About: Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection

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About: Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection
Creator	Wolfs, Tom Jansen, Nicolaas Nl, Rossen@umcutrecht, J Van De Pol, Alma Van Loon, Anton Wa, John
Source	Medline; PMC
abstract	INTRODUCTION: The aetiology of lower respiratory tract infections in young children admitted to the paediatric intensive care unit (PICU) is often difficult to establish. However, most infections are believed to be caused by respiratory viruses. A diagnostic study was performed to compare conventional viral tests with the recently developed real-time PCR technique. METHOD: Samples from children aged under 5 years presenting to a tertiary PICU suspected of having a lower respiratory tract infection were tested using conventional methods (viral culture and immunofluorescence) and real-time PCR during the winter season from December 2004 to May 2005. Conventional methods were used to check for respiratory syncytial virus, influenzavirus, parainfluenzavirus 1–3, rhinoviruses and adenoviruses. Real-time PCR was used to test for respiratory syncytial virus, influenzavirus, parainfluenzavirus 1–4, rhinoviruses, adenoviruses, human coronaviruses OC43, NL63 and 229E, human metapneumovirus, Mycoplasma pneumoniae and Chlamydia pneumoniae. RESULTS: A total of 23 patients were included, of whom 11 (48%) were positive for a respiratory virus by conventional methods. Real-time PCR confirmed all of these positive results. In addition, real-time PCR identified 22 more viruses in 11 patients, yielding a total of 22 (96%) patients with a positive sample. More than one virus was detected in eight (35%) children. CONCLUSION: Real-time PCR for respiratory viruses was found to be a sensitive and reliable method in PICU patients with lower respiratory tract infection, increasing the diagnostic yield twofold compared to conventional methods.
has issue date	2006-04-12(xsd:dateTime)
bibo:doi	10.1186/cc4895
bibo:pmid	16611370
has license	cc-by
sha1sum (hex)	5afe948e33645324e3fb0fe4e4d27b1bba317364
schema:url	https://doi.org/10.1186/cc4895
resource representing a document's title	Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection
has PubMed Central identifier	PMC1550925
has PubMed identifier	16611370
schema:publication	Crit Care
resource representing a document's body	covid:5afe948e33645324e3fb0fe4e4d27b1bba317364#body_text
is schema:about of	named entity 'YOUNG CHILDREN' named entity 'tests' named entity 'intensive care unit' named entity 'VALUE' named entity 'DIAGNOSTIC' named entity 'VIRUSES' named entity 'AETIOLOGY' named entity 'RECENTLY' named entity 'PERFORMED' named entity 'TESTS' named entity 'VIRAL' named entity 'ADMITTED TO' named entity 'RESPIRATORY VIRUSES' named entity 'INFECTIONS' named entity 'DIFFICULT' named entity 'REAL-TIME PCR ' named entity 'TO COMPARE' named entity 'PAEDIATRIC INTENSIVE CARE UNIT' named entity 'ACCESS' named entity 'TO DETECT' named entity 'OPEN' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'LOWER RESPIRATORY TRACT INFECTION' named entity 'LOWER RESPIRATORY TRACT INFECTIONS' named entity 'PAEDIATRIC INTENSIVE CARE UNIT' named entity 'ESTABLISH' named entity 'CONVENTIONAL' named entity 'YOUNG CHILDREN' named entity 'ADMITTED TO' named entity 'DIAGNOSTIC STUDY' named entity 'TECHNIQUE' covid:arg/5afe948e33645324e3fb0fe4e4d27b1bba317364 named entity 'respiratory' named entity 'performed' named entity 'polymerase' named entity 'real-time PCR' named entity 'aetiology' named entity 'lower respiratory tract infections' named entity 'young children' named entity 'Real-time PCR' named entity 'viruses' named entity 'virus' named entity 'LRTI' named entity 'hMPV' named entity '1, 2' named entity 'LRTI' named entity 'viral culture' named entity 'Sputa' named entity 'Real-time PCR' named entity 'RSV' named entity 'pathogens' named entity 'real-time PCR' named entity 'PCR' named entity 'RSV' named entity 'viral infections' named entity 'bronchiolitis' named entity 'virus infections' named entity 'Chlamydia pneumoniae' named entity 'young children' named entity 'Mycoplasma pneumoniae' named entity 'bacterial infection' named entity 'PCR' named entity 'clinical practice' named entity 'real-time PCR' named entity 'gold standard' named entity 'random hexamer' named entity 'sputum' named entity 'paediatric' named entity 'adenoviruses' named entity 'nmol/l' named entity 'LRTI' named entity 'viral testing' named entity 'viruses' named entity 'virus' named entity 'Medical Ethical'

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