About: Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

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About: Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens
Creator	Bangoura, Berit Seeber, Frank Maksimov, Pavlo Schares, Gereon Conraths, Franz Aubert, Dominique Fabian, Benedikt Hedar, Fatima Koethe, Martin Villena, Isabelle
Source	PMC
abstract	BACKGROUND: Free-ranging chickens are often infected with Toxoplasma gondii and seroconvert upon infection. This indicates environmental contamination with T. gondii. METHODS: Here, we established a bead-based multiplex assay (BBMA) using the Luminex technology for the detection of T. gondii infections in chickens. Recombinant biotinylated T. gondii surface antigen 1 (TgSAG1(bio)) bound to streptavidin-conjugated magnetic Luminex beads served as antigen. Serum antibodies were detected by a fluorophore-coupled secondary antibody. Beads of differing color codes were conjugated with anti-chicken IgY or chicken serum albumin and served for each sample as an internal positive or negative control, respectively. The assay was validated with sera from experimentally and naturally infected chickens. The results were compared to those from reference methods, including other serological tests, PCRs and bioassay in mice. RESULTS: In experimentally infected chickens, the vast majority (98.5%, n = 65/66) of birds tested seropositive in the BBMA. This included all chickens positive by magnetic-capture PCR (100%, n = 45/45). Most, but not all inoculated and TgSAG1(bio)-BBMA-positive chickens were also positive in two previously established TgSAG1-ELISAs (TgSAG1-ELISA(SL), n = 61/65; or TgSAG1-ELISA(SH), n = 60/65), or positive in an immunofluorescence assay (IFAT, n = 64/65) and in a modified agglutination test (MAT, n = 61/65). All non-inoculated control animals (n = 28/28, 100%) tested negative. In naturally exposed chickens, the TgSAG1(bio)-BBMA showed a high sensitivity (98.5%; 95% confidence interval, CI: 90.7–99.9%) and specificity (100%; 95% CI: 85.0–100%) relative to a reference standard established using ELISA, IFAT and MAT. Almost all naturally exposed chickens that were positive in bioassay or by PCR tested positive in the TgSAG1(bio)-BBMA (93.5%; 95% CI: 77.1–98.9%), while all bioassay- or PCR-negative chickens remained negative (100%; 95% CI: 85.0–100%). CONCLUSIONS: The TgSAG1(bio)-BBMA represents a suitable method for the detection of T. gondii infections in chickens with high sensitivity and specificity, which is comparable or even superior to other tests. Since assays based on this methodology allow for the simultaneous analysis of a single biological sample with respect to multiple analytes, the described assay may represent a component in future multiplex assays for broad serological monitoring of poultry and other farm animals for various pathogens. [Image: see text]
has issue date	2020-07-31(xsd:dateTime)
bibo:doi	10.1186/s13071-020-04244-6
has license	cc-by
sha1sum (hex)	5a0b2518d13fc209db8ad675ec44392d9617c672
schema:url	https://doi.org/10.1186/s13071-020-04244-6
resource representing a document's title	Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens
has PubMed Central identifier	PMC7393333
schema:publication	Parasit Vectors
resource representing a document's body	covid:5a0b2518d13fc209db8ad675ec44392d9617c672#body_text
is schema:about of	named entity 'infection' covid:arg/5a0b2518d13fc209db8ad675ec44392d9617c672 named entity 'Toxoplasma' named entity 'detection' named entity 'infection' named entity 'infection' named entity 'serological' named entity 'animal species' named entity '1:25' named entity 'fluorescence' named entity 'titer' named entity 'streptavidin' named entity 'Recombinant' named entity 'sonication' named entity 'oocysts' named entity 'seronegative' named entity 'antigenic' named entity '95% CI' named entity 'tissue cysts' named entity 'infection' named entity 'human sera' named entity '95% CI' named entity 'negative control' named entity 'knockout mice' named entity 'Bio-Rad' named entity 'tachyzoites' named entity 'PBS' named entity 'infection' named entity 'fluorescence' named entity 'sonicated' named entity 'negative control' named entity 'affinity chromatography' named entity '95% CI' named entity 'diagnostic characteristics' named entity 'ELISAs' named entity 'incubation' named entity 'serological' named entity 'risk factor' named entity 'saline solution' named entity 'PBS' named entity 'infection status' named entity 'TBN' named entity 'lysate' named entity 'false-negative' named entity 'tachyzoites' named entity 'sashimi' named entity 'sheep' named entity 'drumstick' named entity 'T. gondii' named entity 'Luminex' named entity 'MBP' named entity 'chicken' named entity 'human infection' named entity 'conformational epitope' named entity 'human infection' named entity 'serological' named entity 'oocyst' named entity 'drumstick' named entity 'parasite' named entity 'life-threatening disease' named entity 'quantitative PCR' named entity 'C-terminal' named entity '95% CI' named entity 'human infection' named entity '95% CI' named entity 'T. gondii' named entity 'T. gondii' named entity 'knockout mice' named entity 'serological' named entity 'serum samples' named entity 'pepsin' named entity 'sensitivity and specificity' named entity 'T. gondii' named entity 'parasite'

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