About: Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay

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About: Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay
Creator	Hosaka, Norimitsu Ichimura, Hiroshi Ishizaki, Azumi Kageyama, Seiji Ndembi, Nicaise Numazaki, Kei
Source	Elsevier; Medline; PMC
abstract	Abstract A rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the pol-integrase gene was developed to detect human immunodeficiency virus type 1 (HIV-1) group M. This HIV-1 RT-LAMP assay is simple and rapid, and amplification can be completed within 35min under isothermal conditions at 60°C. The 100% detection limit of HIV-1 RT-LAMP was determined using a standard strain (WHO HIV-1 [97/656]) in octuplicate and found to be 120 copies/ml. The RT-LAMP assay was evaluated for use for clinical diagnosis using plasma samples collected from 57 HIV-1-infected and 40 uninfected individuals in Cameroon, where highly divergent HIV-1 strains are prevalent. Of the 57 samples from infected individuals, 56 harbored group-M HIV-1 strains, such as subtypes A, B, G, F2, and circulating recombinant forms (CRFs) _01, _02, _09, _11, _13; all were RT-LAMP positive. One sample harboring group-O HIV-1 and the 40 HIV-1-uninfected samples were RT-LAMP negative. These findings indicate that HIV-1 RT-LAMP can detect HIV-1 group-M RNA from plasma samples rapidly and with high sensitivity and specificity. These data also suggest that this RT-LAMP assay can be useful for confirming HIV diagnosis, particularly in resource-limited settings.
has issue date	2009-05-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.01.004
bibo:pmid	19186193
has license	els-covid
sha1sum (hex)	5bc4d92dcd27012532757bc3b0c55b8dff1319b9
schema:url	https://doi.org/10.1016/j.jviromet.2009.01.004
resource representing a document's title	Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay
has PubMed Central identifier	PMC7112927
has PubMed identifier	19186193
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:5bc4d92dcd27012532757bc3b0c55b8dff1319b9#body_text
is schema:about of	named entity 'negative' named entity 'HIV-1' named entity 'plasma' named entity 'HIV-1' named entity 'high' named entity 'amplification' named entity 'Rapid' named entity 'SUGGEST' named entity 'AMPLIFICATION' named entity 'GROUP M' named entity 'GROUP' named entity 'MIN' named entity 'COMPLETED' named entity 'INFECTED' named entity 'SAMPLES' named entity 'EVALUATED' named entity '80%' named entity 'SAMPLE' named entity 'COLLECTED' named entity '120' named entity 'DETERMINED' named entity 'GROUP M' named entity 'SETTINGS' named entity 'REVERSE TRANSCRIPTION' named entity 'SIMPLE' named entity 'TARGETING' named entity 'PREVALENT' named entity '100%' named entity 'USING' named entity 'SUBTYPES' named entity 'HIV-1' named entity 'RAPID' named entity 'DETECTION' named entity 'HUMAN IMMUNODEFICIENCY VIRUS TYPE 1' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'ISOTHERMAL AMPLIFICATION ASSAY' named entity 'REVERSE TRANSCRIPTION' named entity '1 GROUP' named entity 'FOUND' named entity 'RAPID' named entity 'COPIES' named entity 'TO DETECT' named entity 'THESE' named entity 'RECOMBINANT' named entity 'RNA' named entity 'INDIVIDUALS' named entity 'FORMS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'STANDARD' named entity 'RESOURCE' named entity 'RAPIDLY' named entity 'DETECT' named entity 'DATA' named entity 'LIMITED' named entity 'CAMEROON' named entity 'STRAINS' named entity 'USE' named entity 'HUMAN IMMUNODEFICIENCY VIRUS TYPE 1' named entity 'CLINICAL DIAGNOSIS' named entity 'POSITIVE' named entity 'USEFUL' named entity 'NEGATIVE' named entity 'HARBORED' named entity 'RT-LAMP ASSAY' named entity 'WHERE' named entity 'PLASMA' named entity 'HIV DIAGNOSIS' named entity 'HIGHLY' named entity 'ASSAY' named entity 'DETECTION LIMIT' named entity 'STRAIN' named entity 'FINDINGS' named entity 'CIRCULATING' named entity 'LAMP' named entity 'POL' named entity 'CONFIRMING'

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