About: Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus

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About: Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus
Creator	Fang, Liurong Wang, Dang Xiao, Shaobo Dong, Nan Fang, Puxian Hong, Yingying Liu, Xiaorong Bi, Jing Ma, Panpan
source	Medline; PMC
abstract	Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus. Bioinformatics predicts that PDCoV encodes two accessory proteins (NS6 and NS7), the species-specific proteins for coronavirus. In this study, four mAbs against the predicted NS7 were prepared by using the purified recombinant NS7 protein. Indirect immunofluorescence assay demonstrated that all mAbs recognized cells transfected with an NS7 expression construct or infected with PDCoV. Western blot showed that NS7-specific mAbs recognized an additional protein band of about 12 kDa from PDCoV-infected cell lysates but not from cells with the ectopic expression of NS7. Detailed analysis suggested that this additional protein band represented a novel accessory protein, termed NS7a, a 100 amino acid polypeptide identical to the 3′ end of NS7. Moreover, NS7a is encoded by a separate subgenomic mRNA with a non-canonical transcription regulatory sequence. In summary, our results identified a third accessory protein encoded by PDCoV, which will enhance our understanding of PDCoV.
has issue date	2017-02-01(xsd:dateTime)
bibo:doi	10.1099/jgv.0.000690
bibo:pmid	27995863
has license	bronze-oa
sha1sum (hex)	5ab60bf8e603d6adf2df7a4e0a3f6e35cfcb317f
schema:url	https://doi.org/10.1099/jgv.0.000690
resource representing a document's title	Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus
has PubMed Central identifier	PMC7079566
has PubMed identifier	27995863
schema:publication	Journal of General Virology
resource representing a document's body	covid:5ab60bf8e603d6adf2df7a4e0a3f6e35cfcb317f#body_text
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