution for antiviral assays. The diluted CFS of all Lact. plantarum showed the antiviral effect against PEDV; however, the same antiviral effect could not be observed in Ent. faecium and Pediococcus strains. In competitive experiment, only live Lact. plantarum 25F and Ped. pentosaceus 77F showed CPE reduction in the viral infected cells to <50% observed field area. This study concluded that the CFS of all tested lactobacilli, and live Lact. plantarum (22F and 25F) and Pediococcus strains 72N and 77F could reduce infectivity of the pandemic strain of PEDV from pigs in Thailand on the target Vero cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12602-017-9281-y) contains supplementary material, which is available to authorized users.
has issue date	2017-04-22(xsd:dateTime)
bibo:doi	10.1007/s12602-017-9281-y
bibo:pmid	28434154
has license	no-cc
sha1sum (hex)	5a0abcbe02df183214680d6f23c3edce2cb7dee1
schema:url	https://doi.org/10.1007/s12602-017-9281-y
resource representing a document's title	Protective Effects of Cell-Free Supernatant and Live Lactic Acid Bacteria Isolated from Thai Pigs Against a Pandemic Strain of Porcine Epidemic Diarrhea Virus
has PubMed Central identifier	PMC7091344
has PubMed identifier	28434154
schema:publication	Probiotics Antimicrob Proteins
resource representing a document's body	covid:5a0abcbe02df183214680d6f23c3edce2cb7dee1#body_text
is schema:about of	named entity 'ISOLATED' named entity 'assay' named entity 'immunofluorescence' named entity 'assays' named entity 'Pandemic' named entity 'Thai' named entity 'LIVE' named entity 'EFFECTS' named entity 'COMPETITIVE' named entity 'TARGET' named entity 'LAB' named entity 'FATAL' named entity 'DEHYDRATION' named entity 'CELLS' named entity 'OBSERVED' named entity 'ISOLATED' named entity 'FECES' named entity 'PANDEMIC' named entity 'PIGS' named entity 'INFECTED' named entity 'DILUTED' named entity 'IMMUNOFLUORESCENCE' named entity 'LACTIC ACID BACTERIA' named entity 'EXPERIMENT' named entity 'CORONAVIRUS' named entity 'LACTIC ACID BACTERIA' named entity 'CPE' named entity 'TESTED' named entity 'ENHANCE' named entity 'FREE' named entity 'EFFECT' named entity 'STRAIN' named entity 'STRAIN' named entity 'PANDEMIC' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'BACTERIA ' named entity 'FREE' named entity 'THAI' named entity 'ACTIVITIES' named entity 'USED' named entity 'INHIBITION' named entity 'SUPPLEMENTS' named entity 'IS A' named entity 'CELL SURVIVAL' named entity 'CELL' named entity 'PIG' named entity 'PATHOGENS' named entity 'EVALUATED' named entity 'GENERAL' named entity 'VIRAL' named entity 'BACTERIA ' named entity 'CAUSES' named entity 'ANTIVIRAL' named entity 'PROBIOTIC' named entity 'ENT' named entity 'CONFIRMED BY' named entity 'PEDIOCOCCUS' named entity 'POTENTIAL' named entity 'PROBIOTICS' named entity 'CELL' named entity 'ENTERIC' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'ASSAY' named entity 'STRAINS' named entity 'SEVEN' named entity 'STUDY' named entity 'CYTOTOXICITY' named entity 'THAILAND' named entity 'LOCAL' named entity '6.3' named entity 'FIELD AREA' named entity 'PED' named entity 'VERO CELLS' named entity 'CFS' named entity 'INFECTIVITY' named entity 'CHARACTERIZED' named entity 'HIGH' named entity 'RECOVERY'

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